In vivo study of endothelial barrier-related GTPase expression in the kidney and liver during the acute phase of nonlethal sepsis
© Zychar et al.; licensee BioMed Central Ltd. 2014
Published: 3 December 2014
Maintenance of the integrity of the endothelial barrier is crucial in pathological inflammatory/infectious conditions . The endothelial barrier dysfunction leading to increased vascular permeability and leukocyte transmigration in the systemic inflammatory state has been intrinsically related to the multiple organ dysfunction syndrome. The Rho GTPases family regulates the organization of the actin cytoskeleton, and plays a fundamental role in maintaining homeostasis and function of the endothelial barrier . The study of the mechanisms of intracellular signaling related to the integrity of the endothelial barrier may help in understanding the hemodynamic changes during systemic infection. Thus, this study aimed to correlate the pattern of genic expression of GTPases during the initial periods of sepsis in order to understand the kinetic of these molecular mechanisms in organs often affected in sepsis.
Wistar rats weighting 200 to 250 g were submitted to: nonlethal sepsis (2 ml Escherichia coli 107 UFC/ml i.v. inoculation [3, 4], n = 5, S7 group); minor trauma (cervical incision with catheter implantation in jugular, injection of 2 ml saline, n = 5, SHAM group); without any procedure (n = 5, NAIVE group). After 2 and 6 hours, the liver and kidney were collected to determine RhoA, Rac1 and Cdc42 gene expression by quantitative real-time PCR.
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