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Myocardial effects of angiotensin II compared to norepinephrine in an animal model of septic shock
Critical Care volume 26, Article number: 281 (2022)
Angiotensin II is one of the vasopressors available for use in septic shock. However, its effects on the septic myocardium remain unclear. The aim of the study was to compare the effects of angiotensin II and norepinephrine on cardiac function and myocardial oxygen consumption, inflammation and injury in experimental septic shock.
This randomized, open-label, controlled study was performed in 20 anesthetized and mechanically ventilated pigs. Septic shock was induced by fecal peritonitis in 16 animals, and four pigs served as shams. Resuscitation with fluids, antimicrobial therapy and abdominal drainage was initiated one hour after the onset of septic shock. Septic pigs were randomly allocated to receive one of the two drugs to maintain mean arterial pressure between 65 and 75 mmHg for 8 h.
There were no differences in MAP, cardiac output, heart rate, fluid balance or tissue perfusion indices in the two treatment groups but myocardial oxygen consumption was greater in the norepinephrine-treated animals. Myocardial mRNA expression of interleukin-6, interleukin-6 receptor, interleukin-1 alpha, and interleukin-1 beta was higher in the norepinephrine than in the angiotensin II group.
In septic shock, angiotensin II administration is associated with a similar level of cardiovascular resuscitation and less myocardial oxygen consumption, and inflammation compared to norepinephrine.
Septic shock remains a major problem in the intensive care unit, with an estimated frequency of 10.4% . Vasopressor therapy is a cornerstone of the complex medical management of patients with septic shock. Norepinephrine (NE) is the first-line vasopressor choice in these patients , increasing vascular tone by stimulating α-adrenergic receptors and myocardial contractility by stimulating β-adrenergic receptors . Adrenergic agents have various non-hemodynamic effects, including increased glycolysis and altered immune responses . NE can dysregulate the immune response by attenuating the production of pro-inflammatory mediators, such as interleukin (IL)-6 or tumor necrosis factor (TNF)-α, and increasing anti-inflammatory IL-10 production . Adrenergic receptor stimulation can increase myocardial oxygen consumption, downregulate β-adrenergic receptors and reverse adrenergic G protein coupling, resulting in an inhibitory response to catecholamines and impaired myocardial contractility, especially in case of prolonged administration [6,7,8]. These potential deleterious effects have led to the search for non-catecholaminergic drugs to reduce exposure to catecholamines [2, 9,10,11].
In the ATHOS III trial, adding synthetic angiotensin II (Ang II) to NE during septic shock resuscitation was associated with significantly increased arterial blood pressure compared to placebo . Ang II administration was also associated with an increased probability of survival in a subgroup of patients with catecholamine-resistant vasodilatory shock and high renin levels at baseline .
Ang II has been implicated in the pathophysiology of chronic cardiac disease with pro-inflammatory effects mediated by the angiotensin II receptor 1 (AT1R) [14,15,16]. The safety profile of Ang II therapy in sepsis is not fully defined, but it may increase systemic and myocardial inflammation and cardiomyocyte apoptosis, which are implicated in the pathophysiology of septic cardiomyopathy [17,18,19].
Since the effects of Ang II on septic cardiomyopathy, especially pro-inflammatory cardiomyopathy, remain unclear, we used a clinically relevant large animal model of septic shock to investigate the effects of Ang II on cardiac function, myocardial oxygenation, myocardial inflammation, injury and apoptosis.
The study protocol followed the EU Directive (2010/63/EU) for animal experiments and was approved by the local animal ethics committee (Comité Ethique du Bien-Être Animal; protocol number 724N) from the Université Libre de Bruxelles (ULB) in Brussels (Belgium). Experiments were performed in the Experimental Laboratory of Intensive Care of the ULB (LA1230406) and the ARRIVE guidelines and MQTiPSS recommendations for translational research in sepsis were followed [20, 21].
Animals were randomized in an open-label, controlled study based on an established model of septic shock [22, 23]. Twenty pigs (Sus scrofa domesticus, RA-SE Genetics, Belgium) weighing 49 ± 5 kg were randomized to fecal peritonitis (n = 16) or sham procedure (n = 4, consisting of anesthesia and surgical preparation without sepsis induction). Animals were fasted for 18 h prior to the start of the experiment with free access to water. Thereafter, they were sedated in their enclosure with an intramuscular injection of midazolam (1 mg/kg) and ketamine hydrochloride (20 mg/kg) in the neck. After transportation to the operating room, a peripheral line was placed in a vein of the ear and a 4.5 F arterial catheter (Terumo Medical Company, Belgium) was placed in the left common femoral artery for invasive monitoring of arterial pressure and blood sampling. Following anesthesia induction with an intravenous injection of 3 μg/kg of sufentanyl, 1 mg/kg of propofol and 0.5 mg/kg of rocuronium, endotracheal intubation was performed; general anesthesia was achieved with continuous inhalation of sevoflurane (at 1.8 to 2.5% alveolar concentration) and analgesia with continuous infusion of morphine (0.2–0.5 mg/kg/h, the optimal dose being determined through repeated pain tests, i.e., change in heart rate or blood pressure after nasal septum pinching), in association with rocuronium. Volume-controlled mechanical ventilation (Primus, Draëger, Lübeck, Germany) was applied with a fixed tidal volume of 8 mL/kg, a positive end-expiratory pressure of 5 cmH2O, a fraction of inspired oxygen (FiO2) adjusted to keep PaO2 > 90 mmHg, and a respiratory rate adjusted to maintain an arterial pH between 7.35 and 7.45. For drug infusion, a three-lumen central venous catheter (Terumo Medical Company, Belgium) was inserted percutaneously into the right external jugular vein under ultrasound guidance (Vivid E90, GE Machines, USA).
A pulmonary artery catheter (CCO, Edwards LifeSciences, Irvine, California, USA) was advanced through the left external jugular vein into the pulmonary artery for measurement of right heart pressures and continuous monitoring of cardiac output (CO) and mixed venous oxygen saturation (SvO2). The electrocardiogram, intravascular pressures and CO were continuously displayed (SC9000, Siemens, Munich, Germany) and exported to an A/D recording station (Notocord-Hem 4.4, Notocord, France). A pressure sensor catheter (Millar® 5F Pressure Catheter, Texas, USA) was introduced in the right common femoral artery. Pulse pressure variation (PPV) was automatically calculated from the arterial femoral signal using the formula “PPV = PPmax – PPmin / (PPmax + Ppmin) / 2,” with PP being the pulse pressure (i.e., the difference between systolic and diastolic arterial pressures). A left ventricular (LV) pressure volume catheter (5 Fr, Transonic® Europe BV, Elsloo, The Netherlands) was inserted into the LV through the internal carotid artery and was connected to an ADV500 system (Transonic® Europe BV).
Fluid maintenance was achieved using a balanced crystalloid solution (Plasmalyte, Baxter, USA) at a perfusion rate of 5 to 10 mL/kg/h, aiming to maintain the PPV < 13% . Hypoglycemia was avoided by continuous infusion of a 20% glucose solution (1 to 2 mL/kg/h). A 14 Fr Foley catheter was surgically introduced into the bladder via a supra-pubic mini-laparotomy to monitor urine output and intravesical pressure (IVP). Finally, two abdominal drains were placed on each side of the abdominal cavity for the later introduction of autologous feces.
The experimental protocol and study time-points are shown in Fig. 1. Briefly, the baseline time-point was considered as the moment 2 h after the end of the instrumentation, when hemodynamic stabilization had been achieved. Sepsis was then induced by an intraperitoneal instillation of 3 g/kg of autologous feces, previously collected from the animal’s enclosure and diluted in 300 mL of 5% glucose solution. The maintenance infusion rate was reduced to 1 mL/kg/h until the animal developed severe hypotension, arbitrarily defined as a mean arterial pressure (MAP) between 45 and 50 mmHg (corresponding to the sepsis time-point). Severe hypotension was left untreated for one hour, and the end of this period was defined as the septic shock time-point. Fluid resuscitation was then started with 10 mL/kg/h of balanced crystalloid and 10 mL/kg/h of colloid (Geloplasma, Fresenius Kabi, France), with the objective to restore the PPV to < 13% or MAP ≥ 65 mmHg. Achievement of this objective was considered as the fluids time-point. At this point, the peritoneal drains were opened to remove peritoneal liquid and antimicrobial therapy was started, consisting of an intravenous administration of 2 g of amoxicillin-clavulanic acid every 8 h.
At this stage, animals were randomly allocated to administration of NE or Ang II (Sigma-Aldrich, St. Louis, USA); continuous infusion of both drugs was titrated to achieve a MAP of 65–75 mmHg. The last two, vasopressor, time-points (VP1 and VP2) were reached after 3 and 8 h of vasopressor administration, respectively.
The abdominal wall was opened surgically (without opening the peritoneum) in all the animals when the IVP increased to ≥ 12 mmHg, to limit an excessive increase in intra-abdominal pressure (IAP), which could have resulted in abdominal compartment syndrome .
After completion of the experiment, the animals were euthanized with 40 mL of 7.5% potassium chloride injection under deep anesthesia. Autopsies were then rapidly performed. Myocardial samples from the free LV wall were stored in an RNA later solution (Invitrogen™, RNAlater™ Stabilization Solution, ThermoFisher Scientific, MA, USA) for biological evaluation, flash frozen in liquid nitrogen, or embedded in paraffin after overnight fixation in formaldehyde for immunohistochemistry.
LV pressure–volume (PV) loop assessment (as illustrated in Additional file 1: Figure S1) and derived indices, blood sample handling, biomarker quantification, and molecular biology assays (including evaluation of myocardial apoptosis and mRNA and protein expression levels) are described in the supplemental digital content (Additional file 1: Table S1).
All analyses were predefined. All data are presented as mean ± sd (standard deviation) or median [25–75%] unless otherwise stated. To take into account the repeated measurements structure of the data, linear mixed-effects polynomial regression models with restricted maximum likelihood estimation (REML) and first-order autoregressive covariance structure (AR1) were used to examine the differences in all analyzed variables among the groups at the different considered time-points. The group and the time-point were considered as fixed effects in the fitted model. Interaction effects between groups and time were also tested. Post hoc multiple comparison procedure using Tukey HSD test was considered. This test allows for all possible pairwise comparisons while keeping the family-wise error low. Model checking was performed by inspection of residual and normal plots. When the normality of the residuals was rejected, the analyzed variable was log-transformed to fit the normality requirement of the mixed model. Multiple imputation was used to impute missing values. All statistical tests were two-tailed, and a p < 0.05 was considered statistically significant. Data were analyzed using Prism (GraphPad Software Inc., USA) and R software (R Foundation for Statistical Computing, Vienna, Austria).
Septic shock induction
All 16 animals in the intervention groups developed severe hypotension and tachycardia, associated with decreased SvO2 and increased veno-arterial CO2 partial pressure difference (PCO2 gap) compared to baseline (Tables 1 and 2). The mean time to reach the Sepsis time-point was similar in the two treatment groups (5.9 ± 1.4 h for NE vs. 5.7 ± 1.4 h for Ang II, p = 0.97). There were no statistically significant differences in hemodynamic, respiratory or biological variables between the treatment groups until the administration of the vasopressor (Fig. 2, Tables 1 and 2, Additional file 1: Tables S4, S5, and S6). The mean dose of NE required to maintain the MAP between 65 and 75 mmHg was 0.58 ± 0.40 microg/kg/min at VP1 and 0.80 ± 0.52 microg/kg/min at VP2. The mean Ang II dose was 261 ± 125 ng/kg/min at VP1 and 1051 ± 775 ng/kg/min at VP2. One animal developed atrial fibrillation in the Ang II group. The total cumulative fluid balance between baseline and the end of the experiment was 161 ± 30 mL/kg with NE and 150 ± 39 mL/kg with Ang II. The experiment lasted 17.7 ± 1.6 h in the NE group and 17.7 ± 1.3 h in the Ang II group (since BL to the euthanasia).
Global hemodynamics and tissue perfusion indices
After fluid resuscitation and during vasopressor therapy, MAP was maintained between 65 and 75 mmHg in all treated animals with a mean value of 69 ± 3 mmHg during the eight-hour exposure. Fluid administration and vasopressor therapy restored SvO2 and CO2 gap to within normal values in the two groups (Table 2). There were no statistically significant differences in CO, HR, or stroke volume (SV) over time in the two intervention groups (Fig. 2 and Table 1).
LV contractility, assessed by the dP/dTmax, increased in both groups with the vasopressor infusion, and this increase persisted in both groups using the dP/dtmax/EDV ratio as a preload-independent contractility index (Table 1). dP/dtmax was higher with NE than with Ang II throughout the 8 h of vasopressor administration (Fig. 2). The LV PRSW and Emax increased under NE exposure (Table 1). The theoretical volume extrapolated from the ESVPR at 100 mmHg of LV pressure (V100) deceased under norepinephrine treatment, corresponding to an increase in contractility (Table 1).
Estimation of the myocardial volume oxygen consumption (MVO2), assessed using the “Triple product,” a surrogate for MVO2 taking into account heart rate, ventricular pressure maximal range, and ventricular contractility (via LV dP/dtmax), was larger with NE than with Ang II (Fig. 2).
There were no significant differences between the two groups in LV maximal elastance, arterial elastance, preload recruitable stroke work (PRSW), or other PV loop-derived indices (Table 1).
Time comparisons and comparison with the Sham group are shown in Additional file 1: Table S2–S3.
Evaluation of inflammation, myocardial mRNA expression, myocardial injury, and apoptosis
At the Fluids time-point, circulating levels of pro-inflammatory cytokines (TNF-α and IL-6) had increased similarly in the two treatment groups in response to sepsis (Table 2); Anti-inflammatory IL-10 levels were increased in the Ang II group at VP1 compared to baseline. Circulating IL-6 levels remained high in the NE group at VP1 and VP2, but decreased at VP2 in the Ang II group. Circulating levels of TNF-α were not statistically significantly different from baseline values at VP1 and VP2 (Table 2). IL-6/IL-10 and TNF-α/IL-10 ratios at the different time-points are shown in Fig. 3.
LV pro-inflammatory cytokines (expressed as mRNA expression of IL-6 and its receptor (IL-6R), IL1-α, and IL1-β) were upregulated in the NE compared to the Ang II group (Fig. 3). There were no statistically significant differences in mRNA expressions of TNF-α and IL-10 (Fig. 3). As shown in Additional file 1: Figure S2, mRNA expressions of intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) were higher in the NE than in the Ang II group. There were no statistically significant differences in ICAM-2 expression between the groups.
The activation of signal transducer and activator of transcription 3 (STAT3), the main transcription factor involved in IL-6-mediated signaling, herein evaluated by Tyr705 phosphorylation, were upregulated in NE—compared to Ang II-treated animals (Fig. 3).
Myocardial injury, assessed by high sensitive cardiac troponin I (hs-cTrop) release, was significantly higher at VP2 in animals receiving NE compared to sham animals (p = 0.0023), with a similar trend compared to Ang II (p = 0.06), (Additional file 1: Figure S3).
The LV pro-apoptotic Bax-to-Bcl2 mRNA expression ratio was higher in the Ang II than in the NE and sham groups (Additional file 1: Figure S2), but the LV apoptotic rate, assessed by TUNEL staining and evaluating the termination of apoptotic processes, was similar in the three groups (Additional file 1: Figure S2).
Myocardial mRNA and protein expression of adrenergic and angiotensin II receptors
LV mRNA and protein expressions of adrenergic receptor alpha 1 (α1-AR) were decreased in the two intervention groups compared to in sham animals, whereas adrenergic receptor beta 1 (β1-AR) mRNA and protein expressions were similar (Fig. 4A, B) to those in the sham group. There was no significant difference in Ang II receptor 1 (AT1R) or Ang II receptor 2 (AT2R) mRNA and protein expression between groups. AT2R mRNA and protein expressions were not affected by sepsis or vasopressor choice (Fig. 4), but there was a tendency for AT1R mRNA and protein expression to decrease in both intervention groups, mainly in the Ang II group (p value = 0.12 and 0.11 for Ang II vs. sham, for mRNA and protein expression, respectively). In addition, protein levels of Type-1 angiotensin II receptor-associated protein (AGTRAP), a negative regulator of the AT1R signaling pathway, also tended to be upregulated in the Ang II group (p = 0.07 and p = 0.21 compared to NE and sham groups, respectively).
In this experimental model of septic shock, Ang II administration combined with optimal fluid administration and antibiotic therapy resulted in a similar degree of cardiovascular resuscitation compared to NE administration in the first hours of septic shock. Load-independent contractility indices derived from PV loop assessment confirmed an intrinsic positive inotropic effect of Ang II in sepsis, with a lower myocardial oxygen consumption than NE. Cardiac inflammation, assessed by myocardial mRNA expression of inflammatory cytokines (IL-6 and its receptor, IL1-α and IL1-β), was upregulated in the NE compared to the Ang II group and STAT3 signaling was consistent with an IL-6 mediated pathway. AT2R mRNA and protein expressions were not affected by sepsis or vasopressor choice.
Administration of Ang II with fluids achieved the same resuscitation objectives and normalization of the tissue perfusion indices SvO2 and PCO2 gap as did administration of NE. Ang II was associated with a small increase in cardiac contractility, assessed by dP/dTmax, and this increase in contractility was persistent when using dP/dTmax/EDV ratio, a non-preload dependent index . The positive inotropic effect of Ang II has been shown in in vitro studies [29, 30] and one preclinical study in healthy pigs . As expected, cardiac contractility rapidly increased with NE, to a larger extent than in the Ang II group and persisted throughout the study. Nevertheless, MAP, CO and heart rate were similar in the two groups. This observation is consistent with a previous experimental study by Corrêa et al. conducted in a swine model of septic shock, in which there were no significant differences in MAP, CO, or SV between NE and Ang II groups, despite the same fluid resuscitation protocol . These studies highlight the importance of preload optimization with fluids in septic shock, especially when using drugs with a predominantly vasopressor effect ; indeed, Wan et al. showed that Ang II infusion alone decreased CO in a non-fluid resuscitated septic shock model . Another potentially beneficial effect of Ang II related to reducing catecholamine exposure is the finding that NE was associated with increased MVO2, as assessed using the “Triple product.” This observation suggests higher oxygen consumption with NE administration, with no beneficial effect on tissue perfusion, and may have important clinical implications, especially in patients with ischemic cardiomyopathy or other causes of impaired myocardial perfusion.
Catecholamines are known to have direct pro-inflammatory effects on the myocardium , but the observed differences in myocardial inflammation can also be explained by modulation of the renin-angiotensin pathway: Bellomo et al. showed that Ang II infusion reduced renin secretion in septic shock  and hypothesized that the decrease in renin secretion could modulate the immune response. The incubation of leukocytes with renin induces the production of pro-inflammatory cytokines, including IL-6 , and administration of a renin receptor blocker reduced the pro-inflammatory response and increased survival in a rodent model of sepsis induced by cecal ligation and puncture (CLP) .
Another hypothesis that could explain the difference in myocardial inflammation between groups is modulation via angiotensin receptors. Myocardial AT1R is downregulated during sepsis  and we showed that AT2R myocardial mRNA and protein expression were not affected by the vasopressor choice. Moreover, myocardial levels of AGTRAP tended to be upregulated in the Ang II group. We hypothesize that when Ang II is used, the imbalance between myocardial AT1R and AT2R during sepsis may lead to a predominant AT2R-mediated pathway, responsible for an anti-inflammatory effect . This result is in accordance with previous data showing sepsis-induced reduction in the AT1R/AT2R ratio in human arterial samples exposed to septic stimuli . Finally, it has been shown recently that angiotensin-converting enzyme 2 (ACE2), which transforms Ang II into angiotensin (1–7) (Ang-(1–7)), an anti-inflammatory peptide, was increased during acute respiratory distress syndrome  and an increase in Ang-(1–7) concentration was also observed. Hence, we can postulate that a similar decrease in the ACE/ACE2 ratio may occur during sepsis [41, 42], resulting in degradation of Ang II and an anti-inflammatory response mediated by Ang-(1–7), which could be restored by synthetic Ang II perfusion. Moreover, Ang II can also be transformed into alamandine via a double step process through decarboxylases and ACE2, which can also modulate anti-inflammatory properties .
These findings, associated with a trend to less myocardial injury as assessed by hs-cTrop release, may be related to an improvement in cardiovascular outcomes after sepsis .
The increase in mRNA expression of the Bax/Bcl2 ratio is consistent with the imbalance between AT1R/AT2R, as AT2R stimulation is known to have pro-apoptotic effects . Nevertheless, terminal apoptosis assessed using TUNEL staining was similar between groups, which suggests that the short exposure period was not sufficient to induce apoptosis in this model.
Dynamic parameters are recommended to assess fluid responsiveness, and here, fluid administration was titrated to maintain a PPV < 13% when hypotension occurred . This was associated with a high fluid balance observed in both groups and explained by the severity of the model. When compared to others studies, fluid administration in swine models is generally high and comparable with our study [48, 49]. The objective of the fluid protocol used here was to optimize preload similarly in both groups, with a PPV < 13% when hypotension occurred, which is not necessarily associated with fluid unresponsiveness .The IVP was also monitored and abdominal wall opened to avoid any interaction of increased IAP on hemodynamic management . Instrumentation was performed with a closed chest and pericardium and according to a minimally invasive approach; moreover, as all the catheters were introduced percutaneously under ultrasound guidance, tissue inflammation related to surgery was limited. This model follows other recommendations of the Surviving Sepsis Campaign, including abdominal drainage, adapted antimicrobial therapy, and early vasopressor introduction . We used polymicrobial sepsis as can be observed in humans, which induced severe multiple organ dysfunction. Nevertheless, our study has several limitations: First, due to species differences with humans, normal lactate levels are usually observed in swine septic shock [48,49,50] despite developing all the criteria for a septic shock and tissular hypoperfusion . Second, the limited number of animals may reduce the probability of showing differences between groups and the open label design could expose a selection bias, but randomization was performed before the start of the experiment and baseline characteristics were well balanced in all groups. Third, the relatively early development of sepsis might explain the absence of significant septic cardiomyopathy and overt molecular changes, limiting our ability to make inferences about the impact of this therapeutic strategy in this situation. However, one could argue that treatment in the early phase of septic shock is of paramount importance in determining the later development of septic cardiomyopathy. In addition, our study was focused on norepinephrine, which has alpha- and beta-adrenergic effects. Here, the study cannot separate these different mechanisms, and a third group with a pure alpha vasopressor, as phenylephrine, would have better clarified the contribution of the different pathways but against SSC recommendations. Finally, the high ang II dose used in our study is similar to that observed in a similar septic shock swine study  but higher compared to the average dose used in the ATHOS III study . However, in the ATHOS III study, Ang II was used in association with norepinephrine and the vasoplegia in these patients was lower with a median norepinephrine requirement of 0.34 microg/kg/min at baseline, compared to 0.80 microg/kg/min at VP2 in our experimental model . We investigated the use of Ang II as a single vasopressor agent, instead of in association with NE, but this enabled us to better analyze the specific effects of Ang II.
These results have several implications for vasopressor use in septic shock, especially regarding the safety concerns of Ang II on myocardial inflammation: By reducing renin secretion and stimulating AT2R, Ang II could have beneficial effects on local inflammation [13, 38], in addition to the beneficial effects of reducing catecholamine exposure, which can otherwise contribute to impaired cardiac contractility by β-adrenergic downregulation [8, 52] and higher MVO2.
In conclusion, in a resuscitated large animal model of septic shock, Ang II administration can restore organ perfusion as efficiently as can NE, resulting in a similar CO, heart rate and MAP, but with less MVO2, and inflammation compared to treatment with NE.
Availability of the data materials
The datasets used during the current study are available from the corresponding author on reasonable request.
Vincent J-L, Jones G, David S, Olariu E, Cadwell KK. Frequency and mortality of septic shock in Europe and North America: a systematic review and meta-analysis. Crit Care. 2019;23:196.
Evans L, Rhodes A, Alhazzani W, Antonelli M, Coopersmith CM, French C, et al. Surviving sepsis campaign: international guidelines for management of sepsis and septic shock 2021. Crit Care Med. 2021;Publish Ahead of Print.
Beurton A, Ducrocq N, Auchet T, Joineau-Groubatch F, Falanga A, Kimmoun A, et al. Beneficial effects of norepinephrine alone on cardiovascular function and tissue oxygenation in a pig model of cardiogenic shock. Shock. 2016;46:214–8.
Barth E, Albuszies G, Baumgart K, Matejovic M, Wachter U, Vogt J, et al. Glucose metabolism and catecholamines. Crit Care Med. 2007;35:S508–18.
Stolk R, van der Pasch E, Naumann F, Schouwstra J, Bressers S, van Herwaarden T, et al. Norepinephrine dysregulates the immune response and compromises host defense during sepsis. Am J Resp Crit Care. 2020;202:830–42.
Hollenberg SM, Singer M. Pathophysiology of sepsis-induced cardiomyopathy. Nat Rev Cardiol. 2021;18:1–11.
Lyon AR, Rees PS, Prasad S, Poole-Wilson PA, Harding SE. Stress (Takotsubo) cardiomyopathy—a novel pathophysiological hypothesis to explain catecholamine-induced acute myocardial stunning. Nat Clin Pract Card. 2008;5:22–9.
Carrara M, Ferrario M, Pinto BB, Herpain A. The autonomic nervous system in septic shock and its role as a future therapeutic target: a narrative review. Ann Intensive Care. 2021;11:80.
Asfar P, Chawla L, Lerolle N, Radermacher P. Angiotensin-II: more than just another vasoconstrictor to treat septic shock-induced hypotension?*. Crit Care Med. 2014;42:1961–3.
Kimmoun A, Ducrocq N, Levy B. Mechanisms of vascular hyporesponsiveness in septic shock. Curr Vasc Pharmacol. 2013;11:139–49.
Levy B, Fritz C, Tahon E, Jacquot A, Auchet T, Kimmoun A. Vasoplegia treatments: the past, the present, and the future. Crit Care Lond Engl. 2018;22:52.
Khanna A, English SW, Wang XS, Ham K, Tumlin J, Szerlip H, et al. Angiotensin II for the treatment of vasodilatory shock. New Engl J Med. 2017;377:419–30.
Bellomo R, Forni LG, Busse LW, McCurdy MT, Ham KR, Boldt DW, et al. Renin and survival in patients given angiotensin II for catecholamine-resistant vasodilatory shock. A clinical trial. Am J Resp Crit Care. 2020;202:1253–61.
Weber KT. Extracellular matrix remodeling in heart failure: a role for De Novo angiotensin II generation. Circulation. 1997;96:4065–82.
Jacques D, Provost C, Normand A, Abdallah NA, Al-Khoury J, Bkaily G. Angiotensin II induces apoptosis of human right and left ventricular endocardial endothelial cells by activating the AT2 receptor1. Can J Physiol Pharm. 2019;97:581–8.
Mehta PK, Griendling KK. Angiotensin II cell signaling: physiological and pathological effects in the cardiovascular system. Am J Physiol Cell Physiol. 2007;292:C82-97.
Buchtele N, Schwameis M, Jilma B. Angiotensin II for the treatment of vasodilatory shock: enough data to consider angiotensin II safe? Crit Care. 2018;22:96.
Antonucci E, Gleeson PJ, Annoni F, Agosta S, Orlando S, Taccone FS, et al. Angiotensin II in refractory septic shock. Shock. 2017;47:560–6.
Ikeda U, Maeda Y, Kawahara Y, Yokoyama M, Shimada K. Angiotensin II augments cytokine-stimulated nitric oxide synthesis in rat cardiac myocytes. Circulation. 1995;92:2683–9.
Osuchowski MF, Ayala A, Bahrami S, Bauer M, Boros M, Cavaillon J-M, et al. Minimum quality threshold in pre-clinical sepsis studies (MQTiPSS). Shock. 2018;50:377–80.
du Sert NP, Hurst V, Ahluwalia A, Alam S, Avey MT, Baker M, et al. The ARRIVE guidelines 2.0: updated guidelines for reporting animal research. Plos Biol. 2020;18:e3000410.
Carrara M, Herpain A, Baselli G, Ferrario M. A mathematical model of dPdt max for the evaluation of the dynamic control of heart contractility in septic shock. IEEE Trans Bio-Med Eng. 2018;66:2719–27.
Carrara M, Herpain A, Baselli G, Ferrario M. Vascular decoupling in septic shock: the combined role of autonomic nervous system, arterial stiffness, and peripheral vascular tone. Front Physiol. 2020;11:594.
Teboul J-L, Monnet X, Chemla D, Michard F. Arterial pulse pressure variation with mechanical ventilation. Am J Resp Crit Care. 2019;199:22–31.
Kirkpatrick AW, Roberts DJ, Waele JD, Jaeschke R, Malbrain MLNG, et al. Intra-abdominal hypertension and the abdominal compartment syndrome: updated consensus definitions and clinical practice guidelines from the World Society of the Abdominal Compartment Syndrome. Intens Care Med. 2013;39:1190–206.
Bernstein RD, Ochoa FY, Xu X, Forfia P, Shen W, Thompson CI, et al. Function and production of nitric oxide in the coronary circulation of the conscious dog during exercise. Circ Res. 1996;79:840–8.
Setty S, Tune JD, Downey HF. Nitric oxide modulates right ventricular flow and oxygen consumption during norepinephrine infusion. Am J Physiol Heart C. 2002;282:H696-703.
Kass DA, Maughan WL, Guo ZM, Kono A, Sunagawa K, Sagawa K. Comparative influence of load versus inotropic states on indexes of ventricular contractility: experimental and theoretical analysis based on pressure-volume relationships. Circulation. 2018;76:1422–36.
Moravec CS, Schluchter MD, Paranandi L, Czerska B, Stewart RW, Rosenkranz E, et al. Inotropic effects of angiotensin II on human cardiac muscle in vitro. Circulation. 2018;82:1973–84.
Rajagopal K, Whalen EJ, Violin JD, Stiber JA, Rosenberg PB, Premont RT, et al. β-Arrestin2-mediated inotropic effects of the angiotensin II type 1A receptor in isolated cardiac myocytes. Proc Natl Acad Sci. 2006;103:16284–9.
Broomé M, Haney M, Häggmark S, Johansson G, Åneman A, Biber B. Pressure-independent cardiac effects of angiotensin II in pigs. Acta Physiol Scand. 2004;182:111–9.
Corrêa TD, Jeger V, Pereira AJ, Takala J, Djafarzadeh S, Jakob SM. Angiotensin II in septic shock. Crit Care Med. 2014;42:e550–9.
Wan L, Langenberg C, Bellomo R, May CN. Angiotensin II in experimental hyperdynamic sepsis. Crit Care Lond Engl. 2009;13:R190.
Orso D, Copetti R. Adrenergic overstimulation A neglected mechanism of sepsis-related cardiomyopathy. Chest. 2019;155:647.
Narumi K, Hirose T, Sato E, Mori T, Kisu K, Ishikawa M, et al. A functional (pro)renin receptor is expressed in human lymphocytes and monocytes. Am J Physiol Renal. 2015;308:F487–99.
Hirano Y, Takeuchi H, Suda K, Hagiwara T, Miyasho T, Kawamura Y, et al. (Pro)renin receptor blocker improves survival of rats with sepsis. J Surg Res. 2014;186:269–77.
Bucher M, Ittner K-P, Hobbhahn J, Taeger K, Kurtz A. Downregulation of angiotensin II type 1 receptors during sepsis. Hypertension. 2001;38:177–82.
Kaschina E, Grzesiak A, Li J, Foryst-Ludwig A, Timm M, Rompe F, et al. Angiotensin II type 2 receptor stimulation. Circulation. 2008;118:2523–32.
Vasques-Nóvoa F, Laundos TL, Cerqueira RJ, Quina-Rodrigues C, Soares-dos-Reis R, Baganha F, et al. MicroRNA-155 amplifies nitric oxide & sol; cGMP signaling and impairs vascular angiotensin II reactivity in septic shock. Crit Care Med. 2018;46:e945–54.
Gerard L, Lecocq M, Bouzin C, Hoton D, Schmit G, Pereira JP, et al. Increased angiotensin-converting enzyme 2 and loss of alveolar type II cells in COVID-19-related acute respiratory distress syndrome. Am J Resp Crit Care. 2021;204:1024–34.
Picod A, Deniau B, Ayar PV, Genest M, Julian N, Azibani F, et al. Alteration of the renin-angiotensin-aldosterone system in shock: role of the dipeptidyl peptidase 3. Am J Resp Crit Care. 2020.
Chawla LS, Bellomo R. Reply to: Alteration of the renin-angiotensin-aldosterone system in shock: role of the dipeptidyl peptidase 3. Am J Resp Crit Care. 2020.
Schleifenbaum J. Alamandine and its receptor MrgD pair up to join the protective arm of the renin-angiotensin system. Front Med. 2019;6:107.
Garcia MA, Rucci JM, Thai KK, Lu Y, Kipnis P, Go AS, et al. Association between troponin I levels during sepsis and postsepsis cardiovascular complications. Am J Resp Crit Care. 2021;204:557–65.
Chow BSM, Allen TJ. Angiotensin II type 2 receptor (AT2R) in renal and cardiovascular disease. Clin Sci. 2016;130:1307–26.
Guillon A, Preau S, Aboab J, Azabou E, Jung B, et al. Preclinical septic shock research: why we need an animal ICU. Ann Intensive Care. 2019;9:66.
Hellman J, Bahrami S, Boros M, Chaudry IH, Fritsch G, Gozdzik W, et al. Part III: minimum quality threshold in preclinical sepsis studies (MQTiPSS) for fluid resuscitation and antimicrobial therapy endpoints. Shock Augusta Ga. 2019;51:33–43.
Simon F, Giudici R, Scheuerle A, Gröger M, Asfar P, Vogt JA, et al. Comparison of cardiac, hepatic, and renal effects of arginine vasopressin and noradrenaline during porcine fecal peritonitis: a randomized controlled trial. Crit Care. 2009;13:R113.
Krejci V, Hiltebrand LB, Jakob SM, Takala J, Sigurdsson GH. Vasopressin in septic shock: effects on pancreatic, renal, and hepatic blood flow. Crit Care. 2007;11:R129–R129.
Corrêa TD, Vuda M, Blaser AR, Takala J, Djafarzadeh S, Dünser MW, et al. Effect of treatment delay on disease severity and need for resuscitation in porcine fecal peritonitis. Crit Care Med. 2012;40:2841–9.
Singer M, Deutschman CS, Seymour CW, Shankar-Hari M, Annane D, Bauer M, et al. The third international consensus definitions for sepsis and septic shock (Sepsis-3). JAMA. 2016;315:801–10.
Bernardin G, Strosberg AD, Bernard A, Mattei M, Marullo S. β-adrenergic receptor-dependent and -independent stimulation of adenylate cyclase is impaired during severe sepsis in humans. Intens Care Med. 1998;24:1315–22.
The authors are grateful for the assistance of Emeline HUPKENS, Karen PICKETT, Angeline NOVA, Prof Vincent SOBANSKI, Sylvain MORINEAU, and Gregory VEGH.
This study was funded by national funds through FCT—Portuguese Foundation for Science and Technology, under the scope of the Cardiovascular R&D Center – UnIC (UIDB/00051/2020 and UIDP/00051/2020). B.G. was supported by a grant from “Année recherche, relations internationales, UFR3S, Université de Lille, France.”
Ethical approval and consent for publication
The study was approved by the local animal ethics committee (Comité Ethique du Bien-Être Animal; protocol number 724N) from the Université Libre de Bruxelles in Brussels (Belgium). Experiments were performed in the Experimental Laboratory of Intensive Care of the ULB (LA1230406).
The authors declare that they have no competing interests.
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. Figure S1. Pressure-volume loop illustration. Table S1. Primers used for real-time quantitative polymerase chain reaction (RTQ-PCR) in porcine myocardial tissue. Table S2. Hemodynamic variables in the three groups at the different study time-points. *p-value <0.05 between NE and Ang II. †p-value < 0.05 between NE and Sham. ‡p-value < 0.05 between Ang II and Sham. P-value < 0.05 compared to baseline for NE (§), Ang II (ll) and Sham (**) groups. HR: heart rate; MAP: mean arterial pressure; SV: stroke volume; CO: cardiac output; RAP: right atrial pressure; LVEDV: left ventricular end diastolic volume; LVESV: left ventricular end systolic volume; LVEDP: left ventricularend diastolic pressure; EF: ejection fraction; PRSW: preload recruitable stroke work; Emax: left ventricular maximal elastance; Ea: effective arterial elastance; Ea/Emax: left ventriculo-arterial coupling; V30: LV volume at 30 mmHg on the End Diastolic Pressure Volume Relationship; V100: LV volume at 100 mmHg on the End Systolic Pressure Volume Relationship; NE: norepinephrine; Ang: angiotensin PV loop analysis was obtained at baseline, fluids, vasopressor 1 and vasopressor 2. Table S3. Biological and oxygenation values in the three groups at the different study timepoints. *p-value <0.05 between NE and Ang II. †p-value < 0.05 between NE and Sham. ‡p-value < 0.05 between Ang II and Sham. p-value < 0.05 compared to baseline for NE (§), Ang II (ll) and Sham (**) groups. CO2 gap: veno-arterial difference in CO2 partial pressure; SVO2: mixed venous oxygen saturation; BE: base excess; IL: interleukin; TNF: tumor necrosis factor; NE: norepinephrine; Ang: angiotensin. Table S4. Respiratory variables. Results are presented as mean + SD. *p-value between NE and Ang II groups. †p-value < 0.05 between NE and Sham groups. ‡p-value < 0.05 between Ang II and Sham groups. p-value < 0.05 compared to baseline for NE (§), Ang II (ll) and Sham (**) groups. PaO2: Arterial partial pressure of oxygen; FiO2 fraction of oxygen inspired; Pplat: plateau pressure; Crs:compliance of the respiratory system; EtCO2: end-tidal carbon dioxide; PaCO2: arterial partial pressure of carbon dioxide. Table S5. Blood gas analysis. Values are presented as mean + SD. *p-value between NE and angiotensin II group. †p-value < 0.05 between NE and Sham group. ‡p-value < 0.05 between angiotensin II and Sham group. P-value < 0.05 compared to baseline for NE (§), angiotensin II (ll) and Sham (**) group. Hb: hemoglobin; Ht: hematocrit. PaO2: arterial partial pressure of oxygen. PaCO2: arterial partial pressure of carbon dioxide. Table S6. Biological variables. Values are expressed as mean ± SD. *p-value between NE and Ang II groups. †p-value < 0.05 between NE and Sham groups. ‡p-value < 0.05 between Ang II and Sham groups. p-value < 0.05 compared to baseline for NE (§), Ang II (ll) and Sham (**) groups. ASAT: Aspartate aminotransferase; ALAT Alanine aminotransferase; LDH: Lactate dehydrogenase. Figure S2. A. Left ventricular mRNA expression of molecules implicated in Ca2+ handling and contractile apparatus [ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (SERCA2A) and phospholamban (PLB)] B. LV mRNA expression of the ratio Bax/Bcl2 in norepinephrine (black bars) and angiotensin II groups. *pvalue< 0.05. C. Cardiac apoptotic rate: ratio of apoptotic nuclei (TUNEL-positive or brown nuclei) to total nuclei (brown+blue nuclei) (x100 to be expressed as a percentage). D. mRNA expression of cell adhesion molecules (ICAM1, 2 and VCAM1) and eNOS, iNOS, nNOS in norepinephrine and angiotensin II groups compared to sham group. Figure S3. Fold Changes expressed in % between baseline and vasopressor 2 time points
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Garcia, B., Su, F., Dewachter, L. et al. Myocardial effects of angiotensin II compared to norepinephrine in an animal model of septic shock. Crit Care 26, 281 (2022). https://doi.org/10.1186/s13054-022-04161-3
- Septic shock
- Septic cardiomyopathy
- Renin-angiotensin system