Figure 2From: In vivo study of endothelial barrier-related GTPase expression in the kidney and liver during the acute phase of nonlethal sepsisGene expression of GTPases RhoA (A), Rac1 (B) and Cdc42 (C) in the kidney of rats. Animals were subjected to nonlethal sepsis 2 ml Escherichia coli 107 UFC/ml i.v. inoculation (S7 group), minor trauma 2 ml saline i.v. inoculation (SHAM group), or animals without inoculation were used as control group (NAÏVE group). After 2 or 6 hours the kidney was isolated. Total RNA was extracted and transcribed to cDNA. Gene expression was quantified by real-time PCR. Graph bars show the relative concentration of each cDNA compared with the NAIVE group and the housekeeping gene HPRT. Results are mean ± SEM (n = 5 animals/group) and analyzed in triplicate. *P < 0.05 compared to NAÏVE group.Back to article page