- Open Access
Intensive care unit-acquired infection as a side effect of sedation
Critical Care volume 14, Article number: R30 (2010)
Sedative and analgesic medications are routinely used in mechanically ventilated patients. The aim of this review is to discus epidemiologic data that suggest a relationship between infection and sedation, to review available data for the potential causes and pathophysiology of this relationship, and to identify potential preventive measures.
Data for this review were identified through searches of PubMed, and from bibliographies of relevant articles.
Several epidemiologic studies suggested a link between sedation and ICU-acquired infection. Prolongation of exposure to risk factors for infection, microaspiration, gastrointestinal motility disturbances, microcirculatory effects are main mechanisms by which sedation may favour infection in critically ill patients. Furthermore, experimental evidence coming from studies both in humans and animals suggest that sedatives and analgesics present immunomodulatory properties that might alter the immunologic response to exogenous stimuli. Clinical studies comparing different sedative agents do not provide evidence to recommend the use of a particular agent to reduce ICU-acquired infection rate. However, sedation strategies aiming to reduce the duration of mechanical ventilation, such as daily interruption of sedatives or nursing-implementing sedation protocol, should be promoted. In addition, the use of short acting opioids, propofol, and dexmedetomidine is associated with shorter duration of mechanical ventilation and ICU stay, and might be helpful in reducing ICU-acquired infection rates.
Prolongation of exposure to risk factors for infection, microaspiration, gastrointestinal motility disturbances, microcirculatory effects, and immunomodulatory effects are main mechanisms by which sedation may favour infection in critically ill patients. Future studies should compare the effect of different sedative agents, and the impact of progressive opioid discontinuation compared with abrupt discontinuation on ICU-acquired infection rates.
Healthcare-associated infections are the most common complications affecting hospitalized patients . Intensive care unit (ICU)-acquired infections represent the majority of these infections . In a recent multicenter study conducted in 71 adult ICUs , 7.4% of the 9,493 included patients had an ICU-acquired infection. ICU-acquired pneumonia (47%) and ICU-acquired bloodstream infection (37%) were the most frequently reported infections. Another recent multicenter study was conducted in 189 ICUs . Of the 3,147 included patients, 12% had an ICU-acquired sepsis. ICU-acquired infections are frequently advocated as a significant contributor to mortality and morbidity [5, 6]. Diagnosing these infections can be difficult in ICU patients with multiorgan failure. In addition, differentiating lower respiratory tract infection from colonization can be a difficult task in patients requiring mechanical ventilation . Although mortality attributable to ICU-acquired infection is a matter of debate, high attributable morbidity and cost were repeatedly reported in patients with these infections [7–10].
Sedative and analgesic medications are routinely used in mechanically ventilated patients to reduce pain and anxiety and to allow patients to tolerate invasive procedures in the ICU . Mostly a combination of an opioid, to provide analgesia, and a hypnotic, such as a benzodiazepine or propofol to provide anxiolysis, is used . A variety of opioids used by intravenous administration in adults are available for use in the ICU, including morphine, fentanyl, alfentanil, sufentanil, and remifentanil [13–15].
Recently, several studies reported longer duration of mechanical ventilation and hospital stay in patients receiving sedation in the ICU [16, 17]. Prolonged duration of mechanical ventilation and ICU stay are well-known risk factors for ICU-acquired infection. In addition, sedation could favour infection by several other mechanisms. The aim of this review is to discuss epidemiologic data that suggest a relation between infection and sedation, to review available data for the potential causes and pathophysiology of this relation, and to identify potential preventive measures.
Materials and methods
Data for this review were identified through searches of PubMed, and from bibliographies of relevant articles. We undertook a comprehensive search in PubMed, from April 1969, through to April 2009, using the terms "infection AND sedation", "pneumonia AND sedation", "bloodstream infection AND sedation", "infection AND opioids", "infection AND hypnotics", or "infection AND opioid withdrawal" without time limit. The search was limited to publications in English and French.
Clinical studies were selected for this review if they reported on the relation between infection and sedatives used for long-term sedation in ICU patients. Animal and in vitro studies were included if they reported on the relation between infection and immunologic effects of sedation or on other potential mechanisms of infection in sedated patients. All abstracts were reviewed by two independent reviewers (SN and DeM). Articles of relevant abstracts were reviewed. All relevant articles were included in this review. After PubMed searches, 192 original articles were selected on abstracts. After reading these articles, 121 were kept in this review. Six additional original studies were found using references of selected articles.
Analgesia and sedation have routinely been employed in ICU patients for many years, particularly among those receiving mechanical ventilation. Surveys and prospective cohort studies have revealed wide variability in medication selection, monitoring using sedation scales and implementation of structured treatment algorithms among practitioners in different countries and regions of the world . However, protocols that guide the clinician to administer the least necessary sedation to achieve patient comfort while maintaining patient-examiner interactivity are recommended . In an international cohort study conducted in 1998 , 68% of the 5,183 mechanically ventilated adults received a sedative at any time while receiving mechanical ventilation. At least one analgesic or sedative drug was used on 58% of days of ventilatory support, including benzodiazepines in 69%, propofol in 21% and opioids in 63% of sedation days. Heterogeneity in clinical practice for different regions of the world was demonstrated, with use of analgesic and sedative drugs being most common in Europe and least common in Latin America. According to the results of a recent survey performed in 647 ICU physicians , substantial differences exist in sedative and analgesic practices in western European ICUs. Midazolam and propofol were the more frequently used sedatives, and morphine and fentanyl were the most frequently used analgesics. In France, a prospective, observational study was performed on 1,381 adult patients in 44 ICUs . Sedatives were used less frequently than opioids (72% and 90%, respectively), and a large proportion of assessed patients (40 to 50%) were in a deep state of sedation.
In a retrospective case-control study, opiate analgesics were found to contribute to the development of postburn infectious complications when the burn injury is of a less severe nature . With 187 controls, 187 patients with at least one infectious complication were matched according to age ± one year, length of hospital stay before infection, and total body surface area burned ± 5%. The median opiate equivalent was 14 in cases compared with 10 in controls (P = 0.06). Cases were more likely to be classified into the high opiate equivalent group relative to controls (odds ratio (OR), 1.24; 95% confidence interval (CI), 1 to 1.54; P = 0.049). The duration of opiate use was significantly longer in cases as compared with controls (P < 0.001). The association between opiate use and infection was modified by burn size. Limitations of this study included the retrospective observational design, and the absence of adjustment for comorbidities. In a large prospective observational multicenter study, an intermediate value (6 to 13) of the actual Glasgow coma scale on day 1, reflecting either preexisting disease or the effects of sedation, was significantly more frequent in patients with early-onset ventilator-associated pneumonia (VAP) compared with those without early-onset VAP (52% vs 37%, P = 0.03). In addition, a Glasgow coma scale value of 6 to 13 was independently associated with early-onset VAP (OR, 1.95; 95% CI, 1.2 to 3.18). In a prospective observational multicenter study, Metheny and colleagues determined risk factors for VAP . A high level of sedation was identified as an independent risk factor for VAP (OR, 2.3; 95% CI, 1.3 to 4.1; P = 0.006). Other risk factors included abundant aspiration (OR, 4.2; 95% CI, 2.7 to 6.7; P < 0.001), and paralytic agent use (OR, 2.7; 95% CI, 1.6 to 4.5; P < 0.001).
Another recent prospective observational study evaluated risk factors for ICU-acquired infection . Of the 587 patients, 39% developed at least one ICU-acquired infection. Although higher rates of sedation were found in patients with ICU-acquired infection compared with those without ICU-acquired infection (87% vs 53%; OR, 5.7; 95% CI, 3.7 to 8.9; P < 0.001), sedation was not independently associated with ICU-acquired infection. However, remifentanil withdrawal was identified as an independent risk factor for ICU-acquired infection (OR, 2.53; 95% CI, 1.28 to 4.19; P = 0.007). The highest rate of ICU-acquired infection was observed at day 4 after remifentanil discontinuation. However, this study was observational, and performed in a single center. Therefore, no cause-to-effect relation could be determined, and the results may not be applicable to patients hospitalized in other ICUs. Results of studies reporting on the relation between sedation and ICU-acquired infection are presented in Table 1.
The data from these epidemiologic studies suggest that there is a potential association between sedation and infection. In light of the wide and variable application of sedatives in ICU patients, where management of infection is crucial, the relation between sedative agents and infection merits further investigation.
Exposure to risk factors for ICU-acquired infection
Several studies demonstrated that sedation prolongs exposure to risk factors for ICU-acquired infection. In a prospective observational cohort study performed on 252 consecutive ICU patients requiring mechanical ventilation , Kollef and colleagues found that duration of mechanical ventilation was significantly longer for patients receiving continuous intravenous sedation compared with patients not receiving continuous intravenous sedation (185 ± 190 vs 55.6 ± 75.6 hours; P < 0.001). Similarly, the lengths of intensive care (13.5 ± 33.7 vs 4.8 ± 4.1 days; P < 0.001) and hospitalization (21.0 ± 25.1 vs 12.8 ± 14.1 days; P < 0.001) were statistically longer among patients receiving continuous intravenous sedation. In a multicenter study performed on a cohort of 5,183 patients receiving mechanical ventilation , a total of 3,540 (68%) patients received sedation. The persistent use of sedatives was associated with more days of mechanical ventilation (median, 4 (interquartile range (IQR), 2 to 8), vs 3 (2 to 4) days, P < 0.001; in patients who received sedatives, and those who did not receive sedatives; respectively); and longer length of stay in the ICU (8 (5 to 15), vs 5 (3 to 9) days, P < 0.001). Further, muscle relaxants are adjuncts to sedation in some patients. The use of muscle relaxant agents is a well-known risk factor for polyneuropathy and prolonged mechanical ventilation duration .
Duration of mechanical ventilation is a well-known risk factor for VAP. Cook and colleagues  reported that the cumulative risk of VAP increased over time, although the daily hazard risk decreased after day 5 of mechanical ventilation (3.3% at day 5, 2.3% at day 10, and 1.3% at day 15). Prolonged stay in the ICU is associated with increased exposure to invasive procedures such as intubation, and central venous, arterial and urinary catheters. Device use is the major risk factor for VAP, bloodstream infection, and urinary tract infection [3, 26, 27].
Many studies have found an association between coma as the reason for ICU admission and VAP [25, 28–31]. One potential explanation for the association between neurologic impairment and VAP is microaspiration of contaminated oropharyngeal secretions. Bacterial colonization of the aerodigestive tract and entry of contaminated secretions into the lower respiratory tract are critical in the pathogenesis of VAP . The endotracheal tube is an important risk factor for VAP, because it permits leakage of oropharyngeal secretions around the cuff and may act as a nidus for the growth of intraluminal biofilms . A recent prospective observational study aimed to determine the frequency of pepsin-positive tracheal secretions (a proxy for the aspiration of gastric contents), outcomes associated with aspiration, and risk factors for aspiration in 360 critically ill tube-fed patients . Almost 6,000 tracheal secretions collected during routine suctioning were assayed for pepsin; of these, 31.3% were positive. At least one aspiration event was identified in 88.9% (n = 320) of the participants. The incidence of pneumonia (as determined by the Clinical Pulmonary Infection Score) increased from 24% on day 1 to 48% on day 4. Patients with pneumonia on day 4 had a significantly higher percentage of pepsin-positive tracheal secretions than did those without pneumonia (42.2% vs. 21.1%, respectively; P < 0.001). Interestingly, a Glasgow Coma Scale score of less than nine (P = 0.021) was significantly associated with aspiration by univariate analysis. Other risk factors for aspiration included a low backrest elevation (P = 0.024), vomiting (P = 0.007), gastric feedings (P = 0.009), and gastroesophageal reflux disease (P = 0.033). In a 24-hour manometric study, esophageal motility was investigated in 21 adults, including 15 consecutive ventilated patients, and 6 healthy volunteers . Irrespective of the underlying disease, propulsive motility of the esophageal body was significantly reduced during any kind of sedation. Impaired tubular esophageal motility is involved in the pathogenesis of gastrointestinal reflux disease, which, in turn has been shown to cause nosocomial pneumonia in critically ill patients.
Microcirculatory effects of sedation
In a pilot study performed on 10 ICU patients, benzodiazepine induced an increase in cutaneous blood flow secondary to vasodilation, a decrease in reactive hyperemia, and alterations of vasomotion . Addition of sufentanil did not substantially modify the results obtained. Clinical studies have clearly established that alterations of normal microcirculatory control mechanisms may compromise the tissue nutrient blood flow and may contribute to the development of organ failure in septic patients [36, 37]. In addition, numerous experimental studies have reported that microvascular blood flow is altered in sepsis and common findings include a decrease in functional capillary density and heterogeneity of blood flow with perfused capillaries in close vicinity for nonperfused capillaries [38, 39]. Multiple factors may contribute to these findings, including alterations in red blood cell rheology and leucocyte adhesion to endothelial cells, endothelium dysfunction, and interstitial edema. These observations suggest that sedation may alter tissue perfusion when already compromised, as in septic patients, and contribute to the development of multiorgan failure.
Intestinal effects of sedation
Gastrointestinal motility disturbances are common in critically ill patients . These disturbances cause considerable discomfort to the patients and they are also associated with an increased rate of complications. In addition, fecal stasis induces microbiological imbalance, resulting in overgrowth of Gram-negative bacteria, relative reduction of the endogenous anaerobic and Gram-positive flora, and increase in endotoxin load. Translocation of bacteria may lead to infections, and translocation of endotoxins may enhance systemic inflammation [41–44]. Opioid drugs inhibit gastrointestinal transit by inhibiting neurotransmitter release and by changing neural excitability . An animal model demonstrated that one-quarter of the dose needed to produce analgesia inhibits intestinal motility and one-twentieth of the analgesic dose is sufficient to stop diarrhea . In contrast to many other opioid-induced side effects such as nausea, vomiting, and sedation, patients rarely develop tolerance to constipating effects of opioids . Dexmedetomidine was also found to inhibit gastric, small bowel, and colonic motility . In contrast, continuous infusion of propofol does not alter gastrointestinal tract motility more than a standard isolflurane anaesthesia .
Immunomodulatory effects of sedation
Experimental evidence coming from in vitro and in vivo animal studies suggests that opioids may alter the immunologic response to exogenous stimuli resulting in higher risk of infection. Opioids have been found to have deleterious effects on host immunity across a broad range of pathogenic microorganism [49–55]. Their immunomodulatory effects have been observed following acute and chronic exposure and after opioid withdrawal in several infectious models.
1. Acute exposure to opioids
Acute exposure to morphine suppresses mitogen-stimulated proliferation of T- and B-lymphocytes [56, 57], natural killer (NK) cell cytotoxic activity, primary antibody production [58–60], phagocytosis by macrophages [61, 62], macrophage migration via its apoptotic effects , and IL2, interferon γ (IFN), TNF-α, and nitric oxide (NO) production [64–71]. These suppressive effects are blocked by naloxone, a competitive opioid antagonist, suggesting that the effects are mediated via opioid receptors . Location of opioid receptors on immunocytes suggests that morphine suppressive effects on the immune system may be due to a direct interaction [73–76]. Another possible mechanism is that central opioid receptors activate the sympathic nervous system and the hypothalamic-pituitary-adrenal axis, which subsequently suppress immune function [77–80]. The production of cathecolamines and neuropeptides from sympathic nerves and glucocorticoids from the adrenals are responsible for many of the immunomodulatory effects of morphine . Recently, the neuroimmune mechanism of opioid-mediated conditioned immunomodulation was investigated [81–84]. Saurer and colleagues  provided evidence that the expression of morphine conditioned effects on NK cell activity requires the activation of dopamine D1 receptors in the nucleus accumbens shell. Furthermore, the antagonism of NPY Y1 receptor prevents the conditioned suppression of NK activity, suggesting that the conditioned and unconditioned effects of morphine involve similar mechanisms. Zaborina and colleagues  demonstrated that Pseudomonas aeruginosa can intercept opioid compounds released during host stress and integrate them into core elements of quorum sensing circuitry leading to enhanced virulence. These authors found that κ-opioid receptor agonists induce pyocyanin production in P. aeruginosa, and that dynorphin is released into the intestinal lumen following ischemia/reperfusion injury and accumulates in desquamated epithelium, where it binds to P. aeruginosa. Wang and colleagues  found that morphine treatment impairs TLR9-NF-κB signalling and diminishes bacterial clearance following Streptococcus pneumoniae infection in resident macrophages during the early stages of infection, leading to a compromised innate immune response. Another suggested mechanism for the immunosuppressive effects of morphine is enhancement of cellular apoptosis. In an in vitro study performed on lymphocytes infected with simian immunodeficiency virus (SIV), morphine-induced alteration in apoptotic and anti-apoptotic elements was found to be associated with accelerated viral progression . One could wonder whether the immunomodulatory effects of sedative agents could be beneficial in septic patients by damping down an uncontrolled immune response to sepsis. However, to our knowledge, no published data support this hypothesis.
2. Chronic exposure to opioids
Morphine immunopharmacological effects following chronic administration are controversial. Kumar and colleagues  reported that chronic morphine exposure caused pronounced virus replication in the cerebral compartment and accelerated onset of AIDS in SIV/SHIV-infected Indian rhesus macaques. Moreover, chronic exposure to morphine altered lipopolysaccharide (LPS)-induced inflammatory response and accelerated progression to septic shock in the rat . Martucci and colleagues  analyzed the effects of fentanyl and buprenophine on splenic cellular immune responses in the mouse. They found that opioid-induced immunosuppression was less relevant in chronic administration than in acute or short-time administration. In mice implanted with morphine pellets, concanavalin (Con) A and LPS-stimulated splenocyte proliferation is maximally suppressed at 72 hours post implantation . This suppression recovered by 96 hours independent of plasma morphine concentration, suggesting tolerance development . Another study reported tolerance to morphine-induced suppression of NK cell activity after a 14 day period of chronic morphine administration . Avila and colleagues  found that animals chronically treated with morphine became tolerant to its effects on the hypothalamic-pituitary-adrenal axis, and to its effects on T-lymphocyte proliferation. In contrast, other studies report that immune status does not recover after chronic morphine administration [60, 95, 96].
3. Opioid withdrawal
Several recent animal studies reported profound and prolonged immunosuppressive effects during the period following opioid withdrawal. Increased levels of corticosterone were observed on sudden withdrawal of morphine administration [94, 97], with return to basal levels within 72 hours. A significant suppression of lymphocyte responses was also observed within 24 hours after cessation of morphine administration. The suppression of lymphocyte proliferation was significant up to 72 hours of withdrawal of chronic morphine . A decrease in animal weight, with a peak occurring at 24 hours following withdrawal induction, and a time-dependent suppression of concalavalin A (Con-A) and toxic shock syndrome toxin (TSST)-1-stimulated splenic T-cell proliferation, Con-A-stimulated splenocyte, IFN-γ production, and splenic NK cell activity were also reported . Because clonidine inhibited these norepinephrine-dependent systems, it was suggested that opioid withdrawal-induced hyperactivity of the sympathic nervous system, and hypothalamic-pituitary-adrenal axis were responsible for these immunomodulatory effects. Abrupt morphine withdrawal, by removal of morphine pellets from dependent animals, resulted in profound immunosuppression that was maximal at 48 hours after pellet removal and was still present at 144 hours. In contrast, precipitated withdrawal, by removal of morphine pellets from dependent animals and injection of opioid antagonist, resulted in a short period of immunopotentiation at three hours after pellet removal, followed by profound immunosuppression at 24 hours post-withdrawal with a rapid return to normal immune response by 72 hours . In an in vitro model, morphine withdrawal enhances HIV infection of peripheral blood lymphocytes and T cell lines through the induction of substance P . Further, morphine withdrawal favoured hepatitis C virus (HCV) persistence in hepatic cells by suppressing IFN-α-mediated intracellular innate immunity and contributed to the development of chronic HCV infection . Other studies, performed in mice, demonstrated that morphine withdrawal was associated with increased production of TNF-α and NO, and decreased IL-12 levels [102, 103]. Feng and colleagues  showed that morphine withdrawal sensitizes to oral infection with a bacterial pathogen and predisposes mice to bacterial sepsis. Withdrawal significantly decreased the mean survival time and significantly increased the Salmonella burden in various tissues of infected mice compared with placebo-withdrawn animals. Increased bacterial colonization in this variety of tissues was observed from one day to as long as six days after withdrawal.
It was suggested that benzodiazepines bind to specific receptors on macrophages and inhibit their capacity to produce IL-1, IL-6, and TNF-α . Several studies have found that midazolam inhibits human neutrophil function and the activation of mast cells induced by TNF-α in vitro and suppresses the expression of IL-6 mRNA in blood monoclear cells . Midazolam and propofol were found to inhibit both chemotaxis and exocytosis of mast cells, whereas thiopental only inhibited chemotaxis, and ketamine only inhibited exocytosis . In utero exposure of rats to low dosages of diazepam has been found to result in depression of cellular and humoral immune responses during adulthood, with marked changes in macrophage spreading and phagocytosis. An impaired defence against Mycobacterium bovis was found in adult hamsters after in utero exposure to a dosage of 1.5 mg/kg of diazepam . These effects could be explained by a direct and/or indirect action of diazepam on the cytokine network. They could also be related to stimulation of peripheral benzodiazepine receptor binding sites (PBR) by macrophages and/or lymphocytes, or they may be mediated by PBR stimulation of the adrenals . In contrast, other investigators reported that midazolam did not alter LPS-stimulated cytokine response in vitro, or cytokine production in septic patients [110, 111].
An in vitro study tested the effects of propofol and midazolam on neutrophil function during sepsis . In both early (at 4 hours) and late (at 24 hours) sepsis, propofol and midazolam depressed hydrogen peroxide production by blood and peritoneal neutrophils at clinical concentrations. Propofol caused more depression than midazolm (P < 0.005). Further, propofol was found to improve endothelial dysfunction and to attenuate vascular superoxide production in septic rats . Propofol treatment attenuated the overproduction of NO and superoxide, thus restoring the acetylcholine-responsive NO-cyclic guanosine monophosphate (GMP) pathway in cecal ligation and puncture (CLP)-induced sepsis. It also significantly improved the CLP-impaired endothelium-dependent relaxation and endothelium-derived NO in a parallel manner. In rats with endotoxin-induced shock, treatment with propofol suppressed the release of TNF-α, IL-1β, IL-10, and NO production . In addition, in anesthetized rabbits with acute lung injury, propofol attenuated lung leucosequestration, pulmonary edema, pulmonary hyperpermeability, and resulted in better oxygenation, lung mechanics, and histologic changes . Taken together, these findings suggest that propofol administration could be beneficial in sepsis.
Clonidine and dexmedetomidine
Studies have shown that central-acting alpha-2 agonists inhibit noradrenergic neurotransmission and have a strong sedative component secondary to sympathetic inhibition . This formerly adverse side effect is widely used nowadays in critical care settings to sedate patients and to reduce the amount of co-medication needed. A recent study has shown the beneficial effects of dexmedetomidine over lorazepam as an adjunct sedative in a critical care setting . Furthermore, clonidine is an integral part of the sedation regimen in German ICUs .
Evidence that the clinically used medication clonidine has the potential to be a prophylactic option in treating sepsis has come from Kim and Hahn . They have shown that clonidine pre-medication is able to significantly reduce the pro-inflammatory cytokines IL-1β and IL-6 in patients undergoing hysterectomy.
In rats, with endotoxin-induced shock, dexmedetomidine dose-dependently attenuated extremely high mortality rates and increased plasma cytokine concentration . In addition, the early administration of dexmedetomidine drastically reduced mortality and inhibited cytokine response in endotoxin-exposed rats. Moreover, Hofer and colleagues  demonstrated that clonidine and dexmedetomidine improve survival in murine experimental sepsis. Down-regulation of pro-inflammatory mediators due to sympatholytic effects of the above mentioned drugs most probably responsible for this effect. The authors suggested that sympatholytics such as clonidine or dexmedetomidine may therefore be useful adjunct sedatives in the pre-emptive treatment of patients with a high risk for developing sepsis. However, recent studies ruled out a cholinergic interaction between the vagus nerve and the immune system . Physiologic studies understanding the neuroimmune connections can provide major advantages to design novel therapeutic strategies for sepsis .
Barbiturates are used for deep sedation in patients with elevated intracranial pressure refractory to standard therapeutic regimens. Correa-Sales and colleagues  showed that antigen-specific lymphocyte proliferation and IL-2 production by peripheral blood lymphocytes from patients under thiopental anesthesia were significantly depressed. In contrast, mitogen-induced lymphocyte proliferation, IL-2, and IL-4 secretion were not depressed. In spite of the transient decrease in antigen-driven IL-2 synthesis, no clinical evidence of infection was noted in any healthy patient. In an in vivo study, pentobarbital suppressed the expression of TNF-α mRNA and its proteins, which may result from a decrease in the activities of nuclear factor-κB and activator protein 1 and the reduction of the expression of p38 mitogen-activated protein kinase by pentobarbital . In addition, pentobarbital directly enhanced the viabilities of cells, and protected cells from apoptosis induced by deferoxamine mesylate-induced hypoxia. Further, in an in vitro model substantially different effects of barbiturates and propofol were found on phagocytosis of Staphylococcus aureus. The inhibitory effects of barbiturates demonstrated a strong dose-dependency. Impairment of phagocytosis activity was more pronounced than granulocyte recruitment.
Modulation of sedation to prevent ICU-acquired infection
Daily interruption of continuous sedation
Recently, the impact of daily interruption of continuous sedative infusions on patient outcome was evaluated by a randomized controlled trial involving 128 adult patients receiving continuous sedation and mechanical ventilation in a medical ICU . Duration of mechanical ventilation was significantly shorter in the daily interruption group compared with control group (median 4.9 vs 7.3 days, P = 0.004). Complications related to undersedation, such as removal of the endotracheal tube by the patient, were similar in the two groups. These results were confirmed by two subsequent randomized trials that paired daily interruption of sedation with ventilator weaning protocol , or early physical and occupational therapy . Several recent studies evaluated the efficacy of an expanded ventilator bundle, including daily interruption of sedation, for the reduction of VAP in ICU patients [130–135]. A significant reduction of VAP rate was found by these studies. However, many of these studies are difficult to interpret because they do not report bundle compliance rate, do not control for other specific VAP risk factors, and use the clinical definition of VAP . In addition, whether this reduction in VAP rate is related to daily interruption of sedation or to other measures used to prevent VAP, such as head-of-bed-elevation, peptic ulcer disease prophylaxis, oral care, or hand washing, is unknown.
Nurse-implemented sedation protocol
In a randomized controlled trial including 321 patients , Brook and colleagues compared a practice of protocol-directed sedation during mechanical ventilation implemented by nurses with traditional non-protocol-directed sedation administration. The median duration of mechanical ventilation was significantly shorter in patients managed with protocol-directed sedation compared with patients receiving non-protocol-directed sedation (55.9 vs 117 hours, P = 0.008). Lengths of stay in the intensive care unit (5.7 ± 5.9 vs 7.5 ± 6.5 days; P = 0.013) and hospital (14.0 ± 17.3 vs 19.9 ± 24.2 days; P < 0.001) were also significantly shorter among patients in the protocol-directed sedation group. In addition, a before-and-after prospective study found the implementation of a nursing-driven protocol of sedation to be associated improved probability of successful extubation in a heterogeneous population of mechanically ventilated patients . Another recent randomized study compared daily interruption of sedation and sedation algorithms in 74 patients under mechanical ventilation . The protocol-directed sedation group had shorter duration of mechanical ventilation (median 3.9 vs 6.7 days; P = 0.0003), faster improvement of Sequential Organ Failure Assessment over time (0.23 vs 0.7 units per day; P = 0.025), shorter ICU length of stay (8 versus 15 days; P < 0.0001), and shorter hospital length of stay (12 vs 23 days; P = 0.01). However, two recent Australian trials provided no evidence of a substantial reduction in the duration of mechanical ventilation or length of stay with the use of protocol-directed sedation compared with usual local management [140, 141]. Qualified high-intensity nurse staffing and routine Australian ICU nursing responsibility for many aspects of ventilatory practice may explain the contrast between these findings and other studies.
Quenot and colleagues  performed a prospective before-after study to determine the impact of a nurse-implemented sedation protocol on the incidence of VAP. A total of 423 patients were enrolled (control group, n = 226; protocol group, n = 197). The incidence of VAP was significantly lower in the protocol group compared with the control group (6% and 15%, respectively; P = 0.005). A nurse-implemented protocol was found to be independently associated with a lower incidence of VAP after adjustment on Simplified Acute Physiology Score II in the multivariate Cox proportional hazards model (hazard rate, 0.81; 95% CI, 0.62 to 0.95; P = 0.03). The median duration of mechanical ventilation was significantly shorter in the protocol group compared with the control group (4.2 vs 8 days; P = 0.001). Potential means to reduce ICU-acquired infection in sedated patients are presented in Table 4.
Comparison of sedative agents
In a prospective randomized pilot study, the influence of fentanyl-based versus remifentanil-based anesthesia on cytokine responses and expression of the suppressor of cytokine signalling (SOCS)-3 gene was compared in 40 patients following coronary artery bypass graft surgery . The IFN-γ/IL-10 ratio after Con-A stimulation in whole blood cells on post-operative day 1, and SOCS-3 gene expression on post-operative day 2 were significantly lower in the remifentanil group than in the fentanyl group. The time in the ICU was also significantly lower in the remifentanil group. These findings suggest that remifentanil can attenuate the exaggerated inflammatory response that occurs after cardiac surgery with cardiopulmonary bypass. Two recent randomized controlled studies found a remifentanil/propofol-based sedation regimen to be associated with shorter duration of mechanical ventilation and ICU stay compared with a conventional regimen [14, 15].
In a double-blind randomized placebo-controlled trial performed in 33 newborn babies, sedation provided by continuous infusion of midazolam and morphine was comparable to morphine alone, with no significant adverse effects . Interestingly, infection rate was similar in the two groups. The effects of prolonged infusion of midazolam and propofol on immune function were compared in a randomized study including 40 critically ill surgical patients who were to receive long-term sedation for more than two days . Although midazolam suppressed the production of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α, both agents caused suppression of IL-8 production. Propofol inhibited IL-2 production and stimulated IFN-γ production, whereas midazolam failed to do so. Kress and colleagues  compared propofol and midazolam in a randomized study involving 73 patients (37 in propofol group and 36 in midazolam group). The propofol group had a significantly narrower range of wake-up times with a higher likelihood of waking in less than 60 minutes.
An observational study found patients with withdrawal syndrome to have significantly elevated hemodynamic, metabolic, and respiratory demands . Clonidine significantly decreased these demands, induced mild sedation, and facilitated patient cooperation with the ventilator, enabling ventilator weaning. A recent prospective randomized study compared the effects of dexmedetomidine or midazolam infusion together with an alfentanil infusion for analgesia if required on the inflammatory responses and gastric intramucosal pH in critically ill patients . Fourty patients were included, and there was no statistically significant differences between the groups with respect to hemodynamic and biochemical measurements, or gastric intramucosal pH. However, there were significant decreases in TNF-α, IL-1β, IL-6 at 24 hours in the dexmedetomidine group compared with the midazolam group. Another recent prospective double-blind randomized study compared the efficacy and safety of prolonged sedation with dexmedetomidine and midazolam among 375 mechanically ventilated patients . Infection rate was significantly lower in the dexmedetomidine group compared with the midazolam group (10.2 vs 19.7%, P = 0.02). Although length of ICU stay was similar in the two groups, median time to extubation was significantly shorter in the dexmedetomidine group compared with the midazolam group (3.7 vs 5.6 days, P = 0.01).
A retrospective study compared the rate of pneumonia between ventilated head trauma patients who received thiopental therapy (n = 75) and those who did not receive thiopental (n = 76) . The rate of noscomial pneumonia was higher in patients who received thiopental compared with those who did not receive thiopental (53 vs 35%; OR, 1.85; 95% CI, 0.97 to 3.51). In addition, thiopental therapy was independently associated with nosocomial pneumonia. Results of studies comparing different sedative agents with regard to cytokine levels, infection rate and other outcomes are presented in Table 5.
Our review has some limitations. First, there is strong evidence coming from animal studies that sedative agents could alter immune function and increase the risk of infection. However, clinical studies are needed to determine whether these data are relevant in the clinical setting. The epidemiologic studies showed a link between sedation and infection. However, no cause-to-effect relation could be demonstrated. Second, the subject of our review is vast and the literature covering the effects of sedative agents on immune function is very large. Therefore, this could not be a comprehensive review of the total literature on this subject within the size of the article. Third, some sedative agents used for short sedation, such as etomidate, were not reviewed. In addition, effects of muscle relaxants on infection were not reviewed.
Future studies should compare the effect of different sedative agents on the incidence of ICU-acquired infection. Further, the impact of progressive opioid discontinuation on the risk of ICU-acquired infection should be compared with abrupt discontinuation. The role of intermittent dosing rather than infusion of sedative agents should also be evaluated. The impact of adjunctive agents, such as clonidine, should be evaluated. In addition, analgesics other than opioids should be explored in ICU patients, and the risk of ICU-acquired infections should be compared between opioids and other analgesics. Volatile sedation using isoflurane appears a promising alternative to intravenous sedatives for adult patients mechanically ventilated in the ICU. Finally, peripherally acting mu-opioid receptor antagonists methylnatrexone and alvimopan are a new class of drugs designed to reverse opioid-induced side effects on the gastrointestinal system without compromising pain relief . A recent randomized controlled study demonstrated that methylnatrexone rapidly induced laxation in patients with advanced illness and opioid-induced constipation . Treatment did not appear to affect central analgesia or precipitate opioid withdrawal. Future studies should determine whether these results are applicable in ICU patients, and whether treatment with these antagonists could influence gastrointestinal translocation and ICU-acquired infections.
Sedation is associated with increased risk of ICU-acquired infection. Prolongation of exposure to risk factors for infection, microaspiration, gastrointestinal motility disturbances, microcirculatory effects, and immunomodulatory effects are the main mechanisms by which sedation might favor infection in critically ill patients. Clinical studies comparing different sedative agents do not provide evidence to recommend the use of a particular agent to reduce ICU-acquired infection rate. However, sedation strategies aiming to reduce the duration of mechanical ventilation, such as daily interruption of sedatives or nursing-implementing sedation protocol, should be promoted. In addition, the use of short-acting opioids, propofol, and dexmedetomidine is associated with shorter duration of mechanical ventilation and ICU stay, and might be helpful in reducing ICU-acquired infection rates.
Several epidemiologic studies suggest a link between sedation and ICU-acquired infection.
Prolongation of exposure to risk factors for infection, microaspiration, gastrointestinal motility disturbances, microcirculatory effects and immunomodulatory effects are main mechanisms by which sedation may favor infection in critically ill patients.
Clinical studies comparing different sedative agents do not provide evidence to recommend the use of a particular agent to reduce ICU-acquired infection rate.
Sedation strategies aiming to reduce the duration of mechanical ventilation, such as daily interruption of sedatives or nursing-implementing sedation protocol, should be promoted.
The use of short-acting opioids, propofol, and dexmedetomidine is associated with shorter duration of mechanical ventilation and ICU stay, and might be helpful in preventing ICU-acquired infections.
cecal ligation and puncture
hepatitis C virus
intensive care unit
peripheral benzodiazepines receptor
simian immunodeficiency virus
suppressor of cytokine signalling
tumor necrosis factor
toxic shock syndrome toxin
Burke JP: Infection control - a problem for patient safety. N Engl J Med 2003, 348: 651-656. 10.1056/NEJMhpr020557
Hugonnet S, Uckay I, Pittet D: Staffing level: a determinant of late-onset ventilator-associated pneumonia. Crit Care 2007, 11: R80. 10.1186/cc5974
Malacarne P, Langer M, Nascimben E, Moro ML, Giudici D, Lampati L, Bertolini G: Building a continuous multicenter infection surveillance system in the intensive care unit: findings from the initial data set of 9,493 patients from 71 Italian intensive care units. Crit Care Med 2008, 36: 1105-1113. 10.1097/CCM.0b013e318169ed30
Vincent JL, Sakr Y, Sprung CL, Ranieri VM, Reinhart K, Gerlach H, Moreno R, Carlet J, Le Gall JR, Payen D: Sepsis in European intensive care units: results of the SOAP study. Crit Care Med 2006, 34: 344-353. 10.1097/01.CCM.0000194725.48928.3A
Nseir S, Di Pompeo C, Soubrier S, Cavestri B, Jozefowicz E, Saulnier F, Durocher A: Impact of ventilator-associated pneumonia on outcome in patients with COPD. Chest 2005, 128: 1650-1656. 10.1378/chest.128.3.1650
Safdar N, Abad C: Educational interventions for prevention of healthcare-associated infection: a systematic review. Crit Care Med 2008, 36: 933-940. 10.1097/CCM.0B013E318165FAF3
Tacconelli E, De Angelis G: Pneumonia due to methicillin-resistant Staphylococcus aureus: clinical features, diagnosis and management. Curr Opin Pulm Med 2009, 15: 218-222. 10.1097/MCP.0b013e3283292666
Guidelines for the management of adults with hospital-acquired, ventilator-associated, and healthcare-associated pneumonia Am J Respir Crit Care Med 2005, 171: 388-416. 10.1164/rccm.200405-644ST
Safdar N, Dezfulian C, Collard HR, Saint S: Clinical and economic consequences of ventilator-associated pneumonia: a systematic review. Crit Care Med 2005, 33: 2184-2193. 10.1097/01.CCM.0000181731.53912.D9
Ylipalosaari P, Ala-Kokko TI, Laurila J, Ohtonen P, Syrjala H: Intensive care unit acquired infection has no impact on long-term survival or quality of life: a prospective cohort study. Crit Care 2007, 11: R35. 10.1186/cc5718
Sessler CN, Varney K: Patient-focused sedation and analgesia in the ICU. Chest 2008, 133: 552-565. 10.1378/chest.07-2026
Richman PS, Baram D, Varela M, Glass PS: Sedation during mechanical ventilation: a trial of benzodiazepine and opiate in combination. Crit Care Med 2006, 34: 1395-1401. 10.1097/01.CCM.0000215454.50964.F8
Mehta S, Burry L, Fischer S, Martinez-Motta JC, Hallett D, Bowman D, Wong C, Meade MO, Stewart TE, Cook DJ: Canadian survey of the use of sedatives, analgesics, and neuromuscular blocking agents in critically ill patients. Crit Care Med 2006, 34: 374-380. 10.1097/01.CCM.0000196830.61965.F1
Muellejans B, Matthey T, Scholpp J, Schill M: Sedation in the intensive care unit with remifentanil/propofol versus midazolam/fentanyl: a randomised, open-label, pharmacoeconomic trial. Crit Care 2006, 10: R91. 10.1186/cc4939
Rozendaal FW, Spronk PE, Snellen FF, Schoen A, van Zanten AR, Foudraine NA, Mulder PG, Bakker J: Remifentanil-propofol analgo-sedation shortens duration of ventilation and length of ICU stay compared to a conventional regimen: a centre randomised, cross-over, open-label study in the Netherlands. Intensive Care Med 2009, 35: 291-298. 10.1007/s00134-008-1328-9
Kollef MH, Levy NT, Ahrens TS, Schaiff R, Prentice D, Sherman G: The use of continuous i.v. sedation is associated with prolongation of mechanical ventilation. Chest 1998, 114: 541-548. 10.1378/chest.114.2.541
Arroliga A, Frutos-Vivar F, Hall J, Esteban A, Apezteguia C, Soto L, Anzueto A: Use of sedatives and neuromuscular blockers in a cohort of patients receiving mechanical ventilation. Chest 2005, 128: 496-506. 10.1378/chest.128.2.496
Sessler CN, Wilhelm W: Analgesia and sedation in the intensive care unit: an overview of the issues. Crit Care 2008,12(Suppl 3):S1.
Schweickert WD, Kress JP: Strategies to optimize analgesia and sedation. Crit Care 2008,12(Suppl 3):S6.
Soliman HM, Melot C, Vincent JL: Sedative and analgesic practice in the intensive care unit: the results of a European survey. Br J Anaesth 2001, 87: 186-192. 10.1093/bja/87.2.186
Payen JF, Chanques G, Mantz J, Hercule C, Auriant I, Leguillou JL, Binhas M, Genty C, Rolland C, Bosson JL: Current practices in sedation and analgesia for mechanically ventilated critically ill patients: a prospective multicenter patient-based study. Anesthesiology 2007, 106: 687-695. 10.1097/01.anes.0000264747.09017.da
Schwacha MG, McGwin G Jr, Hutchinson CB, Cross JM, Maclennan PA, Rue LW: The contribution of opiate analgesics to the development of infectious complications in burn patients. Am J Surg 2006, 192: 82-86. 10.1016/j.amjsurg.2006.01.001
Metheny NA, Clouse RE, Chang YH, Stewart BJ, Oliver DA, Kollef MH: Tracheobronchial aspiration of gastric contents in critically ill tube-fed patients: frequency, outcomes, and risk factors. Crit Care Med 2006, 34: 1007-1015. 10.1097/01.CCM.0000206106.65220.59
Nseir S, Hoel J, Grailles G, Soury-Lavergne A, Di Pompeo C, Mathieu D, Durocher A: Remifentanil discontinuation and subsequent intensive care unit-acquired infection: a cohort study. Crit Care 2009, 13: R60. 10.1186/cc7788
Cook DJ, Walter SD, Cook RJ, Griffith LE, Guyatt GH, Leasa D, Jaeschke RZ, Brun-Buisson C: Incidence of and risk factors for ventilator-associated pneumonia in critically ill patients. Ann Intern Med 1998, 129: 433-440.
Kooi TI, de Boer AS, Mannien J, Wille JC, Beaumont MT, Mooi BW, van den HS: Incidence and risk factors of device-associated infections and associated mortality at the intensive care in the Dutch surveillance system. Intensive Care Med 2007, 33: 271-278. 10.1007/s00134-006-0464-3
Hugonnet S, Chevrolet JC, Pittet D: The effect of workload on infection risk in critically ill patients. Crit Care Med 2007, 35: 76-81. 10.1097/01.CCM.0000251125.08629.3F
Akca O, Koltka K, Uzel S, Cakar N, Pembeci K, Sayan MA, Tutuncu AS, Karakas SE, Calangu S, Ozkan T, Esen F, Telci L, Sessler DI, Akpir K: Risk factors for early-onset, ventilator-associated pneumonia in critical care patients: selected multiresistant versus nonresistant bacteria. Anesthesiology 2000, 93: 638-645. 10.1097/00000542-200009000-00011
Bornstain C, Azoulay E, De Lassence A, Cohen Y, Costa MA, Mourvillier B, Descorps-Declere A, Garrouste-Org , Thuong M, Schlemmer B, Timsit JF: Sedation, sucralfate, and antibiotic use are potential means for protection against early-onset ventilator-associated pneumonia. Clin Infect Dis 2004, 38: 1401-1408. 10.1086/386321
Nseir S, Di Pompeo C, Jozefowicz E, Cavestri B, Brisson H, Nyunga M, Soubrier S, Durocher A: Relationship between tracheotomy and ventilator-associated pneumonia: a case control study. Eur Respir J 2007, 30: 314-320. 10.1183/09031936.06.00024906
Alp E, Guven M, Yildiz O, Aygen B, Voss A, Doganay M: Incidence, risk factors and mortality of nosocomial pneumonia in intensive care units: a prospective study. Ann Clin Microbiol Antimicrob 2004, 3: 17. 10.1186/1476-0711-3-17
Craven DE: Preventing ventilator-associated pneumonia in adults: sowing seeds of change. Chest 2006, 130: 251-260. 10.1378/chest.130.1.251
Safdar N, Crnich CJ, Maki DG: The pathogenesis of ventilator-associated pneumonia: its relevance to developing effective strategies for prevention. Respir Care 2005, 50: 725-739.
Kolbel CB, Rippel K, Klar H, Singer MV, van Ackern K, Fiedler F: Esophageal motility disorders in critically ill patients: a 24-hour manometric study. Intensive Care Med 2000, 26: 1421-1427. 10.1007/s001340000630
Lamblin V, Favory R, Boulo M, Mathieu D: Microcirculatory alterations induced by sedation in intensive care patients. Effects of midazolam alone and in association with sufentanil. Crit Care 2006, 10: R176. 10.1186/cc5128
De Backer D, Creteur J, Preiser JC, Dubois MJ, Vincent JL: Microvascular blood flow is altered in patients with sepsis. Am J Respir Crit Care Med 2002, 166: 98-104. 10.1164/rccm.200109-016OC
Sakr Y, Dubois MJ, De Backer D, Creteur J, Vincent JL: Persistent microcirculatory alterations are associated with organ failure and death in patients with septic shock. Crit Care Med 2004, 32: 1825-1831. 10.1097/01.CCM.0000138558.16257.3F
Tsai AG, Cabrales P, Winslow RM, Intaglietta M: Microvascular oxygen distribution in awake hamster window chamber model during hyperoxia. Am J Physiol Heart Circ Physiol 2003, 285: H1537-H1545.
Neviere R, Mathieu D, Chagnon JL, Lebleu N, Millien JP, Wattel F: Skeletal muscle microvascular blood flow and oxygen transport in patients with severe sepsis. Am J Respir Crit Care Med 1996, 153: 191-195.
Fruhwald S, Holzer P, Metzler H: Intestinal motility disturbances in intensive care patients pathogenesis and clinical impact. Intensive Care Med 2007, 33: 36-44. 10.1007/s00134-006-0452-7
Spoel JI, Oudemans-van Straaten HM, Kuiper MA, van Roon EN, Zandstra DF, Voort PH: Laxation of critically ill patients with lactulose or polyethylene glycol: a two-center randomized, double-blind, placebo-controlled trial. Crit Care Med 2007, 35: 2726-2731. 10.1097/01.CCM.0000287526.08794.29
Husebye E: Gastrointestinal motility disorders and bacterial overgrowth. J Intern Med 1995, 237: 419-427. 10.1111/j.1365-2796.1995.tb01196.x
Haglund U: Systemic mediators released from the gut in critical illness. Crit Care Med 1993, 21: S15-S18.
Ritz MA, Fraser R, Tam W, Dent J: Impacts and patterns of disturbed gastrointestinal function in critically ill patients. Am J Gastroenterol 2000, 95: 3044-3052. 10.1111/j.1572-0241.2000.03176.x
Sternini C, Patierno S, Selmer IS, Kirchgessner A: The opioid system in the gastrointestinal tract. Neurogastroenterol Motil 2004,16(Suppl 2):3-16. 10.1111/j.1743-3150.2004.00553.x
Walsh TD: Prevention of opioid side effects. J Pain Symptom Manage 1990, 5: 362-367. 10.1016/0885-3924(90)90031-E
Herbert MK, Roth-Goldbrunner S, Holzer P, Roewer N: Clonidine and dexmedetomidine potently inhibit peristalsis in the Guinea pig ileum in vitro. Anesthesiology 2002, 97: 1491-1499. 10.1097/00000542-200212000-00022
Freye E, Sundermann S, Wilder-Smith OH: No inhibition of gastro-intestinal propulsion after propofol- or propofol/ketamine-N2O/O2 anaesthesia. A comparison of gastro-caecal transit after isoflurane anaesthesia. Acta Anaesthesiol Scand 1998, 42: 664-669. 10.1111/j.1399-6576.1998.tb05299.x
Asakura H, Kawamoto K, Igimi S, Yamamoto S, Makino S: Enhancement of mice susceptibility to infection with Listeria monocytogenes by the treatment of morphine. Microbiol Immunol 2006, 50: 543-547.
Wang J, Barke RA, Charboneau R, Roy S: Morphine impairs host innate immune response and increases susceptibility to Streptococcus pneumoniae lung infection. J Immunol 2005, 174: 426-434.
Hilburger ME, Adler MW, Truant AL, Meissler JJ Jr, Satishchandran V, Rogers TJ, Eisenstein TK: Morphine induces sepsis in mice. J Infect Dis 1997, 176: 183-188. 10.1086/514021
Olin MR, Choi K, Lee J, Peterson PK, Molitor TW: Morphine modulates gammadelta lymphocytes cytolytic activity following BCG vaccination. Brain Behav Immun 2007, 21: 195-201. 10.1016/j.bbi.2006.06.003
Chao CC, Sharp BM, Pomeroy C, Filice GA, Peterson PK: Lethality of morphine in mice infected with Toxoplasma gondii. J Pharmacol Exp Ther 1990, 252: 605-609.
Di Francesco P, Gaziano R, Casalinuovo IA, Palamara AT, Favalli C, Garaci E: Antifungal and immunoadjuvant properties of fluconazole in mice immunosuppressed with morphine. Chemotherapy 1997, 43: 198-203. 10.1159/000239561
Hu S, Sheng WS, Lokensgard JR, Peterson PK: Morphine potentiates HIV-1 gp120-induced neuronal apoptosis. J Infect Dis 2005, 191: 886-889. 10.1086/427830
Bayer BM, Daussin S, Hernandez M, Irvin L: Morphine inhibition of lymphocyte activity is mediated by an opioid dependent mechanism. Neuropharmacology 1990, 29: 369-374. 10.1016/0028-3908(90)90096-A
Bayer BM, Gastonguay MR, Hernandez MC: Distinction between the in vitro and in vivo inhibitory effects of morphine on lymphocyte proliferation based on agonist sensitivity and naltrexone reversibility. Immunopharmacology 1992, 23: 117-124. 10.1016/0162-3109(92)90035-B
Shavit Y, Lewis JW, Terman GW, Gale RP, Liebeskind JC: Opioid peptides mediate the suppressive effect of stress on natural killer cell cytotoxicity. Science 1984, 223: 188-190. 10.1126/science.6691146
Bussiere JL, Adler MW, Rogers TJ, Eisenstein TK: Differential effects of morphine and naltrexone on the antibody response in various mouse strains. Immunopharmacol Immunotoxicol 1992, 14: 657-673. 10.3109/08923979209005416
West JP, Lysle DT, Dykstra LA: Tolerance development to morphine-induced alterations of immune status. Drug Alcohol Depend 1997, 46: 147-157. 10.1016/S0376-8716(97)00059-8
Tubaro E, Borelli G, Croce C, Cavallo G, Santiangeli C: Effect of morphine on resistance to infection. J Infect Dis 1983, 148: 656-666.
Rojavin M, Szabo I, Bussiere JL, Rogers TJ, Adler MW, Eisenstein TK: Morphine treatment in vitro or in vivo decreases phagocytic functions of murine macrophages. Life Sci 1993, 53: 997-1006. 10.1016/0024-3205(93)90122-J
Malik AA, Radhakrishnan N, Reddy K, Smith AD, Singhal PC: Morphine-induced macrophage apoptosis modulates migration of macrophages: use of in vitro model of urinary tract infection. J Endourol 2002, 16: 605-610. 10.1089/089277902320913314
Lysle DT, Coussons ME, Watts VJ, Bennett EH, Dykstra LA: Morphine-induced alterations of immune status: dose dependency, compartment specificity and antagonism by naltrexone. J Pharmacol Exp Ther 1993, 265: 1071-1078.
Gomez-Flores R, Weber RJ: Inhibition of interleukin-2 production and downregulation of IL-2 and transferrin receptors on rat splenic lymphocytes following PAG morphine administration: a role in natural killer and T cell suppression. J Interferon Cytokine Res 1999, 19: 625-630. 10.1089/107999099313767
Gomez-Flores R, Suo JL, Weber RJ: Suppression of splenic macrophage functions following acute morphine action in the rat mesencephalon periaqueductal gray. Brain Behav Immun 1999, 13: 212-224. 10.1006/brbi.1999.0563
Nelson CJ, How T, Lysle DT: Enhancement of the contact hypersensitivity reaction by acute morphine administration at the elicitation phase. Clin Immunol 1999, 93: 176-183. 10.1006/clim.1999.4783
Singh PP, Singal P: Morphine-induced neuroimmunomodulation in murine visceral leishmaniasis: the role(s) of cytokines and nitric oxide. J Neuroimmune Pharmacol 2007, 2: 338-351. 10.1007/s11481-007-9094-y
Jamali A, Bamdad T, Soleimanjahi H, Pakdel FG, Arefian E: Acute morphine administration reduces white blood cells' capability to induce innate resistance against HSV-1 infection in BALB/c mice. Neuroimmunomodulation 2007, 14: 16-23. 10.1159/000107284
Li Y, Ye L, Peng JS, Wang CQ, Luo GX, Zhang T, Wan Q, Ho WZ: Morphine inhibits intrahepatic interferon- alpha expression and enhances complete hepatitis C virus replication. J Infect Dis 2007, 196: 719-730. 10.1086/520093
Brand JM, Frohn C, Luhm J, Kirchner H, Schmucker P: Early alterations in the number of circulating lymphocyte subpopulations and enhanced proinflammatory immune response during opioid-based general anesthesia. Shock 2003, 20: 213-217. 10.1097/00024382-200309000-00003
Chao CC, Gekker G, Sheng WS, Hu S, Tsang M, Peterson PK: Priming effect of morphine on the production of tumor necrosis factor-alpha by microglia: implications in respiratory burst activity and human immunodeficiency virus-1 expression. J Pharmacol Exp Ther 1994, 269: 198-203.
Carr DJ: The role of endogenous opioids and their receptors in the immune system. Proc Soc Exp Biol Med 1991, 198: 710-720.
Caroleo MC, Arbitrio M, Melchiorri D, Nistico G: A reappraisal of the role of the various opioid receptor subtypes in cell-mediated immunity. Neuroimmunomodulation 1994, 1: 141-147. 10.1159/000097148
Bidlack JM: Detection and function of opioid receptors on cells from the immune system. Clin Diagn Lab Immunol 2000, 7: 719-723.
McCarthy L, Wetzel M, Sliker JK, Eisenstein TK, Rogers TJ: Opioids, opioid receptors, and the immune response. Drug Alcohol Depend 2001, 62: 111-123. 10.1016/S0376-8716(00)00181-2
Fecho K, Maslonek KA, Dykstra LA, Lysle DT: Alterations of immune status induced by the sympathetic nervous system: immunomodulatory effects of DMPP alone and in combination with morphine. Brain Behav Immun 1993, 7: 253-270. 10.1006/brbi.1993.1026
Fecho K, Dykstra LA, Lysle DT: Evidence for beta adrenergic receptor involvement in the immunomodulatory effects of morphine. J Pharmacol Exp Ther 1993, 265: 1079-1087.
Hernandez MC, Flores LR, Bayer BM: Immunosuppression by morphine is mediated by central pathways. J Pharmacol Exp Ther 1993, 267: 1336-1341.
Mellon RD, Bayer BM: Evidence for central opioid receptors in the immunomodulatory effects of morphine: review of potential mechanism(s) of action. J Neuroimmunol 1998, 83: 19-28. 10.1016/S0165-5728(97)00217-8
Saurer TB, Ijames SG, Lysle DT: Neuropeptide Y Y1 receptors mediate morphine-induced reductions of natural killer cell activity. J Neuroimmunol 2006, 177: 18-26. 10.1016/j.jneuroim.2006.05.002
Saurer TB, Carrigan KA, Ijames SG, Lysle DT: Suppression of natural killer cell activity by morphine is mediated by the nucleus accumbens shell. J Neuroimmunol 2006, 173: 3-11. 10.1016/j.jneuroim.2005.11.009
Saurer TB, Ijames SG, Carrigan KA, Lysle DT: Neuroimmune mechanisms of opioid-mediated conditioned immunomodulation. Brain Behav Immun 2008, 22: 89-97. 10.1016/j.bbi.2007.06.009
Chadzinska M, Hermsen T, Savelkoul HF, Verburg-van Kemenade BM: Cloning of opioid receptors in common carp (Cyprinus carpio L.) and their involvement in regulation of stress and immune response. Brain Behav Immun 2009, 23: 257-266. 10.1016/j.bbi.2008.10.003
Zaborina O, Lepine F, Xiao G, Valuckaite V, Chen Y, Li T, Ciancio M, Zaborin A, Petrof EO, Turner JR, Rahme LG, Chang E, Alverdy JC: Dynorphin activates quorum sensing quinolone signaling in Pseudomonas aeruginosa. PLoS Pathog 2007, 3: e35. 10.1371/journal.ppat.0030035
Wang J, Barke RA, Charboneau R, Schwendener R, Roy S: Morphine induces defects in early response of alveolar macrophages to Streptococcus pneumoniae by modulating TLR9-NF-kappa B signaling. J Immunol 2008, 180: 3594-3600.
Xu J, Li PF, Liu XH, Li G: Morphine aggravates the apoptosis of simian immunodeficiency virus infected CEM x174 cells in the prolonged culture in vitro. Int Immunopharmacol 2004, 4: 1805-1816. 10.1016/j.intimp.2004.07.025
Kumar R, Orsoni S, Norman L, Verma AS, Tirado G, Giavedoni LD, Staprans S, Miller GM, Buch SJ, Kumar A: Chronic morphine exposure causes pronounced virus replication in cerebral compartment and accelerated onset of AIDS in SIV/SHIV-infected Indian rhesus macaques. Virology 2006, 354: 192-206. 10.1016/j.virol.2006.06.020
Ocasio FM, Jiang Y, House SD, Chang SL: Chronic morphine accelerates the progression of lipopolysaccharide-induced sepsis to septic shock. J Neuroimmunol 2004, 149: 90-100. 10.1016/j.jneuroim.2003.12.016
Martucci C, Panerai AE, Sacerdote P: Chronic fentanyl or buprenorphine infusion in the mouse: similar analgesic profile but different effects on immune responses. Pain 2004, 110: 385-392. 10.1016/j.pain.2004.04.020
Bryant HU, Bernton EW, Holaday JW: Morphine pellet-induced immunomodulation in mice: temporal relationships. J Pharmacol Exp Ther 1988, 245: 913-920.
Bryant HU, Yoburn BC, Inturrisi CE, Bernton EW, Holaday JW: Morphine-induced immunomodulation is not related to serum morphine concentrations. Eur J Pharmacol 1988, 149: 165-169. 10.1016/0014-2999(88)90057-X
Shavit Y, Terman GW, Lewis JW, Zane CJ, Gale RP, Liebeskind JC: Effects of footshock stress and morphine on natural killer lymphocytes in rats: studies of tolerance and cross-tolerance. Brain Res 1986, 372: 382-385. 10.1016/0006-8993(86)91149-2
Avila AH, Alonzo NC, Bayer BM: Immune cell activity during the initial stages of withdrawal from chronic exposure to cocaine or morphine. J Neuroimmunol 2004, 147: 109-113. 10.1016/j.jneuroim.2003.10.023
Pacifici R, Di Carlo S, Bacosi A, Zuccaro P: Macrophage functions in drugs of abuse-treated mice. Int J Immunopharmacol 1993, 15: 711-716. 10.1016/0192-0561(93)90143-M
Risdahl JM, Peterson PK, Chao CC, Pijoan C, Molitor TW: Effects of morphine dependence on the pathogenesis of swine herpesvirus infection. J Infect Dis 1993, 167: 1281-1287.
Kishioka S, Nishida S, Fukunaga Y, Yamamoto H: Quantitative properties of plasma corticosterone elevation induced by naloxone-precipitated withdrawal in morphine-dependent rats. Jpn J Pharmacol 1994, 66: 257-263. 10.1254/jjp.66.257
West JP, Dykstra LA, Lysle DT: Immunomodulatory effects of morphine withdrawal in the rat are time dependent and reversible by clonidine. Psychopharmacology (Berl) 1999, 146: 320-327. 10.1007/s002130051123
Rahim RT, Adler MW, Meissler JJ Jr, Cowan A, Rogers TJ, Geller EB, Eisenstein TK: Abrupt or precipitated withdrawal from morphine induces immunosuppression. J Neuroimmunol 2002, 127: 88-95. 10.1016/S0165-5728(02)00103-0
Wang X, Douglas SD, Peng JS, Zhou DJ, Wan Q, Ho WZ: An in vitro model of morphine withdrawal manifests the enhancing effect on human immunodeficiency virus infection of human T lymphocytes through the induction of substance P. Am J Pathol 2006, 169: 1663-1670. 10.2353/ajpath.2006.060358
Wang CQ, Li Y, Douglas SD, Wang X, Metzger DS, Zhang T, Ho WZ: Morphine withdrawal enhances hepatitis C virus replicon expression. Am J Pathol 2005, 167: 1333-1340.
Feng P, Meissler JJ Jr, Adler MW, Eisenstein TK: Morphine withdrawal sensitizes mice to lipopolysaccharide: elevated TNF-alpha and nitric oxide with decreased IL-12. J Neuroimmunol 2005, 164: 57-65. 10.1016/j.jneuroim.2005.03.017
Feng P, Wilson QM, Meissler JJ Jr, Adler MW, Eisenstein TK: Increased sensitivity to Salmonella enterica serovar Typhimurium infection in mice undergoing withdrawal from morphine is associated with suppression of interleukin-12. Infect Immun 2005, 73: 7953-7959. 10.1128/IAI.73.12.7953-7959.2005
Feng P, Truant AL, Meissler JJ Jr, Gaughan JP, Adler MW, Eisenstein TK: Morphine withdrawal lowers host defense to enteric bacteria: spontaneous sepsis and increased sensitivity to oral Salmonella enterica serovar Typhimurium infection. Infect Immun 2006, 74: 5221-5226. 10.1128/IAI.00208-06
Zavala F, Taupin V, Descamps-Latscha B: In vivo treatment with benzodiazepines inhibits murine phagocyte oxidative metabolism and production of interleukin 1, tumor necrosis factor and interleukin-6. J Pharmacol Exp Ther 1990, 255: 442-450.
Nishina K, Akamatsu H, Mikawa K, Shiga M, Maekawa N, Obara H, Niwa Y: The inhibitory effects of thiopental, midazolam, and ketamine on human neutrophil functions. Anesth Analg 1998, 86: 159-165. 10.1097/00000539-199801000-00032
Fujimoto T, Nishiyama T, Hanaoka K: Inhibitory effects of intravenous anesthetics on mast cell function. Anesth Analg 2005, 101: 1054-1059. 10.1213/01.ane.0000166955.97368.80
Ugaz EM, Pinheiro SR, Guerra JL, Palermo-Neto J: Effects of prenatal diazepam treatment on Mycobacterium bovis-induced infection in hamsters. Immunopharmacology 1999, 41: 209-217. 10.1016/S0162-3109(99)00031-4
Righi DA, Pinheiro SR, Guerra JL, Palermo-Neto J: Effects of diazepam on Mycobacterium bovis-induced infection in hamsters. Braz J Med Biol Res 1999, 32: 1145-1153. 10.1590/S0100-879X1999000900014
Takaono M, Yogosawa T, Okawa-Takatsuji M, Aotsuka S: Effects of intravenous anesthetics on interleukin (IL)-6 and IL-10 production by lipopolysaccharide-stimulated mononuclear cells from healthy volunteers. Acta Anaesthesiol Scand 2002, 46: 176-179. 10.1034/j.1399-6576.2002.460209.x
Memis D, Hekimoglu S, Vatan I, Yandim T, Yuksel M, Sut N: Effects of midazolam and dexmedetomidine on inflammatory responses and gastric intramucosal pH to sepsis, in critically ill patients. Br J Anaesth 2007, 98: 550-552. 10.1093/bja/aem017
Inada T, Taniuchi S, Shingu K, Kobayashi Y, Fujisawa J, Nakao S: Propofol depressed neutrophil hydrogen peroxide production more than midazolam, whereas adhesion molecule expression was minimally affected by both anesthetics in rats with abdominal sepsis. Anesth Analg 2001, 92: 437-441. 10.1097/00000539-200102000-00030
Yu HP, Lui PW, Hwang TL, Yen CH, Lau YT: Propofol improves endothelial dysfunction and attenuates vascular superoxide production in septic rats. Crit Care Med 2006, 34: 453-460. 10.1097/01.CCM.0000198530.68343.21
Hsu BG, Yang FL, Lee RP, Peng TC, Chen HI: Effects of post-treatment with low-dose propofol on inflammatory responses to lipopolysaccharide-induced shock in conscious rats. Clin Exp Pharmacol Physiol 2005, 32: 24-29. 10.1111/j.1440-1681.2005.04155.x
Takao Y, Mikawa K, Nishina K, Obara H: Attenuation of acute lung injury with propofol in endotoxemia. Anesth Analg 2005, 100: 810-816. 10.1213/01.ANE.0000144775.19385.8C
Knaus AE, Muthig V, Schickinger S, Moura E, Beetz N, Gilsbach R, Hein L: Alpha2-adrenoceptor subtypes--unexpected functions for receptors and ligands derived from gene-targeted mouse models. Neurochem Int 2007, 51: 277-281. 10.1016/j.neuint.2007.06.036
Pandharipande PP, Pun BT, Herr DL, Maze M, Girard TD, Miller RR, Shintani AK, Thompson JL, Jackson JC, Deppen SA, Stiles RA, Dittus RS, Bernard GR, Ely EW: Effect of sedation with dexmedetomidine vs lorazepam on acute brain dysfunction in mechanically ventilated patients: the MENDS randomized controlled trial. JAMA 2007, 298: 2644-2653. 10.1001/jama.298.22.2644
Martin J, Parsch A, Franck M, Wernecke KD, Fischer M, Spies C: Practice of sedation and analgesia in German intensive care units: results of a national survey. Crit Care 2005, 9: R117-R123. 10.1186/cc3035
Kim MH, Hahn TH: The effect of clonidine pretreatment on the perioperative proinflammatory cytokines, cortisol, and ACTH responses in patients undergoing total abdominal hysterectomy. Anesth Analg 2000, 90: 1441-1444. 10.1097/00000539-200006000-00035
Taniguchi T, Kurita A, Kobayashi K, Yamamoto K, Inaba H: Dose- and time-related effects of dexmedetomidine on mortality and inflammatory responses to endotoxin-induced shock in rats. J Anesth 2008, 22: 221-228. 10.1007/s00540-008-0611-9
Hofer S, Steppan J, Wagner T, Funke B, Lichtenstern C, Martin E, Graf BM, Bierhaus A, Weigand MA: Central sympatholytics prolong survival in experimental sepsis. Crit Care 2009, 13: R11. 10.1186/cc7709
Rosas-Ballina M, Ochani M, Parrish WR, Ochani K, Harris YT, Huston JM, Chavan S, Tracey KJ: Splenic nerve is required for cholinergic antiinflammatory pathway control of TNF in endotoxemia. Proc Natl Acad Sci USA 2008, 105: 11008-11013. 10.1073/pnas.0803237105
Ulloa L, Deitch EA: Neuroimmune perspectives in sepsis. Crit Care 2009, 13: 133. 10.1186/cc7758
Correa-Sales C, Tosta CE, Rizzo LV: The effects of anesthesia with thiopental on T lymphocyte responses to antigen and mitogens in vivo and in vitro. Int J Immunopharmacol 1997, 19: 117-128. 10.1016/S0192-0561(97)00003-9
Yang FL, Li CH, Hsu BG, Tsai NM, Lin SZ, Harn HJ, Chen HI, Liao KW, Lee RP: The reduction of tumor necrosis factor-alpha release and tissue damage by pentobarbital in the experimental endotoxemia model. Shock 2007, 28: 309-316.
Ploppa A, Kiefer RT, Nohe B, Haeberle HA, Dieterich HJ, Unertl KE, Krueger WA: Dose-dependent influence of barbiturates but not of propofol on human leukocyte phagocytosis of viable Staphylococcus aureus. Crit Care Med 2006, 34: 478-483. 10.1097/01.CCM.0000199067.71968.6E
Kress JP, Pohlman AS, O'Connor MF, Hall JB: Daily interruption of sedative infusions in critically ill patients undergoing mechanical ventilation. N Engl J Med 2000, 342: 1471-1477. 10.1056/NEJM200005183422002
Girard TD, Kress JP, Fuchs BD, Thomason JW, Schweickert WD, Pun BT, Taichman DB, Dunn JG, Pohlman AS, Kinniry PA, Jackson JC, Canonico AE, Light RW, Shintani AK, Thompson JL, Gordon SM, Hall JB, Dittus RS, Bernard GR, Ely EW: Efficacy and safety of a paired sedation and ventilator weaning protocol for mechanically ventilated patients in intensive care (Awakening and Breathing Controlled trial): a randomised controlled trial. Lancet 2008, 371: 126-134. 10.1016/S0140-6736(08)60105-1
Schweickert WD, Pohlman MC, Pohlman AS, Nigos C, Pawlik AJ, Esbrook CL, Spears L, Miller M, Franczyk M, Deprizio D, Schmidt GA, Bowman A, Barr R, McCallister KE, Hall JB, Kress JP: Early physical and occupational therapy in mechanically ventilated, critically ill patients: a randomised controlled trial. Lancet 2009, 373: 1874-1882. 10.1016/S0140-6736(09)60658-9
Papadimos TJ, Hensley SJ, Duggan JM, Khuder SA, Borst MJ, Fath JJ, Oakes LR, Buchman D: Implementation of the "FASTHUG" concept decreases the incidence of ventilator-associated pneumonia in a surgical intensive care unit. Patient Saf Surg 2008, 2: 3. 10.1186/1754-9493-2-3
Blamoun J, Alfakir M, Rella ME, Wojcik JM, Solis RA, Anees KM, DeBari VA: Efficacy of an expanded ventilator bundle for the reduction of ventilator-associated pneumonia in the medical intensive care unit. Am J Infect Control 2009, 37: 172-175. 10.1016/j.ajic.2008.05.010
Resar R, Pronovost P, Haraden C, Simmonds T, Rainey T, Nolan T: Using a bundle approach to improve ventilator care processes and reduce ventilator-associated pneumonia. Jt Comm J Qual Patient Saf 2005, 31: 243-248.
Berriel-Cass D, Adkins FW, Jones P, Fakih MG: Eliminating nosocomial infections at Ascension Health. Jt Comm J Qual Patient Saf 2006, 32: 612-620.
Youngquist P, Carroll M, Farber M, Macy D, Madrid P, Ronning J, Susag A: Implementing a ventilator bundle in a community hospital. Jt Comm J Qual Patient Saf 2007, 33: 219-225.
Unahalekhaka A, Jamulitrat S, Chongsuvivatwong V, Ovretveit J: Using a collaborative to reduce ventilator-associated pneumonia in Thailand. Jt Comm J Qual Patient Saf 2007, 33: 387-394.
Wip C, Napolitano L: Bundles to prevent ventilator-associated pneumonia: how valuable are they? Curr Opin Infect Dis 2009, 22: 159-166. 10.1097/QCO.0b013e3283295e7b
Brook AD, Ahrens TS, Schaiff R, Prentice D, Sherman G, Shannon W, Kollef MH: Effect of a nursing-implemented sedation protocol on the duration of mechanical ventilation. Crit Care Med 1999, 27: 2609-2615. 10.1097/00003246-199912000-00001
Arias-Rivera S, Sanchez-Sanchez MM, Santos-Diaz R, Gallardo-Murillo J, Sanchez-Izquierdo R, Frutos-Vivar F, Ferguson ND, Esteban A: Effect of a nursing-implemented sedation protocol on weaning outcome. Crit Care Med 2008, 36: 2054-2060. 10.1097/CCM.0b013e31817bfd60
de Wit M, Gennings C, Jenvey WI, Epstein SK: Randomized trial comparing daily interruption of sedation and nursing-implemented sedation algorithm in medical intensive care unit patients. Crit Care 2008, 12: R70. 10.1186/cc6908
Elliott R, McKinley S, Aitken LM, Hendrikz J: The effect of an algorithm-based sedation guideline on the duration of mechanical ventilation in an Australian intensive care unit. Intensive Care Med 2006, 32: 1506-1514. 10.1007/s00134-006-0309-0
Bucknall TK, Manias E, Presneill JJ: A randomized trial of protocol-directed sedation management for mechanical ventilation in an Australian intensive care unit. Crit Care Med 2008, 36: 1444-1450. 10.1097/CCM.0b013e318168f82d
Quenot JP, Ladoire S, Devoucoux F, Doise JM, Cailliod R, Cunin N, Aube H, Blettery B, Charles PE: Effect of a nurse-implemented sedation protocol on the incidence of ventilator-associated pneumonia. Crit Care Med 2007, 35: 2031-2036. 10.1097/01.ccm.0000282733.83089.4d
von Dossow V, Luetz A, Haas A, Sawitzki B, Wernecke KD, Volk HD, Spies CD: Effects of remifentanil and fentanyl on the cell-mediated immune response in patients undergoing elective coronary artery bypass graft surgery. J Int Med Res 2008, 36: 1235-1247.
Arya V, Ramji S: Midazolam sedation in mechanically ventilated newborns: a double blind randomized placebo controlled trial. Indian Pediatr 2001, 38: 967-972.
Helmy SA, Al Attiyah RJ: The immunomodulatory effects of prolonged intravenous infusion of propofol versus midazolam in critically ill surgical patients. Anaesthesia 2001, 56: 4-8. 10.1046/j.1365-2044.2001.01713.x
Kress JP, O'Connor MF, Pohlman AS, Olson D, Lavoie A, Toledano A, Hall JB: Sedation of critically ill patients during mechanical ventilation. A comparison of propofol and midazolam. Am J Respir Crit Care Med 1996, 153: 1012-1018.
Liatsi D, Tsapas B, Pampori S, Tsagourias M, Pneumatikos I, Matamis D: Respiratory, metabolic and hemodynamic effects of clonidine in ventilated patients presenting with withdrawal syndrome. Intensive Care Med 2009, 35: 275-281. 10.1007/s00134-008-1251-0
Riker RR, Shehabi Y, Bokesch PM, Ceraso D, Wisemandle W, Koura F, Whitten P, Margolis BD, Byrne DW, Ely EW, Rocha MG: Dexmedetomidine vs midazolam for sedation of critically ill patients: a randomized trial. JAMA 2009, 301: 489-499. 10.1001/jama.2009.56
Nadal P, Nicolas JM, Font C, Vilella A, Nogue S: Pneumonia in ventilated head trauma patients: the role of thiopental therapy. Eur J Emerg Med 1995, 2: 14-16. 10.1097/00063110-199503000-00004
Becker G, Blum HE: Novel opioid antagonists for opioid-induced bowel dysfunction and postoperative ileus. Lancet 2009, 373: 1198-1206. 10.1016/S0140-6736(09)60139-2
Thomas J, Karver S, Cooney GA, Chamberlain BH, Watt CK, Slatkin NE, Stambler N, Kremer AB, Israel RJ: Methylnaltrexone for opioid-induced constipation in advanced illness. N Engl J Med 2008, 358: 2332-2343. 10.1056/NEJMoa0707377
The authors have no potential conflicts of interest to declare and no involvement in any organization with a direct financial interest in the subject of the manuscript.
The authors declare that they have no competing interests.
SN, DeM, DaM, AD, and CHM designed this review. SN, and DeM collected data. SN wrote the manuscript, and all authors participated in its critical revision. SN had full access to all data in the study and had final responsibility for the decision to submit for publication. All authors read and approved the final manuscript.
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Nseir, S., Makris, D., Mathieu, D. et al. Intensive care unit-acquired infection as a side effect of sedation. Crit Care 14, R30 (2010). https://doi.org/10.1186/cc8907
- Mechanical Ventilation
- Intensive Care Unit Patient
- Intensive Care Unit Stay