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Telomere shortening during human septic shock: influence of sepsis mediators, role in organ failures, and septic myocardial dysfunction
Critical Care volume 25, Article number: 401 (2021)
Leucocyte telomere length (LTL) is widely considered a marker of cellular ageing. Telomere attrition has been involved in cardiovascular disorders as a result of inflammatory stress , but has been scarcely evaluated in acute settings. Septic shock is associated with an overwhelming inflammatory reaction that may be involved in the genesis of organ failure, including septic myocardial dysfunction . The present study aimed to assess whether septic shock is associated with telomere attrition and evaluate the role of sepsis mediators and the impact on organ failures. Fifty-five patients free of chronic heart failure who met septic shock criteria (as defined according to the ACCP/SCCM Consensus Conference) were prospectively included at the medical intensive care unit of Henri-Mondor University Hospital (Créteil, France).
LTL was measured by QuantStudio™ 6 Flex Real-Time quantitative PCR System (Applied Biosystems, Foster, CA); see Table 1 legend. LTL was assessed in septic shock patients (in 55 and 24 patients on day-1 and day-2, respectively) and in 55 healthy controls matched to septic shock patients for age (± 3 years) and gender.
Septic myocardial dysfunction (SMD) was defined as a depressed left ventricle ejection fraction (< 45% or when an inotrope infusion was needed to achieve a value ≥ 45%) on echocardiography performed on day-1 or day-2 of septic shock .
We assessed plasma concentration of 24 putative sepsis mediators on day-1, including inflammatory markers (IL-1α, IL-1β, IL1-RA, IL-6, IL-10, IL-12, IL-15, IL-17, IL-33, IFN-γ, TNF-α, CD40L, HSP70, sFAS, sFAS ligand, sST2, granzyme, TRAIL, PAI1, and VEGF), chemokines (IL-8, MCP1), and adhesion molecules (sVCAM, sICAM). sST2 and the remaining sepsis mediators were measured with human magnetic Luminex screening assay (R&D, Bio-Techne, Lille, France), and a multi-analyte Milliplex human cytokine kit (Millipore Corporation, Billerica, MA, USA), respectively, and were analyzed using fluorescence intensities .
Organ failures and patient severity were assessed using the Sequential Organ Failure Assessment (SOFA) and Simplified Acute Physiology Score II (SAPS II) score, respectively. Spearman bivariate correlations were used to build a focused principal component analysis (FPCA; “psy” package in R), using LTL as the dependent variable and allowing a simple graphical display of correlation structures.
LTL was similar between controls and septic shock patients (Fig. 1a). We observed a correlation between LTL and age in the control group as expected (Spearman’s rho = − 0.29, p = 0.04) but not in the septic shock group (rho = − 0.03, p = 0.82). Table 1 shows the clinical characteristics of septic shock patients according to LTL (below or above median value) on day-1; all variables were similar between groups, except for more female gender in patients with higher LTL. There was no statistically significant correlation between LTL on the one hand and SMD (Fig. 1b), SOFA score, SAPS II score, or sepsis mediators (except for sST2, sFASL and granzyme) on the other hand (Fig. 1c). LTL was similar in septic shock survivors and nonsurvivors at day-1, but decreased between day-1 and day-2 in survivors (Fig. 1d).
LTL during septic shock may be determined by factors other than the inflammatory mediators we herein assessed; a prominent role for oxidative stress needs to be assessed in future studies. The association of LTL with FasL is in accordance with a previous study showing a decreased production of FasL after TCR/CD3 signaling of senescent T cells . Senescence was also associated with reduced expression of the effector molecules granzyme and perforin . The decrease in LTL in septic shock survivors may be explained by a relative hyperfunction of leucocyte against infection in this subgroup . We did not find a correlation between LTL and organ failures, as previously reported by Liu et al. .
Availability of data and materials
The datasets supporting the conclusions are included within the article.
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No funding was received for this study.
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The study was approved by institutional review board (CPP Ile de France IX).
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Razazi, K., Marcos, E., Hüe, S. et al. Telomere shortening during human septic shock: influence of sepsis mediators, role in organ failures, and septic myocardial dysfunction. Crit Care 25, 401 (2021). https://doi.org/10.1186/s13054-021-03818-9