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Figure 1 | Critical Care

Figure 1

From: In vivo study of endothelial barrier-related GTPase expression in the kidney and liver during the acute phase of nonlethal sepsis

Figure 1

Gene expression of GTPases RhoA (A), Rac1 (B) and Cdc42 (C) in the liver of rats. Animals were subjected to nonlethal sepsis 2 ml Escherichia coli 107 UFC/ml i.v. inoculation (S7 group), minor trauma 2 ml saline i.v. inoculation (SHAM group), or animals without inoculation were used as control group (NAÏVE group). After 2 or 6 hours the kidney was isolated. Total RNA was extracted and transcribed to cDNA. Gene expression was quantified by real-time PCR. Graph bars show the relative concentration of each cDNA compared with the NAIVE group and the housekeeping gene HPRT. Results are mean ± SEM (n = 5 animals/group) and analyzed in triplicate. *P < 0.05 compared to NAÏVE group.

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Critical Care

ISSN: 1364-8535