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Volume 17 Supplement 4

Sepsis 2013

Bacteriological profile and antimicrobial sensitivity pattern of blood culture isolates among septicemia-suspected children at Tikur Anbessa Specialized Hospital and Yekatit 12 Hospital, Addis Ababa, Ethiopia

Background

Septicemia is a systemic disease caused by the spread of microorganisms and their toxins in the blood. These bloodstream infections are a major cause of morbidity and mortality in children in developing country [14]. It has been confirmed by culture that is associated with clinical manifestation and systemic response [57]. It is crucial to continuously monitor any change in the local patterns of infection and susceptibility to various antibiotics. The aim of this study was to determine the bacteriological profile and antimicrobial sensitivity patterns among children suspected of having septicemia.

Materials and methods

A cross-sectional study involved about 201 pediatric patients (≤12 years) was conducted from October 2011 to February 2012 at Tikur Anbessa Specialized Hospital and Yekatit 12 Hospital's pediatric units after the proposal of this study was approved by National Ethics Review Committee. Standard procedure was followed for blood sample collection. Samples were incubated in the BACTEC 9050 System, followed by isolate identifications based on standard microbiological procedures and testing for their susceptibility to antimicrobial agents using the disc diffusion method. Data were analyzed using the SPSS version 19 software package.

Results

Out of 201 study subjects, 110 (54.7%) were male. The majority (147, 73.1%) of them were neonates (≤28 days). The mean length of hospitalization was 11.24 days. Out of the 201 tested blood samples, blood cultures were positive in 56 (27.9%) cases (Figure 1). Gram-negative and Gram-positive bacteria constituted 51.8% and 46.4%, respectively. The most frequent pathogen found was Staphylococcus aureus (23.2%), followed by Serratia marcescens (21.4%), CoNS (19.6%), Klebsiella spp. (16%), Salmonella spp. (5.4%) and Enterobacter cloacae (3.6%) (Figure 2). The majority of bacterial isolates showed high resistance to ampicillin, penicillin, co-trimoxazole, gentamicin and tetracycline. Ciprofloxacin and nalidixic acid were the most effective antimicrobial agents for Gram-negative bacteria, while vancomycin and clindamycin for Gram-positive bacteria (Table 1). Deaths occurred in 25 (12.4%) children, out of which 13 (23.2%) had bacteremia.

Figure 1
figure1

Distribution of 56 blood culture isolates by age interval and gender.

Figure 2
figure2

Distribution of blood culture isolates in children with suspected of having sepsis.

Table 1 Antimicrobial resistance pattern of bacteria isolated from blood culture

Conclusions

The present study revealed that both Gram-positive and Gram-negative bacteria were responsible for bloodstream infections and the majority of the isolates were multidrug resistant. S. aureus and S. marcescens were the most common isolated bacteria from blood cultures. The alarmingly higher percentages of multidrug-resistant isolates urge us to take infection prevention measures and to conduct other large studies for appropriate empiric antibiotic choice.

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Acknowledgements

The authors would like to acknowledge the technical support provided by the members of the Departments of Microbiology and Pediatrics of Tikur Anbessa Specialized and Yekatit 12 Hospitals. They also thank Mr Yoseph Kenea for his excellence statistic support. This work was supported by AHRI/ALERT and AAU.

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Correspondence to Adugna Negussie.

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Negussie, A., Mulugeta, G., Bedru, A. et al. Bacteriological profile and antimicrobial sensitivity pattern of blood culture isolates among septicemia-suspected children at Tikur Anbessa Specialized Hospital and Yekatit 12 Hospital, Addis Ababa, Ethiopia. Crit Care 17, P11 (2013). https://doi.org/10.1186/cc12911

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Keywords

  • Blood Culture
  • Clindamycin
  • Bloodstream Infection
  • Nalidixic Acid
  • Serratia Marcescens