Mechanical ventilation affects local and systemic TNF-alpha and IL-10 in normal swine lung

Introduction

Ventilatory strategies can produce or worsen lung injury and induce a cytokine response in the lung and increase serum cytokine levels [1]. We examined whether ventilatory strategies affect local and systemic inflammatory mediators in a normal swine lung model.

Methods

Five ventilatory strategies were employed in 25 swine animals: group A: low-volume zero PEEP (LVZP): V_T, 10 ml/kg, group B: medium-volume zero PEEP (MVZP): V_T, 20 ml/kg, group C: high-volume zero PEEP (HVZP): VT, 40 ml/kg, group D: low-volume PEEP (LVP): V_T, 10 ml/kg, PEEP, 10 cmH₂O, group E: high-volume PEEP (HVP): V_T 40 ml/kg, PEEP 10 cmH₂O. TNF-a and IL-10 were measured in BAL fluid and serum by ELISA.

Results

Mean cytokine concentration in lung fluid and serum during the experiment are shown in the Table. One animal in LVZP (1 h) and 3 in HVP (2, 3, 2 h) group died before the end of the experiment.

Table

Conclusion

Different injurious ventilatory strategies may induce TNF-a and IL-10 response from alveolar space that may lead to an increase in serum cytokine levels in normal swine lung.