Skip to main content

Volume 16 Supplement 3

Sepsis 2012

  • Poster presentation
  • Open access
  • Published:

Pattern recognition receptors as key players in adrenal gland dysfunction during sepsis

Background

Undergoing systemic inflammation, the innate immune system releases excessive proinflammatory mediators, which finally can lead to organ failure. Pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs) and NOD-like receptors (NLRs), form the interface between bacterial and viral toxins and innate immunity. During sepsis, patients with diagnosed adrenal gland insufficiency are at high risk of developing a multiorgan dysfunction syndrome, which dramatically increases the risk of mortality. To date, little is known about the mechanisms leading to adrenal dysfunction under septic conditions. Here, we investigated the sepsis-related activation of the PRRs, cell inflammation, and apoptosis within adrenal glands.

Methods

Two sepsis models were performed: the polymicrobial sepsis model (caecal ligation and puncture (CLP)) and the LTA-induced intoxication model. All experiments received institutional approval by the Regierungspräsidium Darmstadt. CLP was performed as previously described [1], wherein one-third of the caecum was ligated and punctured with a 20-gauge needle. For LTA-induced systemic inflammation, TLR2 knockout (TLR2-/-) and WT mice were injected intraperitoneally with pure LTA (pLTA; 1 mg/kg) or PBS for 2 hours. To detect potential direct adrenal dysfunction, mice were additionally injected with adrenocorticotropic hormone (ACTH; 100 μg/kg) 1 hour after pLTA or PBS. Adrenals and plasma samples were taken. Gene expressions in the adrenals (rt-PCR), cytokine release (multiplex assay), and the apoptosis rate (TUNEL assay) within the adrenals were determined.

Results

In both models, adrenals showed increased mRNA expression of TLR2 and TLR4, various NLRs, cytokines as well as inflammasome components, NADPH oxidase subunits, and nitric oxide synthases (data not shown). In WT mice, ACTH alone had no effect on inflammation, while pLTA or pLTA/ACTH administration showed increased levels of the cytokines IL-1β, IL-6, and TNFα. TLR2-/- mice indicated no response as expected (Figure 1, left). Interestingly, surviving CLP mice showed no inflammatory adrenal response, whereas nonsurvivors had elevated cytokine levels (Figure 1, right). Additionally, we identified a marked increase in apoptosis of both chromaffin and steroid-producing cells in adrenal glands obtained from mice with sepsis as compared with their controls (Figure 2).

Figure 1
figure 1

Cytokine concentrations of IL-1β, IL-6 and TNFα in plasma and adrenal lysates. From WT and TLR2-/- mice treated with PBS (2 hours), ACTH (100 μg/kg; 1 hour), pLTA (1 mg/kg; 2 hours) or PBS/ACTH and pLTA/ACTH (left) and from WT mice that underwent CLP sham operation (sham), CLP with single (CLP-1P) or double (CLP-2P) puncture (right). Animals that survived after 24-hour CLP are labelled 's' and deceased ones 'ns'. Data are presented as mean ± SEM (n ≥ 6). Statistical significance was determined by one-way ANOVA and Bonferroni's post test (vs. PBS within one group) or t test (WT vs. TLR2-/-). *P < 0.05, **P < 0.01, +P < 0.005, ++P < 0.001, #P < 0.0005, ##P < 0.0001.

Figure 2
figure 2

Apoptosis in murine adrenal glands. From WT and TLR2-/- mice treated with PBS (2 hours), pLTA (1 mg/kg; 2 hours) or pLTA/ACTCH (left) and from WT mice that underwent CLP sham operation (sham), CLP with single (CLP-1P) or double (CLP-2P) puncture (right). TUNEL staining in adrenal sections showing whole adrenals (inserted pictures; 100× magnification), cortex and medulla area (400× magnification). The apoptotic cells appeared with brown nuclear staining.

Conclusion

Taken together, sepsis-induced activation of the PRRs may contribute to adrenal impairment by enhancing tissue inflammation, oxidative stress and culminate in cellular apoptosis, while mortality seems to be associated with adrenal inflammation.

References

  1. Rittirsch D, Huber-Lang MS, Flierl MA, Ward PA: Immunodesign of experimental sepsis by cecal ligation and puncture. Nat Protoc 2009, 4: 31-36.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Cite this article

Tran, T., Kanczkowski, W., Hoesker, V. et al. Pattern recognition receptors as key players in adrenal gland dysfunction during sepsis. Crit Care 16 (Suppl 3), P47 (2012). https://doi.org/10.1186/cc11734

Download citation

  • Published:

  • DOI: https://doi.org/10.1186/cc11734

Keywords