- Open Access
Prediction of hospital outcome in septic shock: a prospective comparison of tissue Doppler and cardiac biomarkers
Critical Care volume 14, Article number: R44 (2010)
Diastolic dysfunction as demonstrated by tissue Doppler imaging (TDI), particularly E/e' (peak early diastolic transmitral/peak early diastolic mitral annular velocity) is common in critical illness. In septic shock, the prognostic value of TDI is undefined. This study sought to evaluate and compare the prognostic significance of TDI and cardiac biomarkers (B-type natriuretic peptide (BNP); N-terminal proBNP (NTproBNP); troponin T (TnT)) in septic shock. The contribution of fluid management and diastolic dysfunction to elevation of BNP was also evaluated.
Twenty-one consecutive adult patients from a multidisciplinary intensive care unit underwent transthoracic echocardiography and blood collection within 72 hours of developing septic shock.
Mean ± SD APACHE III score was 80.1 ± 23.8. Hospital mortality was 29%. E/e' was significantly higher in hospital non-survivors (15.32 ± 2.74, survivors 9.05 ± 2.75; P = 0.0002). Area under ROC curves were E/e' 0.94, TnT 0.86, BNP 0.78 and NTproBNP 0.67. An E/e' threshold of 14.5 offered 100% sensitivity and 83% specificity. Adjustment for APACHE III, cardiac disease, fluid balance and grade of diastolic function, demonstrated E/e' as an independent predictor of hospital mortality (P = 0.019). Multiple linear regression incorporating APACHE III, gender, cardiac disease, fluid balance, noradrenaline dose, C reactive protein, ejection fraction and diastolic dysfunction yielded APACHE III (P = 0.033), fluid balance (P = 0.001) and diastolic dysfunction (P = 0.009) as independent predictors of BNP concentration.
E/e' is an independent predictor of hospital survival in septic shock. It offers better discrimination between survivors and non-survivors than cardiac biomarkers. Fluid balance and diastolic dysfunction were independent predictors of BNP concentration in septic shock.
Septic shock in adults refers to a state of acute circulatory failure characterized by persistent arterial hypotension unexplained by other causes . Although this clinical syndrome is heterogeneous with regard to factors such as causal micro-organism, patient predisposition, co-morbidity and response to therapy, a key element and unifying feature is the manifestation of cardiovascular dysfunction. Although the underlying cause of death in septic shock is often multifactorial, refractory hypotension and cardiovascular collapse are frequently observed in the terminal phases of the condition . Whilst impaired systolic function has been identified as the major culprit, the contribution of diastolic dysfunction (and hence ventricular filling) to cardiovascular morbidity and mortality in septic shock is not fully understood. Investigation of left ventricular (LV) diastolic function at the bedside is challenging, but techniques such as echocardiography and biomarkers such as B-type natriuretic peptide (BNP) are increasingly supported by current literature [3–5]. In particular, recent application of non-invasive, bedside technologies, such as tissue Doppler imaging (TDI), offer fresh insight .
TDI is an echocardiographic technique that measures myocardial velocities , which are low frequency, high-amplitude signals filtered from conventional Doppler imaging . TDI has gained acceptance amongst cardiologists for the evaluation of diastolic function, particularly as a measure of ventricular relaxation and ventricular filling pressure . However, there are scant data regarding its use in critical care. TDI has demonstrated prognostic utility in a range of cardiovascular diseases , including following myocardial infarction [11, 12], heart failure [13–16], abnormal LV function at dobutamine echocardiography , nonvalvular atrial fibrillation , hypertension , and end-stage renal disease .
Previously, we demonstrated that evidence of diastolic dysfunction on TDI is common in critically ill patients . The significance of this was recently highlighted by Ikonomidis and colleagues, who demonstrated that TDI may be prognostically useful in the general ICU population . To date, the prognostic significance of this technique has not been specifically evaluated in septic shock.
Cardiac biomarkers including BNP [23, 24], N-terminal proBNP (NTproBNP)  and troponin  potentially offer prognostic information in the critically ill. To date, no comparison has been made between TDI and cardiac biomarkers (BNP, NTproBNP and troponin) with regard to prediction of hospital outcome in septic shock.
This study sought to evaluate and compare the prognostic significance of TDI variables and cardiac biomarkers in septic shock. An auxiliary aim was to evaluate the potential contribution of LV diastolic dysfunction and fluid management to elevation of plasma BNP concentrations in septic shock.
Materials and methods
This prospective observational study was approved by the Princess Alexandra Hospital Human Research Ethics Committee (project 2005/213), and the Guardianship and Administration Tribunal of Queensland (project 2006/07) and informed consent was obtained from the patient or legally authorized representative where appropriate.
Twenty-one consecutive adult patients with septic shock were recruited from the ICU during an 11-month period (May 2005 to March 2006). Eligible patients were enrolled within 72 hours of admission to the ICU with septic shock or development of septic shock while in the ICU. Septic shock was defined as severe sepsis with persistent hypotension (ie. with a mean arterial pressure (MAP) < 60 mmHg or a reduction in systolic blood pressure (SBP) > 40 mmHg from baseline) despite adequate volume resuscitation in the absence of other causes for hypotension .
Exclusion criteria included: age younger than 18 years; presence of moderate to severe valvular heart disease; or patient or legally authorized representative declined participation.
Patient care followed standard practice. Clinical fluid resuscitation and management were undertaken in a fashion consistent with surviving sepsis guidelines . More specifically, fluid challenges were undertaken incrementally while clinical response was observed. Therapeutic variables considered in determining the requirement and response to fluid management included pulse rate, blood pressure (target MAP > 65 mmHg), peripheral perfusion, urine output (target > 0.5 ml/kg/hr), and central venous pressure (CVP). Research measurements were not released to the treating clinician.
Clinical and outcome data
Clinical data included height, weight, ventilation mode and settings, heart rate, rhythm, arterial blood pressure (SBP; diastolic blood pressure (DBP); MAP) and CVP. Body surface area (BSA) was calculated . ICU fluid balance was recorded for the study day (fluid balance). Vasopressor/inotropic infusion rates and, ICU and hospital length of stay and outcome were recorded. Illness severity was quantified using (Acute Physiology and Chronic Health Evaluation) APACHE III and Sequential Organ Failure Assessment (SOFA) scores. Patients were considered to have a history of cardiac disease if they had prior or current ischemic heart disease (angina or myocardial infarction) or cardiac surgery.
Transthoracic echocardiography and Doppler examinations were performed by experienced echocardiographers (coordinated by Jenkins C) using commercially available echocardiographic equipment (Acuson Sequoia, Siemens AG, Munich, Germany and Sonos 7500, Philips Medical Systems, Andover, MA, USA). Measurements were made off-line, using AccessPoint™ 2000 software (Freeland Systems, Westfield, IN, USA). Unless otherwise stated, measurements were made in triplicate at end expiration.
LV end-diastolic volume (LVEDV) and LV end-systolic volume (LVESV) were calculated using the biplane method of disks (modified Simpson's rule) from the apical four-chamber and two-chamber views  and indexed to BSA (LVEDVI and LVESVI, respectively). LV ejection fraction (LVEF) was calculated as (LVEDV - LVESV)/LVEDV × 100. Systolic dysfunction was defined as EF below 55%. LV outflow tract diameter (OTD) was recorded as the maximum measurement from triplicate zoomed parasternal long axis view.
Transmitral flow velocities were recorded with pulsed-wave Doppler with the sample volume placed at the mitral valve tips from the apical four-chamber view . Peak passive (E) and active (A) velocities were recorded. E wave deceleration time (DT) was measured. E to A ratio (E/A) was calculated.
Doppler interrogation of LV outflow tract velocity was guided by apical five-chamber view . Heart rate (HR), velocity time integral (VTI) and peak velocity (Vpeak) were measured. Stroke volume was calculated as the product of VTI and cross-sectional area of the LV outflow tract [π.(OTD/2)2]. Cardiac output was calculated as the product of stroke volume and HR. Stroke volume and cardiac output measurements were indexed to body surface area (SVI and CI, respectively).
Myocardial velocities were obtained using tissue Doppler settings, with the pulsed-wave Doppler sample volume at the septal mitral annulus in the apical four-chamber view. Peak systolic (s'), early diastolic (e') and late diastolic (a') myocardial velocities were measured. E/e' was calculated. When A and/or a' were indistinguishable due to sinus tachycardia, E and/or e' were measured as described by Nagueh and colleagues . In the presence of atrial dysrhythmia, transmitral and tissue Doppler velocities were measured over five consecutive cardiac cycles .
As previously described , thresholds for abnormal diastolic TDI were accepted as e'less than 9.6 cm/s (myocardial relaxation below the lower 95% confidence limit of normal subjects)  or E/e' more than 15 (mean LV end-diastolic pressure > 15 mmHg) .
Guidelines previously published by our group were used to grade LV diastolic function as normal, impaired relaxation, pseudonormal or restrictive . Age-dependent thresholds for deceleration time (< 40 years < 220 ms; 40 to 60 years 140 to 250 ms; > 60 years 140 to 275 ms) were used to determine impaired relaxation (DT above normal limit) and restrictive patterns (DT below normal limit). In order to distinguish between normal and pseudonormal patterns, we incorporated E/e' (normal < 8; pseudonormal > 15). Where E/e' was inconclusive (8 to 15), increased left atrial area (> 20 cm) was used as a marker of raised LV filling pressure (pseudonormal pattern). Patients categorized other than normal were considered to have diastolic dysfunction.
Plasma BNP concentration was measured using a Biosite Triage® immunoassay (Biosite Diagnostics, San Diego, CA, USA), Plasma Troponin T (TnT; Elecsys® Troponin T, 3rd generation immunoassay; Roche Diagnostics Australia Pty Ltd, Castle Hill, NSW, Australia) and NTProBNP concentration (Elecsys® proBNP, Roche Diagnostics Australia Pty Ltd, Castle Hill, NSW, Australia) were run on Roche Elecsys® analyzers (Roche Diagnostics Australia Pty Ltd, Castle Hill, NSW, Australia). Plasma C reactive protein (CRP) concentration was measured using an immunotubidometric assay (UniCel® DxI 800 Access® Immunoassay System, Beckman Coulter Australia Pty. Ltd., Gladesville, NSW, Australia). Laboratory thresholds were used to determine elevation of biomarkers: BNP (normal < 100 ng/L), NTproBNP (0 to 50 years < 450; 50 to 75 years < 900; > 75 years < 1800 ng/L), TnT (< 0.03 μg/L) and CRP (< 5.0 mg/L).
Coded echocardiographic and Doppler recordings were analyzed at least one month after acquisition by a single observer blinded to clinical and biochemical data. Biochemical assay was performed on coded samples by technicians blinded to clinical and echocardiographic data.
Analysis was performed by SPSS, version 14.0 for Windows (SPSS Inc., Chicago, IL, USA). Descriptive measures were used to evaluate the distribution of variables. Differences between groups were assessed using Fisher's exact test for categorical data. Continuous data were assessed using Levene's test for equality of variance before applying Student's t-test for independent samples. BNP and NTproBNP concentrations were log-transformed to achieve normality before application of linear regression techniques. Discrimination between hospital survivors and non-survivors was evaluated by receiver operating characteristic (ROC) curve analysis.
Cox proportional hazards regression was used for time to event outcomes (hospital survival) from the time of echocardiography. Adjustment was made for the potential influence of cardiac disease, fluid balance and grade of diastolic dysfunction upon E/e'.
Multiple-linear regression analyses were undertaken to determine contributions to BNP concentration (lnBNP). Potential predictor variables included APACHE III score (first ICU day), gender, , cardiac disease , intravenous fluid therapy , noradrenaline dose , CRP , LVEF , and LV diastolic dysfunction . A backwards elimination procedure was then used to discard predictor variables with P < 0.1 in multiple regression models one by one until a final 'best' model was achieved.
In final analyses, a P-value less than 0.05 was regarded as significant. Unless stated otherwise, results are reported as mean ± standard deviation (SD) (range).
Subgroup analysis of septic patients from data previously published by our group yielded a mean ± SD E/e' of 11.4 ± 5 (range: 3.59 to 23.15) and hospital mortality of 30% . It was determined that a sample of 20 patients would allow detection of a mean difference in E/e' of 4 or more between survivors and non-survivors (80% power; α = 0.05) .
Twenty-one consecutive septic shock patients were studied (Table 1). Fifteen participants (71%) were studied within 24 hours of developing septic shock. Variables recorded on the study day are presented in Table 2.
Sixteen patients (76%) were mechanically ventilated at the time of the initial assessment. The requirement for mechanical ventilation did not distinguish survivors from non-survivors (P = 0.15). Of the mechanically ventilated patients, positive end-expiratory pressure (PEEP) requirements were not different between survivors and non-survivors (7.05 ± 3 cmH2O vs 7.9 ± 5.1 cmH2O, respectively; P = 0.67).
Eleven patients (52%) were in normal sinus rhythm and two (9.5%) were paced. Noradrenaline infusion was running at the time of initial assessment in seventeen patients (81%; Mean ± SD infusion rate 0.124 ± 0.12 micrograms/kg/min). In addition to noradrenaline, one patient was receiving adrenaline and one was receiving dopamine. Mean ± SD fluid balance on the day of study was 1780 ± 1848 mL (range: -1734 to 5320).
The diagnosis of cardiac disease (Table 1) was based on previous history (non-acute) in seven out of nine patients. Of the remaining patients, one developed sepsis secondary to wound infection eight days following aortic root and valve replacement (no significant coronary artery disease; survived to hospital discharge), whereas the other developed pneumonia sixteen days following emergency coronary artery bypass grafting for acute myocardial infarction (non-survivor). No patients had a previous history of heart failure. Fourteen patients had been receiving treatment for hypertension prior to the development of septic shock. Six patients had been previously diagnosed with diabetes mellitus (n = 5; all type 2) or glucose intolerance prior to the development of septic shock.
Systolic dysfunction (EF < 55%) was evident in 14 patients (67%). Transthoracic measurement of e' and E/e' was feasible in 20 of 21 patients. Fusion of E and A waves was observed in four examinations (19%). Fusion of e'and a' waves was observed in three (15%). At initial assessment, e' was less than 9.6 cm/s in 11 (55%) patients. At this time, E/e' was more than 15 in three (15%), 8 to 15 in thirteen (65%) and less than 8 in four (20%) patients. TDI variables (including e', a', s' and E/e') were not significantly different between ventilated and non-ventilated patients. Diastolic function was graded as normal in nine (43%), impaired relaxation in three (14%), pseudonormal in seven (33%) and restrictive in two patients (10%). Thus, diastolic dysfunction was present in 57% of patients (n = 12).
BNP was elevated in fifteen patients (71%), NTproBNP in six (28%) and TnT in fourteen (67%).
Significant differences were observed between hospital survivors and non-survivors (Table 3) with respect to E/e' (survivor 9.05 ± 2.75, non-survivor 15.32 ± 2.74; P = 0.0002), e' (survivor 10.4 ± 3.4 cm/s, non-survivor 6.8 ± 1.9 cm/s; P = 0.025) and s' (survivor 13 ± 3.7 cm/s, non-survivor 8.6 ± 4.1 cm/s; P = 0.03). The area under the ROC curve (c statistic) for each of these variables was 0.94 for E/e', 0.86 for e'and 0.83 for s'. An E/e' threshold value of 14.5 offered sensitivity of 100% and specificity of 83% (Figure 1). The c statistic of 0.86 for TnT, 0.78 for BNP and 0.67 for NTproBNP. No difference in LVEF (systolic function) was observed (survivor 43 ± 15%, non-survivor 43 ± 14%; P = 0.91).
Prediction of hospital survival
Univariate Cox regression analysis (Table 3) yielded significant associations between survival to hospital discharge and E/e' (P = 0.005), e' (P = 0.04), s' (P = 0.048) and TnT (P = 0.03). Adjustment for APACHE III score, history of cardiac disease, fluid balance and grade of diastolic function, revealed E/e' as an independent predictor of hospital mortality (P = 0.019). A Kaplan-Meier plot of the association between E/e' and survival to hospital discharge is shown in Figure 2.
Plasma BNP concentration
From an initial model containing APACHE III score, gender, cardiac disease, fluid balance, noradrenaline dose, CRP, EF and diastolic dysfunction, the backward elimination procedure yielded a 'best' model containing gender (P = 0.089), APACHE III score (P = 0.033), fluid balance (P = 0.001) and diastolic dysfunction (P = 0.009). This final model accounted for 71.3% of variation in lnBNP concentration (adjusted R square 0.713).
The cardinal finding of this study is that E/e' offers independent and better prognostic prediction of hospital outcome in septic shock as compared with cardiac biomarkers (BNP, NTproBNP, TnT). We also observed that conventional measures of systolic function, such as EF and SVI (Table 3) did not discriminate between hospital survivors and non-survivors. This study also demonstrates that fluid balance and diastolic dysfunction are independent predictors of BNP concentration in septic shock patients.
Diastolic function and tissue Doppler imaging in septic shock
We have demonstrated an association between TDI indices of diastolic function and outcome in septic shock. The significance of this important new finding is highlighted by the superiority of these variables over the measures of cardiac systolic function and cardiac biomarkers incorporated into this study. Despite demonstrating value in a range of cardiovascular diseases , and more recently in a study of general ICU patients by Ikonomidis and colleagues , the prognostic potential of TDI in septic shock per se has not previously been reported.
Our demonstration of an association between diastolic function and mortality in septic shock complements previous data. In a radionuclide cineangiographic study, Parker and colleagues documented that non-survivors did not demonstrate LV dilation ('preload adaptation') and therefore were unable to maintain stroke volume and cardiac output [43, 44]. Also, Munt and colleagues demonstrated DT as an independent predictor of mortality in severe sepsis . In addition to our TDI findings, we observed a trend toward an association between DT and hospital mortality (P = 0.07). Although no clear functional relation has been demonstrated, sepsis-induced diastolic dysfunction is likely to be associated with a range of histologic abnormalities such as inflammatory infiltrate, interstitial edema, apoptosis, and necrosis [46, 47].
The peak early diastolic mitral annular velocity (E)', as measured by TDI, reflects LV relaxation [48, 49]. Although this variable appears not to be as preload insensitive as originally proposed [49, 50], it is increasingly valued as a quantitative index of LV diastolic function. This is because it does not pseudo-normalize in the same way as transmitral flow . Also, the E/e' ratio has been proposed as an estimate of LV filling pressure that corrects E velocity for the influence of myocardial relaxation [33, 52].
Two recent studies have utilized TDI in the evaluation of septic ICU patients. McLean and colleagues used E/e' as an estimate of LV filling pressure in their prognostic study of BNP in patients with severe sepsis and septic shock . They reported E/e' to be non-significantly lower in non-survivors (survivors 14.8 ± 7.4, non-survivors 12.1 ± 4.6; P = 0.452). However, their study incorporated a number of patients with severe sepsis (lower severity of illness compared with the current study), and did not report fluid management, which is an important determinant of survival in sepsis . Bouhemad and colleagues  used TDI to demonstrate isolated and reversible impairment of ventricular relaxation in septic shock patients with increased plasma troponin I concentration but associations with mortality were not assessed.
Cardiac biomarkers including BNP [23, 24], NTproBNP  and troponin  have been offered as potential prognostic tools in the critically ill. Our study demonstrates the superiority of TDI over these biomarkers. This is potentially explained by the magnitude of potential confounders on plasma biomarker concentrations in the critically ill . Furthermore, TDI offers more direct evaluation of myocardial function.
B-type natriuretic peptide
In general, BNP is a peptide hormone secreted by the ventricular myocardial in response to wall stress . Its principal clinical use is the diagnosis of heart failure . However, elevated BNP appears to lack validity as a biomarker of myocardial dysfunction in sepsis. Potential explanations include inflammation , altered clearance , altered intrathoracic pressures/mechanical ventilation , vasoactive and inotropic drugs , fluid management [36, 59], and diastolic dysfunction .
On the basis of previous laboratory data  and our own clinical research , we incorporated an auxiliary aim of the current study of evaluating the potential influence of fluid management on plasma BNP concentrations in septic shock. Also, the relation between diastolic function and plasma BNP concentration had not been evaluated in septic shock. We are the first to demonstrate fluid balance and diastolic dysfunction as independent predictors of plasma BNP concentration in septic shock.
In keeping with international guidelines for hemodynamic monitoring in shock, our unit does not routinely use pulmonary artery catheters , and LV filling pressure is not pursued as a therapeutic target. Although incorporation of pulmonary artery catheter data might have yielded interesting comparisons, it was unnecessary to achieve or stated aims and might have impaired the feasibility of our study. We propose that the resultant observational data forms a robust reflection of clinical practice in the context of contemporary sepsis management. Based on our findings, additional research incorporating pulmonary artery catheterization might now be justified.
We have reported TDI measurements taken at the septal mitral annulus. This technique was based on results reported by Ommen and colleagues demonstrating good prediction of LV end-diastolic pressure . Although the feasibility of this approach in critical care is appealing, the mean of measurements sampled around the perimeter of the mitral valve would be less susceptible to regional wall motion abnormalities, if present . The potential influence of mechanical ventilation, right ventricular function and inotropes/vasopressors upon tissue Doppler variables is unclear. Our observational study has not been designed to clarify these potential interactions but based on the current findings, further research in these areas is justified.
In clinical studies, it is challenging to standardize data collection at a fixed time from onset of sepsis. We studied patients within 72 hours of development of septic shock (admission to ICU or onset in ICU). The strength of this design is that it potentially optimizes the comparison of TDI with cardiac biomarkers, particularly BNP , as predictors of outcome. Due to an inability to predict the development of sepsis, we were unable to define the pre-morbid diastolic function of the study participants.
Potential clinical significance and directions for future research
The findings of this study are of potential clinical importance. First, TDI might prove useful in risk stratification. This may help identify septic shock patients requiring more intensive therapy based upon their diastolic performance. Secondly, the association between diastolic dysfunction and mortality might offer a novel therapeutic target. Further research incorporating therapies targeted toward improved cardiac relaxation (lusitropy) must be pursued.
In this preliminary study, we have found that after adjustment for severity of illness, cardiac disease, fluid management and grade of diastolic dysfunction, E/e' is an independent predictor of hospital survival in septic shock patients. In addition, E/e' offers better discrimination between hospital survivors and non-survivors than cardiac biomarkers (BNP, NTproBNP, TnT). Fluid balance and diastolic dysfunction are independent predictors of BNP concentration in septic shock.
E/e' is an independent predictor of hospital survival in septic shock patients.
E/e' offers better discrimination between hospital survivors and non-survivors than cardiac biomarkers (BNP, NTproBNP, TnT).
Fluid balance and diastolic dysfunction are independent predictors of BNP concentration in septic shock.
peak active (late) diastolic transmitral flow velocity
peak active (late) diastolic septal mitral annulus velocity
- APACHE III:
Acute Physiology and Chronic Health Evaluation III
B-type natriuretic peptide
body surface area
cardiac output indexed to body surface area
C reactive protein
central venous pressure
diastolic blood pressure
E wave deceleration time
peak early diastolic transmitral flow velocity
Peak early diastolic septal mitral annulus velocity
ratio of E to A
patio of E to e'
multiple-linear regression analyses of BNP concentration
left ventricle or ventricular
left ventricular end-diastolic volume
left ventricular end-systolic volume
LVEDV indexed to body surface area
LVESV indexed to body surface area
mean arterial pressure
LV outflow tract diameter
positive end expiratory pressure
receiver operating characteristic
peak systolic septal mitral annulus velocity
systolic blood pressure
Sequential Organ Failure Assessment score
left ventricular stroke volume indexed to body surface area
Tissue Doppler imaging
peak LV outflow tract velocity
LV outflow tract velocity time integral.
Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, Cohen J, Opal SM, Vincent JL, Ramsay G: 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Crit Care Med 2003, 31: 1250-1256. 10.1097/01.CCM.0000050454.01978.3B
Krishnagopalan S, Kumar A, Parrillo JE: Myocardial dysfunction in the patient with sepsis. Curr Opin Crit Care 2002, 8: 376-388. 10.1097/00075198-200210000-00003
Sturgess DJ, Marwick TH, Joyce CJ, Venkatesh B: B-Type Natriuretic Peptide Concentrations and Myocardial Dysfunction in Critical Illness. Anaesth Intensive Care 2006, 34: 151-163.
Parekh N, Maisel AS: Utility of B-natriuretic peptide in the evaluation of left ventricular diastolic function and diastolic heart failure. Curr Opin Cardiol 2009, 24: 155-160. 10.1097/HCO.0b013e328320d82a
Dahlstrom U: Can natriuretic peptides be used for the diagnosis of diastolic heart failure? Eur J Heart Fail 2004, 6: 281-287. 10.1016/j.ejheart.2004.01.005
Sturgess DJ, Marwick TH, Venkatesh B: Diastolic (Dys)Function in Sepsis. In Yearbook of Intensive Care and Emergency Medicine. Edited by: Vincent JL. Berlin Heidelberg: Springer-Verlag; 2007:444-454. full_text
Isaaz K, Thompson A, Ethevenot G, Cloez JL, Brembilla B, Pernot C: Doppler echocardiographic measurement of low velocity motion of the left ventricular posterior wall. Am J Cardiol 1989, 64: 66-75. 10.1016/0002-9149(89)90655-3
Dokainish H: Tissue Doppler imaging in the evaluation of left ventricular diastolic function. Curr Opin Cardiol 2004, 19: 437-441. 10.1097/01.hco.0000131538.55528.8f
Nagueh SF, Appleton CP, Gillebert TC, Marino PN, Oh JK, Smiseth OA, Waggoner AD, Flachskampf FA, Pellikka PA, Evangelisa A: Recommendations for the evaluation of left ventricular diastolic function by echocardiography. Eur J Echocardiogr 2009, 10: 165-193. 10.1093/ejechocard/jep007
Wang M, Yip GW, Wang AY, Zhang Y, Ho PY, Tse MK, Lam PK, Sanderson JE: Peak early diastolic mitral annulus velocity by tissue Doppler imaging adds independent and incremental prognostic value. J Am Coll Cardiol 2003, 41: 820-826. 10.1016/S0735-1097(02)02921-2
Hillis GS, Moller JE, Pellikka PA, Gersh BJ, Wright RS, Ommen SR, Reeder GS, Oh JK: Noninvasive estimation of left ventricular filling pressure by e/e' is a powerful predictor of survival after acute myocardial infarction. J Am Coll Cardiol 2004, 43: 360-367. 10.1016/j.jacc.2003.07.044
Moller JE, Sondergaard E, Poulsen SH, Seward JB, Appleton CP, Egstrup K: Color M-mode and pulsed wave tissue Doppler echocardiography: powerful predictors of cardiac events after first myocardial infarction. J Am Soc Echocardiogr 2001, 14: 757-763. 10.1067/mje.2001.113367
Troughton RW, Prior DL, Frampton CM, Nash PJ, Pereira JJ, Martin M, Fogarty A, Morehead AJ, Starling RC, Young JB, Thomas JD, Lauer MS, Klein AL: Usefulness of tissue doppler and color M-mode indexes of left ventricular diastolic function in predicting outcomes in systolic left ventricular heart failure (from the ADEPT study). Am J Cardiol 2005, 96: 257-262. 10.1016/j.amjcard.2005.03.055
Olson JM, Samad BA, Alam M: Prognostic value of pulse-wave tissue Doppler parameters in patients with systolic heart failure. Am J Cardiol 2008, 102: 722-725. 10.1016/j.amjcard.2008.04.054
Dini FL, Conti U, Fontanive P, Andreini D, Panicucci E, De Tommasi SM: Prognostic value of N-terminal pro-type-B natriuretic peptide and Doppler left ventricular diastolic variables in patients with chronic systolic heart failure stabilized by therapy. Am J Cardiol 2008, 102: 463-468. 10.1016/j.amjcard.2008.03.083
Dokainish H, Zoghbi WA, Lakkis NM, Ambriz E, Patel R, Quinones MA, Nagueh SF: Incremental predictive power of B-type natriuretic peptide and tissue Doppler echocardiography in the prognosis of patients with congestive heart failure. J Am Coll Cardiol 2005, 45: 1223-1226. 10.1016/j.jacc.2005.01.025
Marwick TH, Case C, Leano R, Short L, Baglin T, Cain P, Garrahy P: Use of tissue Doppler imaging to facilitate the prediction of events in patients with abnormal left ventricular function by dobutamine echocardiography. Am J Cardiol 2004, 93: 142-146. 10.1016/j.amjcard.2003.09.029
Okura H, Takada Y, Kubo T, Iwata K, Mizoguchi S, Taguchi H, Toda I, Yoshikawa J, Yoshida K: Tissue doppler derived index of left ventricular filling pressure, e/e', predicts survival in patients with non- valvular atrial fibrillation. Heart 2006.
Wang M, Yip GW, Wang AY, Zhang Y, Ho PY, Tse MK, Yu CM, Sanderson JE: Tissue Doppler imaging provides incremental prognostic value in patients with systemic hypertension and left ventricular hypertrophy. J Hypertens 2005, 23: 183-191. 10.1097/00004872-200501000-00029
Wang AY, Wang M, Lam CW, Chan IH, Zhang Y, Sanderson JE: Left ventricular filling pressure by Doppler echocardiography in patients with end-stage renal disease. Hypertension 2008, 52: 107-114. 10.1161/HYPERTENSIONAHA.108.112334
Sturgess DJ, Marwick TH, Joyce CJ, Jones M, Venkatesh B: Tissue Doppler in critical illness: a retrospective cohort study. Crit Care 2007, 11: R97. 10.1186/cc6114
Ikonomidis I, Nikolaou M, Dimopoulou I, Paraskevaidis I, Lekakis J, Mavrou I, Tzanela M, Kopterides P, Tsangaris I, Armaganidis A, Kremastinos DT: Association of left ventricular diastolic dysfunction with elevated NT-proBNP in general intensive care unit patients with preserved ejection fraction: A complementary role of Tissue Doppler imaging parameters and NT-proBNP levels for adverse outcome. Shock 2010, 33: 141-8. 10.1097/SHK.0b013e3181ad31f8
Charpentier J, Luyt CE, Fulla Y, Vinsonneau C, Cariou A, Grabar S, Dhainaut JF, Mira JP, Chiche JD: Brain natriuretic peptide: A marker of myocardial dysfunction and prognosis during severe sepsis. Crit Care Med 2004, 32: 660-665. 10.1097/01.CCM.0000114827.93410.D8
Post F, Weilemann LS, Messow CM, Sinning C, Munzel T: B-type natriuretic peptide as a marker for sepsis-induced myocardial depression in intensive care patients. Crit Care Med 2008, 36: 3030-3037. 10.1097/CCM.0b013e31818b9153
Varpula M, Pulkki K, Karlsson S, Ruokonen E, Pettila V: Predictive value of N-terminal pro-brain natriuretic peptide in severe sepsis and septic shock. Crit Care Med 2007, 35: 1277-1283. 10.1097/01.CCM.0000261893.72811.0F
Spies C, Haude V, Fitzner R, Schroder K, Overbeck M, Runkel N, Schaffartzik W: Serum cardiac troponin T as a prognostic marker in early sepsis. Chest 1998, 113: 1055-1063. 10.1378/chest.113.4.1055
Dellinger RP, Levy MM, Carlet JM, Bion J, Parker MM, Jaeschke R, Reinhart K, Angus DC, Brun-Buisson C, Beale R, Calandra T, Dhainaut JF, Gerlach H, Harvey M, Marini JJ, Marshall J, Ranieri M, Ramsay G, Sevransky J, Thompson BT, Townsend S, Vender JS, Zimmerman JL, Vincent JL, International Surviving Sepsis Campaign Guidelines Committee; American Association of Critical-Care Nurses; American College of Chest Physicians; American College of Emergency Physicians; Canadian Critical Care Society; European Society of Clinical Microbiology and Infectious Diseases, et al.: Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock: 2008. Crit Care Med 2008, 36: 296-327. 10.1097/01.CCM.0000298158.12101.41
Mosteller RD: Simplified calculation of body-surface area. N Engl J Med 1987, 317: 1098.
Lang RM, Bierig M, Devereux RB, Flachskampf FA, Foster E, Pellikka PA, Picard MH, Roman MJ, Seward J, Shanewise JS, Solomon SD, Spencer KT, Sutton MS, Stewart WJ, Chamber Quantification Writing Group; American Society of Echocardiography's Guidelines and Standards Committee; European Association of Echocardiography: Recommendations for chamber quantification: a report from the American Society of Echocardiography's Guidelines and Standards Committee and the Chamber Quantification Writing Group, developed in conjunction with the European Association of Echocardiography, a branch of the European Society of Cardiology. J Am Soc Echocardiogr 2005, 18: 1440-1463. 10.1016/j.echo.2005.10.005
Quinones MA, Otto CM, Stoddard M, Waggoner A, Zoghbi WA: Recommendations for quantification of Doppler echocardiography: a report from the Doppler Quantification Task Force of the Nomenclature and Standards Committee of the American Society of Echocardiography. J Am Soc Echocardiogr 2002, 15: 167-184. 10.1067/mje.2002.120202
Nagueh SF, Mikati I, Kopelen HA, Middleton KJ, Quinones MA, Zoghbi WA: Doppler estimation of left ventricular filling pressure in sinus tachycardia. A new application of tissue doppler imaging. Circulation 1998, 98: 1644-1650.
Garcia-Fernandez MA, Azevedo J, Moreno M, Bermejo J, Perez-Castellano N, Puerta P, Desco M, Antoranz C, Serrano JA, Garcia E, Delcan JL: Regional diastolic function in ischaemic heart disease using pulsed wave Doppler tissue imaging. Eur Heart J 1999, 20: 496-505. 10.1053/euhj.1998.1278
Ommen SR, Nishimura RA, Appleton CP, Miller FA, Oh JK, Redfield MM, Tajik AJ: Clinical utility of Doppler echocardiography and tissue Doppler imaging in the estimation of left ventricular filling pressures: A comparative simultaneous Doppler-catheterization study. Circulation 2000, 102: 1788-1794.
Mottram PM, Marwick TH: Assessment of diastolic function: what the general cardiologist needs to know. Heart 2005, 91: 681-695. 10.1136/hrt.2003.029413
McLean AS, Huang SJ, Nalos M, Tang B, Stewart DE: The confounding effects of age, gender, serum creatinine, and electrolyte concentrations on plasma B-type natriuretic peptide concentrations in critically ill patients. Crit Care Med 2003, 31: 2611-2618. 10.1097/01.CCM.0000094225.18237.20
Inoha S, Inamura T, Nakamizo A, Matushima T, Ikezaki K, Fukui M: Fluid loading in rats increases serum brain natriuretic peptide concentration. Neurol Res 2001, 23: 93-95. 10.1179/016164101101198190
Hanford DS, Glembotski CC: Stabilization of the B-type natriuretic peptide mRNA in cardiac myocytes by alpha-adrenergic receptor activation: potential roles for protein kinase C and mitogen-activated protein kinase. Mol Endocrinol 1996, 10: 1719-1727. 10.1210/me.10.12.1719
Shor R, Rozenman Y, Bolshinsky A, Harpaz D, Tilis Y, Matas Z, Fux A, Boaz M, Halabe A: BNP in septic patients without systolic myocardial dysfunction. Eur J Intern Med 2006, 17: 536-540. 10.1016/j.ejim.2006.07.013
McDonagh TA, Robb SD, Murdoch DR, Morton JJ, Ford I, Morrison CE, Tunstall-Pedoe H, McMurray JJ, Dargie HJ: Biochemical detection of left-ventricular systolic dysfunction. Lancet 1998, 351: 9-13. 10.1016/S0140-6736(97)03034-1
Lubien E, DeMaria A, Krishnaswamy P, Clopton P, Koon J, Kazanegra R, Gardetto N, Wanner E, Maisel AS: Utility of B-natriuretic peptide in detecting diastolic dysfunction: comparison with Doppler velocity recordings. Circulation 2002, 105: 595-601. 10.1161/hc0502.103010
Dupont WD, Plummer WD: PS power and sample size program available for free on the internet. Controlled Clin Trials 1997, 18: 274. 10.1016/S0197-2456(97)00074-3
Yu CM, Sanderson JE, Marwick TH, Oh JK: Tissue Doppler imaging a new prognosticator for cardiovascular diseases. J Am Coll Cardiol 2007, 49: 1903-1914. 10.1016/j.jacc.2007.01.078
Parker MM, Shelhamer JH, Bacharach SL, Green MV, Natanson C, Frederick TM, Damske BA, Parrillo JE: Profound but reversible myocardial depression in patients with septic shock. Ann Intern Med 1984, 100: 483-490.
Parrillo JE: Pathogenetic mechanisms of septic shock. N Engl J Med 1993, 328: 1471-1477. 10.1056/NEJM199305203282008
Munt B, Jue J, Gin K, Fenwick J, Tweeddale M: Diastolic filling in human severe sepsis: an echocardiographic study. Crit Care Med 1998, 26: 1829-1833.
Fernandes CJ Junior, Akamine N, Knobel E: Myocardial Depression in Sepsis. Shock 2008, 30: 14-17. 10.1097/SHK.0b013e3181818617
Rudiger A, Singer M: Mechanisms of sepsis-induced cardiac dysfunction. Crit Care Med 2007, 35: 1599-1608. 10.1097/01.CCM.0000266683.64081.02
Sohn DW, Chai IH, Lee DJ, Kim HC, Kim HS, Oh BH, Lee MM, Park YB, Choi YS, Seo JD, Lee YW: Assessment of mitral annulus velocity by Doppler tissue imaging in the evaluation of left ventricular diastolic function. J Am Coll Cardiol 1997, 30: 474-480. 10.1016/S0735-1097(97)88335-0
Vignon P, Allot V, Lesage J, Martaille JF, Aldigier JC, Francois B, Gastinne H: Diagnosis of left ventricular diastolic dysfunction in the setting of acute changes in loading conditions. Crit Care 2007, 11: R43. 10.1186/cc5736
Jacques DC, Pinsky MR, Severyn D, Gorcsan J: Influence of alterations in loading on mitral annular velocity by tissue Doppler echocardiography and its associated ability to predict filling pressures. Chest 2004, 126: 1910-1918. 10.1378/chest.126.6.1910
Marwick TH: Clinical applications of tissue Doppler imaging: a promise fulfilled. Heart 2003, 89: 1377-1378. 10.1136/heart.89.12.1377
Nagueh SF, Middleton KJ, Kopelen HA, Zoghbi WA, Quinones MA: Doppler tissue imaging: a noninvasive technique for evaluation of left ventricular relaxation and estimation of filling pressures. J Am Coll Cardiol 1997, 30: 1527-1533. 10.1016/S0735-1097(97)00344-6
McLean AS, Huang SJ, Hyams S, Poh G, Nalos M, Pandit R, Balik M, Tang B, Seppelt I: Prognostic values of B-type natriuretic peptide in severe sepsis and septic shock. Crit Care Med 2007, 35: 1019-1026. 10.1097/01.CCM.0000259469.24364.31
Rivers E, Nguyen B, Havstad S, Ressler J, Muzzin A, Knoblich B, Peterson E, Tomlanovich M: Early goal-directed therapy in the treatment of severe sepsis and septic shock. N Engl J Med 2001, 345: 1368-1377. 10.1056/NEJMoa010307
Bouhemad B, Nicolas-Robin A, Arbelot C, Arthaud M, Feger F, Rouby JJ: Isolated and reversible impairment of ventricular relaxation in patients with septic shock. Crit Care Med 2008, 36: 766-774. 10.1097/CCM.0B013E31816596BC
Maisel AS, Krishnaswamy P, Nowak RM, McCord J, Hollander JE, Duc P, Omland T, Storrow AB, Abraham WT, Wu AH, Clopton P, Steg PG, Westheim A, Knudsen CW, Perez A, Kazanegra R, Herrmann HC, McCullough PA, Breathing Not Properly Multinational Study Investigators: Rapid measurement of B-type natriuretic peptide in the emergency diagnosis of heart failure. N Engl J Med 2002, 347: 161-167. 10.1056/NEJMoa020233
Pirracchio R, Deye N, Lukaszewicz AC, Mebazaa A, Cholley B, Mateo J, Megarbane B, Launay JM, Peynet J, Baud F, Payen D: Impaired plasma B-type natriuretic peptide clearance in human septic shock. Crit Care Med 2008, 36: 2542-2546. 10.1097/CCM.0b013e318183f067
Shirakami G, Magaribuchi T, Shingu K, Suga S, Tamai S, Nakao K, Mori K: Positive end-expiratory pressure ventilation decreases plasma atrial and brain natriuretic peptide levels in humans. Anesth Analg 1993, 77: 1116-1121. 10.1213/00000539-199312000-00006
McLean AS, Poh G, Huang SJ: The effects of acute fluid loading on plasma B-type natriuretic peptide levels in a septic shock patient. Anaesth Intensive Care 2005, 33: 528-530.
Sturgess DJ, Pascoe RLS, Scalia G, Venkatesh B: A Comparison of Transcutaneous Doppler corrected flow time, b-type natriuretic peptide and central venous pressure as predictors of fluid responsiveness in septic shock: a preliminary evaluation. Anaesth Intensive Care 2010, 38: 336-341.
Antonelli M, Levy M, Andrews PJ, Chastre J, Hudson LD, Manthous C, Meduri GU, Moreno RP, Putensen C, Stewart T, Torres A: Hemodynamic monitoring in shock and implications for management: International Consensus Conference, Paris, France, 27-28 April 2006. Intensive Care Med 2007, 33: 575-590. 10.1007/s00134-007-0531-4
Mr Goce Dimeski (Chemical Pathology, Princess Alexandra Hospital, Queensland Health Pathology Service, Ipswich Road, Brisbane, Australia) assisted with advice regarding biochemical assay techniques. Dr Elaine Beller (School of Population Health, The University of Queensland, Princess Alexandra Hospital, Brisbane, Australia) assisted with advice regarding final statistical analysis. This study was conducted with the support of grants from the Australian and New Zealand College of Anaesthetists. This study was performed at the Department of Intensive Care and The University of Queensland, Princess Alexandra Hospital, Ipswich Road, Brisbane, 4102, Australia.
The authors declare that they have no competing interests.
D Sturgess conceived of the study, coordinated study design and implementation and drafted the manuscript. TM participated in study design and helped to draft the manuscript. C Joyce participated in study design and helped to draft the manuscript. C Jenkins participated in the design of the study, performed and coordinated echocardiography. MJ participated in the design of the study and provided statistical advise. PM participated in study design, provided laboratory equipment and advice regarding biochemical assays. D Stewart assisted in recruitment of participants and collection of data. BV participated in its design and helped to draft the manuscript. All authors read and approved the final manuscript.
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Sturgess, D.J., Marwick, T.H., Joyce, C. et al. Prediction of hospital outcome in septic shock: a prospective comparison of tissue Doppler and cardiac biomarkers. Crit Care 14, R44 (2010). https://doi.org/10.1186/cc8931
- Septic Shock
- Mean Arterial Pressure
- Diastolic Dysfunction
- Diastolic Function
- Fluid Balance