Fig. 4
From: Myocardial effects of angiotensin II compared to norepinephrine in an animal model of septic shock
Left ventricular myocardial adrenergic and angiotensin II receptor expression in the three groups. A: Left ventricular (LV) relative mRNA expression of angiotensin II receptor type 1 and 2 (AT1R and AT2R). LV relative mRNA expression of adrenergic receptor alpha 1 (α1-AR) and adrenergic receptor beta 1 (β1-AR). Relative quantification was achieved using the comparative 2−ΔΔCt method by normalization with the housekeeping gene (ActB). Results are expressed as relative fold increase above the mean value of LV relative mRNA expression of the sham group arbitrarily fixed at 1. *p < 0.05. NE: black boxes (n = 7); Ang II: blue boxes (n = 8); sham: white boxes (n = 3). B: Immunoblotting of LV samples of α1-AR, β1-AR, AT1R, and AT2R protein expressions. Values are expressed as mean ± standard deviation. C. LV adrenoreceptors and angiotensin receptors gels. Uncropped gels are available in the Additional file 2. *p < 0.05. NE: black boxes (n = 8); ATII: blue boxes (n = 8); sham: white boxes (n = 4)