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Microvascular permeability during experimental human endotoxemia: an open intervention study

Critical Care volume 9, Article number: R157 (2005) Cite this article

Abstract

Introduction

Septic shock is associated with increased microvascular permeability. As a model for study of the pathophysiology of sepsis, endotoxin administration to humans has facilitated research into inflammation, coagulation and cardiovascular effects. The present study was undertaken to determine whether endotoxin administration to human volunteers can be used as a model to study the sepsis-associated increase in microvascular permeability.

Methods

In an open intervention study conducted in a university medical centre, 16 healthy volunteers were evaluated in the research unit of the intensive care unit. Eight were administered endotoxin intravenously (2 ng/kg Escherichia coli O113) and eight served as control individuals. Microvascular permeability was assessed before and 5 hours after the administration of endotoxin (n = 8) or placebo (n = 8) by three different methods: transcapillary escape rate of I125-albumin; venous occlusion strain-gauge plethysmography to determine the filtration capacity; and bioelectrical impedance analysis to determine the extracellular and total body water.

Results

Administration of endotoxin resulted in the expected increases in proinflammatory cytokines, temperature, flu-like symptoms and cardiovascular changes. All changes were significantly different from those in the control group. In the endotoxin group all microvascular permeability parameters remained unchanged from baseline: transcapillary escape rate of I125-albumin changed from 7.2 ± 0.6 to 7.7 ± 0.9%/hour; filtration capacity changed from 5.0 ± 0.3 to 4.2 ± 0.4 ml/min per 100 ml mmHg × 10-3; and extracellular/total body water changed from 0.42 ± 0.01 to 0.40 ± 0.01 l/l (all differences not significant).

Conclusion

Although experimental human endotoxaemia is frequently used as a model to study sepsis-associated pathophysiology, an endotoxin-induced increase in microvascular permeability in vivo could not be detected using three different methods. Endotoxin administration to human volunteers is not suitable as a model in which to study changes in microvascular permeability.

Introduction

Sepsis is the leading cause of mortality in noncardiac intensive care units, resulting in an estimated mortality of 200,000 patients per year in the USA alone [1]. Sepsis is notably characterized by an increase in microvascular permeability, which accounts for the extravasation of macromolecules and fluid from the plasma to the tissues. The impaired diffusion of oxygen to cells as a result of the extracellular oedema appears to be a critical factor in the development of multiple organ failure [2, 3]. Few studies have been conducted in humans to examine the mechanism that underlies the sepsis-associated increase in microvascular permeability.

Endotoxin is among the principal bacterial components that interacts with the host during Gram-negative sepsis [4]. Administration of endotoxin to humans is an appropriate model in which to investigate acute inflammatory responses (activation of cytokines and coagulation pathways) and to evaluate novel therapeutic interventions [5]. In vitro, exposure of human endothelial cells to endotoxin induces an increase in permeability [6], and in vivo an increase in microvascular permeability is among the major manifestations observed in animal endotoxaemia [7–12]. In humans, microvascular permeability can be assessed by plasma disappearance of a tracer (e.g. I125-albumin), changes in tissue volume caused by an imposed hydrostatic pressure and changes in bio-impedance. These methods were validated for the detection of a modest increase in microvascular permeability in patients with various diseases [13–17] and, more relevant to our study, patients with sepsis or septic shock [18–20]. In septic patients, transcapillary escape rate of albumin varies from 6.7%/hour [21] to 13.4%/hour [18], whereas permeability measured using venous congestion plethysmography (VCP) ranged from 6.1 ml/min per 100 ml mmHg × 10-3 [19] to 9.3 ml/min per 100 ml mmHg × 10-3 [22].

The present study was undertaken to determine whether endotoxin administration to human volunteers can be employed as a model in which to study the sepsis-associated increase in microvascular permeability.

Materials and methods

Subjects

After approval had been granted by the local ethics committee, 16 nonsmoking individuals gave written informed consent to participate in the study. Those who were taking prescription drugs or asprin or other nonsteroidal anti-inflammatory drugs were excluded (except for oral anticontraceptives). Screening of the participants before the test revealed no abnormalities in medical history or physical examination. Routine laboratory tests and electrocardiograms were normal. All participants were HIV and hepatitis B negative. They had not suffered a febrile illness within the 2 weeks preceding the study. Ten hours before the experiment, the participants refrained from consuming caffeine, alcohol and food.

Study design and procedures

Heart rate was continuously monitored using a three-lead electrogradiograph. An intra-arterial catheter in the radial artery permitted arterial blood sampling as well as continuous monitoring of blood pressure throughout the experiment. Forearm blood flow was measured in both arms using VCP, as described previously [23]. All participant received an intravenous infusion of a glucose/saline solution (2.5% glucose, 0.45% saline; 75 cm3/hour) via a cannula in an antecubital vein. At baseline, purified lipopolysaccharide (LPS) prepared from Escherichia coli O113 was injected intravenously (2 ng/kg) over 1–2 min in eight individuals, followed by 5 ml normal saline to ensure complete delivery. Another eight served as control individuals and received NaCl 0.9% instead of endotoxin in an equivalent volume. Because of obvious symptomatic changes after infusion of endotoxin, neither the volunteers nor the staff members were blinded to the study protocol.

The course over time of temperature, C-reactive protein, and plasma levels of tumour necrosis factor (TNF)-α and interleukin (IL)-1β [24] were monitored to confirm the inflammatory effects of endotoxin administration.

Transcapillary escape rate of I125albumin

Microvascular permeability determined by the transcapillary escape rate of I125-albumin (TER-alb) was measured at baseline and 5 hours after endotoxin administration, when haemodynamic changes are at their maximum [25]. I125 labelled albumin solution of 2 μCi (baseline) and 6 μCi (at 5 hours) in 5 cm3 normal saline were given as an intravenous bolus injection followed by 5 cm3 normal saline. The second dose is higher to overcome the background signal of the first dose. Arterial blood samples were drawn at baseline, and at 5, 10, 15, 20, 30, 45 and 60 min. Plasma radioactivity was measured in each sample using a scintillation detector (automatic γ-counter; 1480 Wizard 3", Wallac, Turku, Finland).

Venous congestion plethysmography

Microvascular permeability was also determined by VCP, in accordance with methods fully described previously [26, 27]. Microvascular filtration capacity (Kf) – an index of vascular permeability – was measured using a protocol in which a series of eight small (10 mmHg) cumulative pressure steps were applied to venous congestion cuffs placed around both upper arms. Kf was estimated from alterations in forearm circumference due to the pressures applied, using the Filtrass strain gauge plethysmograph (Filtrass Angio, DOMED, Munich, Germany) [27]. Using this system, no change in the recorded signal is observed until ambient venous pressure in the arm is exceeded. At congestion cuff pressures greater than this value, each additional pressure increment causes a change in forearm volume that is attributed to vascular filling. When the congestion cuff pressure exceeds the isovolumetric venous pressure, a steady state change in volume is observed, reflecting fluid filtration. Kf reflects the product of the area available for fluid filtration and the permeability per unit surface area. Computer-based analysis enables differentiation between volume and filtration responses [28]. The value of Kf is determined by linear regression of the fluid filtration as a function of the cuff pressure. The slope of this relationship is Kf and the units are expressed as KfU (ml/min per 100 ml mmHg × 10-3) [28]. The files were recorded and saved for subsequent offline analysis. Kf measurements were conducted before, and 4.5 hours and 22 hours after the administration of endotoxin or normal saline.

Bioelectrical impedance analysis

In septic patients, fluid shifts from intracellular water to extracellular water (ECW) and an increase in total body water (TBW) occur because of an altered cellular membrane function, resulting in the formation of oedema. Bioelectrical impedance analysis (BIA) can estimate body composition parameters and has been used to estimate body water distribution and cellular membrane function in healthy individuals [29] and intensive care patients [20, 30–33]. The principles of bioelectrical impedance postulate that resistance (R) is the opposition of TBW and electrolytes to the flow of an alternating current of low amplitude (800 μA) and high frequency (50 kHz). Reactance is the capacitance produced by tissue interfaces and cell membranes. An increase in microvascular permeability and an altered membrane function result in the formation of oedema, which decreases the resistance and reactance to an alternating electric current throughout the body. ECW will increase in relation to TBW, and reactance/resistance will decrease. BIA was performed using a body composition analyzer (Akern Srl, Florence, Italy). This device employs four-electrode polarization and measures the resistance and reactance of a conductor to application of an alternating electric current of 800 μA and 50 kHz. All measurements were made with the patient supine, with their arms relaxed at their sides but not touching their body, and with their thighs slightly separated. Electrodes were placed on the dorsal surface of the skin of the wrist and ankle, with the detector electrodes applied along the articulation bisecting line of both joints. BIA was performed at baseline and 4, 6, 8 and 22 hours after endotoxin administration.

Drugs and solutions

All solutions were freshly prepared on the day of the experiment. Endotoxin from Escherichia coli (batch 0:113, lot G2B274) was obtained from US Pharmacopia Convention (Rockville, MD, USA) and dissolved in normal saline 0.9% to a concentration of 200 EU/ml (0.1 ml/kg). I125-albumin (Iodinated [125I] Human Serum Albumin; code IM 17 P) was obtained from Amersham International (Amersham, UK).

Data analysis, calculations and statistics

Power analysis was based on clinically relevant changes in TER-Alb. In a previous study using the TER-alb method, we found a standard deviation ranging from 1.5% to 2.5%. An increase in transcapillary escape rate of 2.5% was considered clinically relevant. With an estimated standard deviation of 2% and α = 0.05, we calculated that a sample size of seven individuals per group would be needed to achieve a power of 95%. Therefore, eight individuals per group were included.

TER-alb was calculated and expressed as the percentage disappearance per hour. Fluid filtration capacity (Kf) was determined by venous occlusion plethysmography in both forearms and averaged. The mean Kf was used for further calculations. A change in the ratio of ECW/TBW was taken to give an impression of microvascular permeability, using BIA.

Student's t-tests or analysis of variance with repeated measures were used for the assessment of the effects of endotoxin on microvascular permeability parameters. All data are expressed as mean ± standard error of the mean of n experiments unless otherwise stated. P < 0.05 was considered statistically significant.

Results

Demographic characteristics of the participants are presented in Table 1. There were no significant differences between the groups.

Table 1 Demographic characteristics of the participants
Full size table

Changes in clinical and inflammatory parameters

The first flu-like symptoms (headache, nausea, chills) occurred in the endotoxin-treated group between 55 and 90 min after LPS injection. Body temperature started to rise 1 hour after endotoxin administration to a maximum of 38.7 ± 0.3°C at 4 hours versus 36.9 ± 0.2°C in the control group (P < 0.001). At 8 hours all clinical symptoms had declined to control values. The clinical onset of inflammation was accompanied by a sudden rise in TNF-α plasma levels at 60 min (373 ± 71 pg/ml), which reached its zenith at 90 min (856 ± 158 pg/ml), closely followed by a rise in IL-1β that was maximal at 120 min (23.9 ± 2.2 pg/ml). C-reactive protein increased from under 5 mg/ml at baseline to 22.3 ± 1.4 mg/ml at 12 hours after endotoxin administration and reached its maximum at 22 hours (38.9 ± 3.0 mg/ml). In the control individuals no elevations in temperature (from 36.9 ± 0.1 to 37.0 ± 0.1°C), clinical symptoms, cytokine levels (TNF-α <8 pg/ml, IL-1β <8 pg/ml) or C-reative protein (<5 mg/ml) were observed (Fig. 1).

Figure 1
figure 1

Changes in inflammatory parameters. Administration of endotoxin (n = 8; 2 ng/kg) resulted in a marked increase in tumour necrosis factor (TNF)-α (closed squares, left axis) and IL-1β (open squares, right axis). In control individuals cytokine levels remained below the detection limit (n = 8; data not shown). Cytokine release was associated with fever and an increase in C-reactive protein (CRP; endotoxin group, closed squares; control group, open circles). Data are expressed as means ± standard error of the mean. The P values in the figure refer to the difference between endotoxin and control groups as analyzed using analysis of variance with repeated measures over the complete curve.

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Changes in haemodynamic parameters

Figure 2 shows the course of heart rate, mean arterial pressure and forearm blood flow in the endotoxin and control group. In the control group the mean arterial blood pressure decreased from 88 to 80 mmHg at 6 hours (P = 0.035); the blood pressure decreased significantly more in the individuals administered LPS (from 96 ± 3 mmHg to 79 ± 4 mmHg at 6 hours, P < 0.0001; difference from control individuals: P = 0.002). Heart rate remained unchanged in the control group (from 66 ± 4 to 65 ± 2 beats/min; not significant) and increased from 63 ± 3 to 91 ± 3 beats/min at 6 hours in the LPS group (P < 0.0001). Forearm blood flow increased from 3.7 ± 0.6 to 6.8 ± 1.1 ml/min per dl at 6 hours (P = 0.018) in the endotoxin group, but remained unchanged in the control group (3.8 ± 0.8 versus 4.4 ± 0.9 ml/min per dl; not significant).

Figure 2
figure 2

Changes in haemodynamic parameters. Administration of endotoxin (2 ng/kg; n = 8; closed squares) resulted in a significant increase in heart rate (HR; measured using electrocardiography; P < 0.0001), a significant decrease in mean arterial pressure (MAP; measured intra-arterially; P < 0.0001) and a significant increase in forearm blood flow (FBF; measured using venous occlusion plethysmography; P = 0.018). HR and FBF did not change significantly in the control group (open circles; n = 8), whereas MAP decreased (P = 0.035). MAP decreased significantly more in the endotoxin group than in the control group (P = 0.002). These changes demonstrate that endotoxin induces a vasodilatory state. Data are expressed as means ± standard error of the mean. The P values in the figure refer to the difference between endotoxin and control group as analyzed using analysis of variance with repeated measures over the complete curve.

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Changes in microvascular permeability parameters

In neither the endotoxin group nor the control group were significant alterations in microvascular permeability parameters detected. In the endotoxin group TER-alb was 7.2 ± 0.6%/hour before and 7.7 ± 0.9%/hour at 4.5 hours after endotoxin administration (not significant); Kf remained unchanged (from 5.0 ± 0.3 to 4.2 ± 0.4 ml/min per 100 ml mmHg × 10-3; not significant); and ECW/TBW, as measured by BIA, did not change (from 0.42 ± 0.01 l/l to 0.40 ± 0.01 l/l; not significant). Also, no significant changes in microvascular permeability parameters were found in the control group (all not significant: TER-alb from 9.08 ± 1.28 to 10.38 ± 0.63%/hour; Kf from 4.14 ± 0.42 to 5.17 ± 0.39 ml/min per 100 ml mmHg × 10-3; and ECW/TBW from 0.43 ± 0.01 l/l to 0.42 ± 0.01 l/l). The effect of endotoxin on microvascular parameters is shown in Fig. 3.

Figure 3
figure 3

Changes in microvascular permeability parameters. Microvascular permeability parameters were measured using transcapillary escape rate of I125-albumin (TER-alb), venous congestion plethysmography (VCP) and bioelectrical impedance analysis (BIA). There were no changes in microvascular permeability as measured using all three parameters in either the endotoxin group (n = 8; 2 ng/kg; closed squares) or in the control group (n = 8; open circles). Data are expressed as means ± standard error of the mean. ECW, extracellular water; TBW, total body water.

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Discussion

Although administration of endotoxin to human volunteers has facilitated sepsis-associated research, the present study demonstrates that human experimental endotoxaemia is not a suitable model in which to study sepsis-induced changes in microvascular permeability. In a negative study the first issue to address is methodology. We conducted the present study with all three methods that are available for human in vivo experiments. Differences in microvascular permeability have been detected in various other diseases with these methods [13–17]. In septic patients an increase in Kf was demonstrated with TER-alb [18], VCP [19] and BIA [20]. In view of the ability of these methods to detect differences in microvascular permeability and the consistently negative findings of all three methods used in this endotoxin study, we believe our results are valid.

There are several possible reasons for our negative findings. First, the inflammatory stimulus might not have been sufficiently powerful. Endotoxin is known to stimulate the immune system in a dose-dependent manner [25]. Indeed, a marked increase in permeability in vivo has previously been shown in, for example, cats after intravenous administration of 1 mg/kg endotoxin [10]. On one occasion, an autointoxication with 1 mg of Salmonella endotoxin resulted in profound vasodilatory shock and a 15 l cumulative fluid balance over 72 hours in a laboratory worker [34]. This demonstrates unequivocally that high doses of endotoxin can cause shock and vascular leakage. In human volunteers an endotoxin concentration of 4 ng/kg is considered the maximal tolerable dose. The concentration of 2 ng/kg is widely applied and results in systemic inflammation, activation of coagulation pathways and distinct haemodynamic changes. Although the rise in proinflammatory cytokines is dose dependent, studies that used 4 ng/kg LPS found changes in clinical parameters similar to those reported here (e.g. rise in body temperature and fall in blood pressure) [35]. In the individuals included in the present study (who received 2 ng/kg) the flu-like symptoms, rise in body temperature, rise in heart rate, fall in blood pressure and rise in C-reactive protein were considerable; we therefore believe that the inflammatory stimulus was adequate. Also, the TNF-α and IL-1β concentrations in these individuals exceeded considerably the threshold levels of 50 pg/ml and 20 pg/ml, respectively, that are necessary to increase permeability significantly in vitro [6].

Naturally, it remains difficult for many reasons to compare an in vitro study in endothelial cells of large vessels with our in vivo experiment. The human endotoxaemia model is currently the only available in vivo human model that mimics Gram-negative sepsis. Whereas in experimental endotoxaemia the stimulus is restricted to LPS, other (non-LPS) bacterial components are also of importance for the induction of cytokines and the inflammatory response [36] and possibly the induction of vascular leakage. These differences could represent the reason why therapies directed at endotoxaemia itself are not of benefit in patients with septic shock [37]. However, as a model, the changes in haemodynamics that occur during human endotoxaemia are similar to those observed in septic shock, and suggest that endotoxin is a major mediator of the cardiovascular dysfunction that occurs in this condition [35].

A second possible reason for our negative findings is that not only the peak concentration of cytokines but also the duration of the increased level of the inflammatory mediators may be important in the pathophysiology of oedema formation in sepsis. The stimulus caused by a single bolus injection of endotoxin may be too short to induce an increase in microvascular permeability. The induction of capillary leakage in vitro was accomplished after incubation with endotoxin or cytokines for 6 hours [6]. Also, in pre-eclampsia a sustained rise in plasma cytokines is associated with an increase in microvascular permeability, suggesting a causal relationship [38]. However, although in some cases of sepsis in humans (e.g. meningococcal disease) elevated serum levels of TNF-α have been found in up to 90% of patients [39], several other clinical studies in septic patients reported only minimally elevated or undetectable levels of TNF-α [40, 41]. Because these patients exhibit an overt increase in microvascular permeability, sustained high cytokine levels are apparently not mandatory for the development of oedema.

A third reason is that the timing of the measurements might not have been optimal for the detection of changes in permeability. In previous studies maximal changes in haemodynamic parameters were found between 2 and 6 hours after administration of endotoxin [35]. Because these vascular changes can partly be accounted for by endothelial dysfunction [42], we opted to measure microvascular permeability in the same time window. The possibility that an increase in permeability occurred outside the time window of interest appears unlikely because BIA was unchanged at five time points during the experiment, and Kf was also unaltered at 22 hours after endotoxin administration. Timing may be of critical importance because an accelerated plasma efflux of albumin was only observed during the early phase of sepsis in rats [43]. Also, late-acting cytokines (e.g. high mobility group protein 1) remain elevated for 16–32 hours after the administration of endotoxin and may play a role in the capillary leak found in septic patients. This and possibly other mediators were not measured during our experiment. Again, BIA and VCP measurements after 22 hours did not reveal an increase in vascular permeability in our experiments, suggesting that a possible late increase in vascular permeability was not missed.

Finally, oedema formation may differ from tissue to tissue and from organ to organ. In human endotoxaemia increases in intestinal permeability [44] and alveolar epithelial permeability [45] were previously demonstrated. In contrast, human endotoxaemia did not induce an increase in the ocular blood–aqueous barrier [46]. With the TER-alb and BIA whole body permeability is assessed, whereas the Filtrass strain gauge plethysmograph focuses on the forearms. An increase in microvascular permeability in, for example, the lungs was not specifically assessed, but if it was present it was insufficient to affect whole body permeability. Administration of iodated albumin as a measure of capillary leak may vary with hydration status, and albumin molecules might be too large to be useful as a sensitive permeability marker. However, these problems are overcome with the use of VCP. We believe that fluid loading would not have altered transcappilary leakage, because with the VCP method a venous occlusion pressure is applied to the forearms, so that vascular permeability is measured independent of the volume status of the subject. The suggestion that permeability might have been increased for smaller molecules than albumin can be ruled out for the same reason.

In summary, we do not believe that the methods used, the timing of the permeability measurements, or the absolute maximal cytokine concentrations can account for the observed lack of effect of endotoxin on microvascular permeability in humans. However, the short duration of cytokine increase possibly played a role.

Conclusion

Although endotoxin administration to humans has proven to be a valuable model for studying systemic inflammation and coagulation, this model cannot be used to investigate the pathophysiological mechanisms that underlie capillary leakage in sepsis or to evaluate pharmacological interventions aimed at attenuating the increase in microvascular permeability.

Key messages

  • Endotoxin administration to humans is a valuable model in which to investigate inflammatory and haemodynamic mechanisms in sepsis.

  • Endotoxin administration to humans does not affect microvascular permeability measured using TER-alb, VCP and BIA.

  • Endotoxin administration can not be used as a model to study the pathopysiological mechanisms that underlie capillary leakage in sepsis, or to evaluate the pharmacological interventions aimed at restoring normal microvascular permeability.

Abbreviations

BIA:

= bioelectrical impedance analysis

ECW:

= extracellular water

IL:

= interleukin

KfKf:

= filtration capacity

LPS:

= lipopolysaccharide

TER-alb:

= transcapillary escape rate of I125-albumin

TNF:

= tumour necrosis factor

TBW:

= total body water

VCP:

= venous congestion plethysmography.

References

  1. Angus DC, Linde-Zwirble WT, Lidicker J, Clermont G, Carcillo J, Pinsky MR: Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care. Crit Care Med 2001, 29: 1303-1310. 10.1097/00003246-200107000-00002

    Article  CAS  PubMed  Google Scholar 

  2. Fietsam R Jr, Villalba M, Glover JL, Clark K: Intra-abdominal compartment syndrome as a complication of ruptured abdominal aortic aneurysm repair. Am Surg 1989, 55: 396-402.

    PubMed  Google Scholar 

  3. Stone HH, Fulenwider JT: Renal decapsulation in the prevention of post-ischemic oliguria. Ann Surg 1977, 186: 343-355.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. van Deventer SJ, Buller HR, ten Cate JW, Sturk A, Pauw W: Endotoxaemia: an early predictor of septicaemia in febrile patients. Lancet 1988, 1: 605-609. 10.1016/S0140-6736(88)91412-2

    Article  CAS  PubMed  Google Scholar 

  5. Martich GD, Boujoukos AJ, Suffredini AF: Response of man to endotoxin. Immunobiology 1993, 187: 403-416.

    Article  CAS  PubMed  Google Scholar 

  6. Nooteboom A, Van Der Linden CJ, Hendriks T: Tumor necrosis factor-alpha and interleukin-1beta mediate endothelial permeability induced by lipopolysaccharide-stimulated whole blood. Crit Care Med 2002, 30: 2063-2068. 10.1097/00003246-200209000-00019

    Article  CAS  PubMed  Google Scholar 

  7. Balsa D, Merlos M, Giral M, Ferrando R, Garcia-Rafanell J, Forn J: Effect of endotoxin and platelet-activating factor on rat vascular permeability: role of vasoactive mediators. J Lipid Mediat Cell Signal 1997, 17: 31-45. 10.1016/S0929-7855(97)00019-9

    Article  CAS  PubMed  Google Scholar 

  8. Green K, Paterson CA, Cheeks L, Slagle T, Jay WM, Aziz MZ: Ocular blood flow and vascular permeability in endotoxin-induced inflammation. Ophthalmic Res 1990, 22: 287-294.

    Article  CAS  PubMed  Google Scholar 

  9. Hinder F, Booke M, Traber LD, Traber DL: Nitric oxide and endothelial permeability. J Appl Physiol 1997, 83: 1941-1946.

    CAS  PubMed  Google Scholar 

  10. Holbeck S, Grande PO: Endotoxin increases both protein and fluid microvascular permeability in cat skeletal muscle. Crit Care Med 2003, 31: 560-565. 10.1097/01.CCM.0000048620.88344.70

    Article  CAS  PubMed  Google Scholar 

  11. Laszlo F, Whittle BJ, Evans SM, Moncada S: Association of microvascular leakage with induction of nitric oxide synthase: effects of nitric oxide synthase inhibitors in various organs. Eur J Pharmacol 1995, 283: 47-53. 10.1016/0014-2999(95)00281-O

    Article  CAS  PubMed  Google Scholar 

  12. Sakai I, Ishihara H, Iwakawa T, Suzuki A, Matsuki A: Ratio of indocyanine green and glucose dilutions detects capillary protein leakage following endotoxin injection in dogs. Br J Anaesth 1998, 81: 193-197.

    Article  CAS  PubMed  Google Scholar 

  13. Brown MA, Zammit VC, Lowe SA: Capillary permeability and extracellular fluid volumes in pregnancy-induced hypertension. Clin Sci (Lond) 1989, 77: 599-604.

    Article  CAS  Google Scholar 

  14. Morita H, Takeuchi K, Funakoshi T, Mizutori M, Maruo T: Potential use of bioelectrical impedance analysis in the assessment of edema in pregnancy. Clin Exp Obstet Gynecol 1999, 26: 151-154.

    CAS  PubMed  Google Scholar 

  15. Vervoort G, Lutterman JA, Smits P, Berden JH, Wetzels JF: Transcapillary escape rate of albumin is increased and related to haemodynamic changes in normo-albuminuric type 1 diabetic patients. J Hypertens 1999, 17: 1911-1916. 10.1097/00004872-199917121-00021

    Article  CAS  PubMed  Google Scholar 

  16. Bedogni G, Polito C, Severi S, Strano CG, Manzieri AM, Alessio M, Iovene A, Battistini N: Altered body water distribution in subjects with juvenile rheumatoid arthritis and its effects on the measurement of water compartments from bioelectric impedance. Eur J Clin Nutr 1996, 50: 335-339.

    CAS  PubMed  Google Scholar 

  17. Pedrinelli R, Dell'Omo G, Bandinelli S, Penno G, Mariani M: Transvascular albumin leakage and forearm vasodilatation to acetylcholine in essential hypertension. Am J Hypertens 2000, 13: 256-261. 10.1016/S0895-7061(00)00250-8

    Article  CAS  PubMed  Google Scholar 

  18. Fleck A, Raines G, Hawker F, Trotter J, Wallace PI, Ledingham IM, Calman KC: Increased vascular permeability: a major cause of hypoalbuminaemia in disease and injury. Lancet 1985, 1: 781-784. 10.1016/S0140-6736(85)91447-3

    Article  CAS  PubMed  Google Scholar 

  19. Christ F, Gamble J, Gartside IB, Kox WJ: Increased microvascular water permeability in patients with septic shock, assessed with venous congestion plethysmography (VCP). Intensive Care Med 1998, 24: 18-27. 10.1007/s001340050509

    Article  CAS  PubMed  Google Scholar 

  20. Marx G, Vangerow B, Burczyk C, Gratz KF, Maassen N, Cobas MM, Leuwer M, Kuse E, Rueckholdt H: Evaluation of noninvasive determinants for capillary leakage syndrome in septic shock patients. Intensive Care Med 2000, 26: 1252-1258. 10.1007/s001340000601

    Article  CAS  PubMed  Google Scholar 

  21. Margarson MP, Soni NC: Effects of albumin supplementation on microvascular permeability in septic patients. J Appl Physiol 2002, 92: 2139-2145. 10.1063/1.1495889

    Article  CAS  PubMed  Google Scholar 

  22. Bethell DB, Gamble J, Pham PL, Nguyen MD, Tran TH, Ha TH, Tran TN, Dong TH, Gartside IB, White NJ, et al.: Noninvasive measurement of microvascular leakage in patients with dengue hemorrhagic fever. Clin Infect Dis 2001, 32: 243-253. 10.1086/318453

    Article  CAS  PubMed  Google Scholar 

  23. Whitney RJ: The measurement of volume changes in human limbs. J Physiol 1953, 121: 1-27.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. Prabhakar U, Eirikis E, Davis HM: Simultaneous quantification of proinflammatory cytokines in human plasma using the LabMAP assay. J Immunol Methods 2002, 260: 207-218. 10.1016/S0022-1759(01)00543-9

    Article  CAS  PubMed  Google Scholar 

  25. Suffredini AF, Hochstein HD, McMahon FG: Dose-related inflammatory effects of intravenous endotoxin in humans: evaluation of a new clinical lot of Escherichia coli O:113 endotoxin. J Infect Dis 1999, 179: 1278-1282. 10.1086/314717

    Article  CAS  PubMed  Google Scholar 

  26. Christ F, Bauer A, Brugger D, Niklas M, Gartside IB, Gamble J: Description and validation of a novel liquid metal-free device for venous congestion plethysmography. J Appl Physiol 2000, 89: 1577-1583.

    CAS  PubMed  Google Scholar 

  27. Gamble J, Gartside IB, Christ F: A reassessment of mercury in silastic strain gauge plethysmography for microvascular permeability assessment in man. J Physiol 1993, 464: 407-422.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  28. Gamble J, Bethell D, Day NP, Loc PP, Phu NH, Gartside IB, Farrar JF, White NJ: Age-related changes in microvascular permeability: a significant factor in the susceptibility of children to shock? Clin Sci (Lond) 2000, 98: 211-216.

    Article  CAS  Google Scholar 

  29. Van Loan MD: Bioelectrical impedance analysis to determine fat-free mass, total body water and body fat. Sports Med 1990, 10: 205-217.

    Article  CAS  PubMed  Google Scholar 

  30. Chiolero RL, Gay LJ, Cotting J, Gurtner C, Schutz Y: Assessment of changes in body water by bioimpedance in acutely ill surgical patients. Intensive Care Med 1992, 18: 322-326.

    Article  CAS  PubMed  Google Scholar 

  31. Mattar JA: Application of total body bioimpedance to the critically ill patient. Brazilian Group for Bioimpedance Study. New Horiz 1996, 4: 493-503.

    CAS  PubMed  Google Scholar 

  32. Roos AN, Westendorp RG, Frolich M, Meinders AE: Weight changes in critically ill patients evaluated by fluid balances and impedance measurements. Crit Care Med 1993, 21: 871-877.

    Article  CAS  PubMed  Google Scholar 

  33. Scheltinga MR, Jacobs DO, Kimbrough TD, Wilmore DW: Identifying body fluid distribution by measuring electrical impedance. J Trauma 1992, 33: 665-670.

    Article  CAS  PubMed  Google Scholar 

  34. Taveira da Silva AM, Kaulbach HC, Chuidian FS, Lambert DR, Suffredini AF, Danner RL: Brief report: shock and multiple-organ dysfunction after self-administration of Salmonella endotoxin. N Engl J Med 1993, 328: 1457-1460. 10.1056/NEJM199305203282005

    Article  CAS  PubMed  Google Scholar 

  35. Suffredini AF, Fromm RE, Parker MM, Brenner M, Kovacs JA, Wesley RA, Parrillo JE: The cardiovascular response of normal humans to the administration of endotoxin. N Engl J Med 1989, 321: 280-287.

    Article  CAS  PubMed  Google Scholar 

  36. Sprong T, Stikkelbroeck N, van der LP, Steeghs L, van Alphen L, Klein N, Netea MG, van der Meer JW, van Deuren M: Contributions of Neisseria meningitidis LPS and non-LPS to proinflammatory cytokine response. J Leukoc Biol 2001, 70: 283-288.

    CAS  PubMed  Google Scholar 

  37. Corriveau CC, Danner RL: Endotoxin as a therapeutic target in septic shock. Infect Agents Dis 1993, 2: 35-43.

    CAS  PubMed  Google Scholar 

  38. Anim-Nyame N, Gamble J, Sooranna SR, Johnson MR, Steer PJ: Microvascular permeability is related to circulating levels of tumour necrosis factor-alpha in pre-eclampsia. Cardiovasc Res 2003, 58: 162-169. 10.1016/S0008-6363(02)00844-1

    Article  CAS  PubMed  Google Scholar 

  39. Girardin E, Grau GE, Dayer JM, Roux-Lombard P, Lambert PH: Tumor necrosis factor and interleukin-1 in the serum of children with severe infectious purpura. N Engl J Med 1988, 319: 397-400.

    Article  CAS  PubMed  Google Scholar 

  40. Oberholzer A, Oberholzer C, Moldawer LL: Cytokine signaling: regulation of the immune response in normal and critically ill states. Crit Care Med 2000, 28: N3-N12. 10.1097/00003246-200004001-00002

    Article  CAS  PubMed  Google Scholar 

  41. Pruitt JH, Welborn MB, Edwards PD, Harward TR, Seeger JW, Martin TD, Smith C, Kenney JA, Wesdorp RI, Meijer S, Cuesta MA, Abouhanze A, et al.: Increased soluble interleukin-1 type ii receptor concentrations in postoperative patients and in patients with sepsis syndrome. Blood 1996, 87: 3282-3288.

    CAS  PubMed  Google Scholar 

  42. Pleiner J, Mittermayer F, Schaller G, MacAllister RJ, Wolzt M: High doses of vitamin c reverse Escherichia coli endotoxin-induced hyporeactivity to acetylcholine in the human forearm. Circulation 2002, 106: 1460-1464. 10.1161/01.CIR.0000030184.70207.FF

    Article  CAS  PubMed  Google Scholar 

  43. Ruot B, Papet I, Bechereau F, Denis P, Buffiere C, Gimonet J, Glomot F, Elyousfi M, Breuille D, Obled C: Increased albumin plasma efflux contributes to hypoalbuminemia only during early phase of sepsis in rats. Am J Physiol Regul Integr Comp Physiol 2003, 284: R707-R713.

    Article  CAS  PubMed  Google Scholar 

  44. O'Dwyer ST, Michie HR, Ziegler TR, Revhaug A, Smith RJ, Wilmore DW: A single dose of endotoxin increases intestinal permeability in healthy humans. Arch Surg 1988, 123: 1459-1464.

    Article  PubMed  Google Scholar 

  45. Suffredini AF, Shelhamer JH, Neumann RD, Brenner M, Baltaro RJ, Parrillo JE: Pulmonary and oxygen transport effects of intravenously administered endotoxin in normal humans. Am Rev Respir Dis 1992, 145: 1398-1403.

    Article  CAS  PubMed  Google Scholar 

  46. Herman DC, Suffredini AF, Parrillo JE, Palestine AG: Ocular permeability after systemic administration of endotoxin in humans. Curr Eye Res 1991, 10: 121-126.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgement

PP is a recipient of a Clinical Fellowship grant of the Netherlands Organisation for Scientific Research (ZonMw).

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Authors and Affiliations

  1. Departments of Intensive Care Medicine and Pharmacology-Toxicology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands

    Lucas TGJ van Eijk & Peter Pickkers

  2. Department of Pharmacology-Toxicology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands

    Paul Smits

  3. Department of Nuclear Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands

    Wim van den Broek

  4. Department of Intensive Care Medicine and Nijmegen UniversityCenter for Infectious Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands

    Martijn PWJM Bouw & Johannes G van der Hoeven

Authors
  1. Lucas TGJ van Eijk
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  2. Peter Pickkers
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  3. Paul Smits
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  4. Wim van den Broek
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  6. Johannes G van der Hoeven
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Correspondence to Peter Pickkers.

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Competing interests

The author(s) declare that they have no competing interests.

Authors' contributions

LTGJvE (medical student) carried out the experiments, performed the statistical analysis and drafted the manuscript. PP conceived the study, and supervised the experiments and writing of the paper. PS participated in the design of the study and corrected the manuscript. WvdB administrated the Alb125 to the participants and measured the plasma radioactivity. MPWJMB (research nurse) assisted with the coordination and practical conduction of the experiments. JGvdH participated in the design of the study and corrected the manuscript. All authors read and approved the final manuscript.

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van Eijk, L.T., Pickkers, P., Smits, P. et al. Microvascular permeability during experimental human endotoxemia: an open intervention study. Crit Care 9, R157 (2005). https://doi.org/10.1186/cc3050

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  • DOI: https://doi.org/10.1186/cc3050

Keywords

  • Septic Patient
  • Total Body Water
  • Bioelectrical Impedance Analysis
  • Forearm Blood Flow
  • Microvascular Permeability

Critical Care

ISSN: 1364-8535