Gene expression patterns in multiple organs in experimentally induced Staphylococcus aureus sepsis in pigs
© Olsen et al.; licensee BioMed Central Ltd. 2013
Published: 5 November 2013
Animal research in sepsis needs analytical tools that can capture and exploit the complexity of the condition. To summarise the disease progression in a porcine model of severe Staphylococcus aureus sepsis, we used principal component analysis (PCA) as a multivariate approach to identify early dynamic expression patterns of 34 selected genes in the liver, lung, and spleen tissue.
Materials and methods
We combined data from two related experimental studies in pigs haematogenously infected with a porcine pathogenic strain of S. aureus [1, 2]. Seventeen infected pigs were euthanised at the following time points post infection (p.i.): 6 hours (n = 3), 12 hours (n = 3), 24 hours (n = 3), 30 hours (n = 1), 36 hours (n = 2), and 48 hours (n = 5). Five healthy controls were managed in parallel. Gene expression of 34 genes related to acute inflammation and haemostasis was measured in the liver, lung, and spleen by quantitative real-time PCR. The data matrix of 22 samples and 102 (34 × 3) variables were log-transformed, scaled to unit variance, and subjected to PCA.
Multivariate analysis (PCA) identified three temporally distinct patterns in gene expression data from the liver, lung, and spleen tissue: pulmonary inflammation was rapidly induced, followed by transient induction of a generalised inflammatory and haemostatic response, and initiation of the hepatic acute-phase response.
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