Physiological effects of awake prone position in acute hypoxemic respiratory failure
Critical Care volume 27, Article number: 315 (2023)
The effects of awake prone position on the breathing pattern of hypoxemic patients need to be better understood. We conducted a crossover trial to assess the physiological effects of awake prone position in patients with acute hypoxemic respiratory failure.
Fifteen patients with acute hypoxemic respiratory failure and PaO2/FiO2 < 200 mmHg underwent high-flow nasal oxygen for 1 h in supine position and 2 h in prone position, followed by a final 1-h supine phase. At the end of each study phase, the following parameters were measured: arterial blood gases, inspiratory effort (ΔPES), transpulmonary driving pressure (ΔPL), respiratory rate and esophageal pressure simplified pressure–time product per minute (sPTPES) by esophageal manometry, tidal volume (VT), end-expiratory lung impedance (EELI), lung compliance, airway resistance, time constant, dynamic strain (VT/EELI) and pendelluft extent through electrical impedance tomography.
Compared to supine position, prone position increased PaO2/FiO2 (median [Interquartile range] 104 mmHg [76–129] vs. 74 [69–93], p < 0.001), reduced respiratory rate (24 breaths/min [22–26] vs. 27 [26–30], p = 0.05) and increased ΔPES (12 cmH2O [11–13] vs. 9 [8–12], p = 0.04) with similar sPTPES (131 [75–154] cmH2O s min−1 vs. 105 [81–129], p > 0.99) and ΔPL (9 [7–11] cmH2O vs. 8 [5–9], p = 0.17). Airway resistance and time constant were higher in prone vs. supine position (9 cmH2O s arbitrary units−3 [4–11] vs. 6 [4–9], p = 0.05; 0.53 s [0.32–61] vs. 0.40 [0.37–0.44], p = 0.03). Prone position increased EELI (3887 arbitrary units [3414–8547] vs. 1456 [959–2420], p = 0.002) and promoted VT distribution towards dorsal lung regions without affecting VT size and lung compliance: this generated lower dynamic strain (0.21 [0.16–0.24] vs. 0.38 [0.30–0.49], p = 0.004). The magnitude of pendelluft phenomenon was not different between study phases (55% [7–57] of VT in prone vs. 31% [14–55] in supine position, p > 0.99).
Prone position improves oxygenation, increases EELI and promotes VT distribution towards dependent lung regions without affecting VT size, ΔPL, lung compliance and pendelluft magnitude. Prone position reduces respiratory rate and increases ΔPES because of positional increases in airway resistance and prolonged expiratory time. Because high ΔPES is the main mechanistic determinant of self-inflicted lung injury, caution may be needed in using awake prone position in patients exhibiting intense ΔPES.
Clinical trail registeration: The study was registered on clinicaltrials.gov (NCT03095300) on March 29, 2017.
In intubated patients with moderate-to-severe acute respiratory distress syndrome, prone positioning reduces intrapulmonary shunt and generates lung recruitment, optimizes ventilation/perfusion matching, lowers alveolar dead space and reduces right ventricle afterload . Through all these mechanisms, prone positioning improves gas exchange and attenuates ventilator-induced lung injury . Robust evidence supports the systematic use of prone positioning to improve survival among intubated patients with moderate-to-severe acute respiratory distress syndrome [3, 4].
In recent years, prone position has been proposed in non-intubated patients with acute hypoxemic respiratory failure, with positive effects on arterial oxygenation [5,6,7,8,9,10]. A large randomized meta-trial and one observational study demonstrated that awake prone positioning in patients with hypoxemia due to COVID-19 reduces the need for endotracheal intubation and, possibly, mortality [11, 12]. However, other data did not confirm these findings and highlighted the possible risks related to intubation delays due to only transient oxygenation improvement produced by prone position [13, 14].
Despite plenty of clinical data obtained during the COVID-19 pandemic, few available studies address the physiological effects of prone position in spontaneously breathing humans with acute hypoxemic respiratory failure [15,16,17].
We conducted a sequential, crossover trial to comprehensively evaluate the effects of awake prone positioning on gas exchange, effort-to-breathe, lung volumes and inflation pattern in adult patients with moderate-to-severe acute hypoxemic respiratory failure undergoing high-flow nasal oxygen.
This sequential crossover study was conducted in the intensive care unit of a university hospital in Italy between October 2018 and June 2020. The study was funded by an unrestricted research grant by the European Society of Intensive Care Medicine (ESICM-2017 Bernhard Dräger Award). The study was approved by institutional review board (ID 1506-ethics committee Fondazione Policlinico A. Gemelli IRCCS, Rome Italy) and was conducted in accordance with the declaration of Helsinki. All enrolled patients provided written informed consent to participating in the study and data analysis. The study protocol was registered on clinicaltrials.gov (NCT03095300) on March 29, 2017.
Adult patients admitted to the intensive care unit due to acute hypoxemic respiratory failure were assessed for the enrolment. Acute hypoxemic respiratory failure was defined as an acute onset syndrome characterized by new or worsening impairment in oxygenation. Patients were considered eligible for inclusion if the following criteria were met: PaO2/FiO2 < 200, measured in the supine position while the patients was breathing heated and humidified high-flow oxygen through a non-rebreather face mask (60 L/min, temperature of the humidification chamber set at 37 °C, FiO2 set to achieve a SpO2 > 92% and < 98%—because of the high flows, nominal FiO2 was considered a reliable estimate of the actual one); PaCO2 < 45 mmHg; no of history of chronic respiratory failure or moderate-to-severe cardiac failure (New York Hear Association grade > II or left ventricular ejection fraction < 50%); body mass index < 30 kg/m2; absence of any contraindication to prone positioning (detailed in the Additional file 2: Supplementary material).
Exclusion criteria were: more than 48 h from the admission in the intensive care unit; acute exacerbation of asthma or chronic obstructive pulmonary disease; chest trauma; cardiogenic pulmonary edema; severe neutropenia (< 500 white blood cell count/mm3); hemodynamic instability (systolic blood pressure < 90 mmHg or mean arterial pressure < 65 mmHg) and/or lactic acidosis (serum lactate > 5 mmol/L) and/or clinically diagnosed shock; metabolic acidosis (pH < 7.30); chronic kidney failure requiring dialysis before intensive care unit admission; altered consciousness, defined by a Glasgow coma scale < 13; vomiting and/or upper gastrointestinal bleeding.
Patients received high-flow nasal oxygen for 1 h in the supine semirecumbent position. Gas flow was set at 60 L/min, the temperature of the humidification chamber (MR860 or ARIVO2, Fisher and Paykel healthcare) was set according to patient's comfort, FiO2 was titrated to maintain SpO2 > 92% and < 98%. After 1 h of high-flow treatment in the supine semirecumbent position, patients were placed in the prone position for 2 h and then placed again in the supine semirecumbent position to undergo a final hour of high-flow nasal oxygen.
For safety reasons, enteral feeding was interrupted 1 h before prone positioning and re-established after the study ended.
Patient’s demographics and main clinical characteristics were collected at study entry. During the study, each patient underwent standard monitoring including 5-lead electrocardiogram, invasive blood pressure and pulse oximetry. A polyfunctional nasogastric tube provided with an esophageal balloon (Nutrivent, Sidam, Italy) was placed and secured at a depth of 38–42 cm to measure esophageal pressure (PES). The esophageal balloon was filled with 4 ml of air, which has been shown to be a non-stress volume providing reliability in a wide pressure range for the Nutrivent catheter . To ensure intra-individual reproducibility, the esophageal balloon was deflated and, after checking adequate zeroing, re-inflated before all measurements. A pressure transducer measured PES (FluxMed v. 1802, MBMED, Buenos Aires, Argentina). An electrical impedance tomography (EIT) belt (LuMon, Sentec, Switzerland) with 16 electrodes was placed around the thorax between the fifth or sixth parasternal intercostal space and connected to a dedicated device to record electrical impedance signals. EIT data were acquired at a frame rate of 40 Hz. A detailed description of EIT signal processing procedure used in this study is provided elsewhere  and in Additional file 2: Supplementary material.
At the end of each step, the following data were collected: respiratory rate, SpO2, blood gases, heart rate, arterial pressure, dyspnea and discomfort as defined by a visual analogic scales (VAS) [19,20,21] (Additional file 1: E-Figures 1–2). EIT and PES were recorded for 15 min at the end of each study phase, once a stable breathing pattern was obtained.
PES and EIT signals were acquired in phase, amplified, low-pass filtered, digitalized at 40 Hz and stored in a personal computer (FluxMed v. 1802, MBMED, Buenos Aires, Argentina). All breaths from 15-min recordings were analyzed with MATLAB (Mathworks, Portola Valley, CA, USA). Results from all breaths in the 15-min recording were averaged for each study step.
The primary objectives of this study were to assess the effects of prone position on arterial oxygenation (as defined by the PaO2/FiO2 ratio) and to establish the proportion of patients who underwent the procedure without displaying procedure-related serious adverse events, defined as any of the following: oxygen desaturations (SpO2 < 90%), hemodynamic instability (systolic arterial pressure < 80 mmHg or heart rate > 120 beats per minute), or displacement of central venous or arterial line.
Secondary endpoints of the study were the effects of prone position on:
Breathing pattern: respiratory rate, inspiratory effort (the negative deflection of PES tracing during inspiration), PES simplified pressure time product (the chest wall recoil was neglected in all phases due to the impossibility of performing occlusions in non-intubated patients) [22, 23], VAS discomfort and dyspnea.
Gas exchange: PaCO2 and corrected minute ventilation. Minute ventilation was expressed in arbitrary units and derived from the EIT signal: corrected minute ventilation was calculated as minute ventilation multiplied by the ratio of patient’s PaCO2 to 40 mmHg (with lower values indicating improved CO2 clearance, reduced CO2 production, or both) .
Respiratory mechanics: The end-expiratory PES, which reflects the superimposed pressure on dorsal lung zones , end-expiratory transpulmonary pressure (PL, calculated as airway pressure-PES), quasi-static transpulmonary driving pressure (defined as the difference between end-inspiratory PL and end-expiratory PL), airway resistance (Additional file 2: Supplementary material 3).
Lung inflation pattern, measured with the EIT globally and regionally in the four regions of interests (ROI: ventral, mid-ventral, mid-dorsal, dorsal-Additional file 1: E-Figure 3—for the exact number of rows in each ROI refer to Additional file 2: Supplementary material 2). For this purpose, ROIs were defined from a standardized lung contour per patient and are specific for the impedance software used .
Analyzed EIT outcomes were: global and regional tidal volume, expressed in arbitrary units and calculated on a pixel-by-pixel basis; global and regional lung compliance, calculated as the ratio of tidal volume to quasi-static transpulmonary driving pressure; amount of pendelluft, expressed in terms of % of tidal volume (Additional file 2: Supplementary material 4) [19, 26,27,28]; end-expiratory lung impedance (EELI), expressed in arbitrary units and derived from the impedance signal and the lung strain definition [29,30,31], as described elsewhere ; global EELI and regional EELI distribution in the four ROIs; dynamic lung strain, computed as the ratio of tidal volume to the functional residual capacity: for this purpose, functional residual capacity was approximated to EELI; regional dynamic strain, computed as above, in the four ROIs; the amount of overstretched lung regions, defined as the percentage of lung pixels exhibiting dynamic strain greater than two ; inspiratory and expiratory times were assessed according to the time of zero flow (first derivative of the EIT signal), airway resistance calculated from the expiratory time constant as detailed in Additional file 2: Supplementary material and Additional file 1: E-Figure 4.
Respiratory mechanics and lung inflation pattern were analyzed according to a methodology described elsewhere  and in Additional file 2: Supplementary material. Consistently with a previous investigations, airway pressure during high-flow nasal oxygen was assumed to be constant and equal to 2.5 cmH2O [23, 24, 32]. For all calculations, beginning of inspiration and expiration was defined on the EIT vs. time tracing, when its first derivative became positive (end of expiration) and negative (end of inspiration).
Sample size calculation
Given the physiological design of the study, we did not perform a formal sample size calculation. Consistently with previous investigations with similar design on the topic [19, 23, 24, 33, 34], we planned to enroll 15 patients, which is an adequate sample to draw significant conclusions on these specific endpoints.
Categorical data are expressed as the event rate (%), while continuous data are expressed as the median [interquartile range]. Normality in the distribution of continuous variables was assessed with the Kolmogorov–Smirnov test.
Normally distributed quantitative variables in the three study steps were compared using ANOVA for repeated measures, with Bonferroni’s correction added for paired comparisons. Ordinal and non-normally distributed quantitative variables were analyzed using the Friedman test, with post hoc Dunn's test to adjust for multiple comparisons during pairwise testing of study phases. P-values, mean differences and confidence intervals for paired comparisons are displayed, and results with two-tail p ≤ 0.05 were considered statistically significant. For p-values greater than 0.01, two digits are provided and rounded to the closest second digit.
Correlations between continuous variables were assessed with Pearson’s correlation, and the r and p values are reported.
Statistical analysis was performed with SPSS 26.0, MATLAB R2021, and GraphPad Prism V 9.00.
The demographics and clinical characteristics of the enrolled patients are shown in Table 1. The median [interquartile range] PaO2/FiO2 at enrolment was 116 mmHg [97–127].
Gas exchange and subjective symptoms
None of the fifteen enrolled patients experienced any serious adverse events. Thirteen out of 15 patients showed increased blood oxygenation after 2 h of prone positioning (mean difference 45 mmHg [95% CI 23–68], p = 0.002). However, upon re-supination a decrease in oxygenation was observed compared with prone positioning (mean difference − 67 mmHg [95% CI 94–41], p < 0.001) and supine position before pronation (mean difference − 22 mmHg [95% CI − 12 to − 32], p = 0.001) (Fig. 2).
PaCO2 did not change during any of the positional changes (p = 0.47) (Fig. 2).
There was no change in perceived dyspnea among the three study phases, but patients exhibited less tolerability for the prone position when resupinated (p = 0.01) (Fig. 2).
Effort to breath and respiratory mechanics
Respiratory rate decreased during prone positioning (mean difference − 2 breaths per minute [95% CI − 6 to − 1], p = 0.05), a benefit not maintained after re-supination (Fig. 2).
Compared to supine position, prone position increased inspiratory effort (ΔPES mean difference 2 cmH2O [95% CI 1–4], p = 0.04) (Fig. 3). During prone position, the increase in ΔPES was not accompanied by changes in ΔPL (p = 0.63).
Prone position was associated with changes in respiratory system resistive properties, with an increased time constant compared to both supine phases (p = 0.03, p = 0.05). This was caused by increased airway resistance (p = 0.05) with prolonged expiratory time (p = 0.05) during prone position.
Prone-induced changes in airway resistance were related to the change in ΔPES (r = 0.53, p = 0.04) (Additional file 1: E-Figure 5).
Simplified minute PTPES did not differ between any of the study phases (p > 0.05 for all comparisons) (Fig. 3).
In prone position, end-expiratory esophageal pressure was lower (p < 0.001), and end-expiratory transpulmonary pressure was higher (p = 0.03) than during supine position (Fig. 3).
Compared to supine position, prone position did not yield changes in tidal volume (all p > 0.05) (Fig. 4). Prone position altered tidal volume distribution, resulting in a significant increase in ventilation of the ventral ROI (mean difference 20% [95% CI 3–37], p = 0.01) and dorso-dorsal ROI (mean difference 35% [95% CI − 8 to 78], p = 0.05) compared to supine position (Fig. 5).
Given the unchanged ΔPL and tidal volume, no changes in lung global and regional compliance were detected (p > 0.05 for all comparisons).
Pendelluft was common in our cohort (31% [14–55] of tidal volume during supine position, 55% [7–57] during prone position, 44% [13–66] after re-supination), without significant differences between treatments (p > 0.05 for all comparisons).
End-expiratory lung impedance
Prone position increased EELI compared to supine phases before and after the intervention (mean % increase 279% [95%CI: 133 to 330], p = 0.002 and 106% [95%CI: 22 to 448] arbitrary units, p = 0.01, respectively) (Fig. 4, Table 2).
The increase in EELI occurred throughout all lung regions, but was prominent in dorsal ROIs.
The increase in EELI led to a reduction in the dynamic strain during prone position, compared to both supine positions before and after pronation (mean difference − 0.22 [95% CI − 0.33 to − 0.11], p = 0.004; − 0.17 [95% CI − 0.30 to − 0.04], p = 0.04, respectively). No difference was observed in dynamic strain between the two supine phases (p > 0.99). Dynamic strain was predominantly ventral in supine position and predominantly dorsal in prone position (Table 2).
The results of this sequential study on the physiological effects of awake prone positioning in patients with moderate-to-severe hypoxemic respiratory failure undergoing high-flow nasal oxygen can be summarized as follows:
Awake prone positioning improves arterial oxygenation without serious periprocedural adverse events. However, the benefit on oxygenation is transient and, after supination, oxygenation may significantly worsen, likely reflecting patient’s deterioration.
Awake prone positioning reduces respiratory rate but increases ΔPES, with no effects on ΔPL, VT, quasi-static lung compliance, simplified minute PTPES, PaCO2 and dyspnea. These effects are mediated by increased airway resistance to flow throughout all the respiratory cycle, with higher expiratory time constant and prolonged expiration.
Awake prone positioning increases EELI. This occurs due to recruitment of dorsal lung regions, enhanced expiratory pressure produced by higher resistance to expiratory flow and higher end-expiratory transpulmonary pressure.
In prone position, the increase in EELI combined to a shift of the VT towards dorsal lung regions causes a reduction in global and regional lung strain, reflecting more homogenous lung inflation.
Prone positioning does not affect the magnitude of the pendelluft phenomenon.
In intubated patients with moderate-to-severe ARDS, prone position improves oxygenation, limits ventilator-induced lung injury and decreases mortality [1, 3, 4]. During the COVID-19 pandemic, awake prone position has been extensively applied, with favorable effects on arterial oxygenation . Subsequent randomized studies confirmed the beneficial effects on oxygenation, but showed conflicting results on the capability of awake prone position to reduce the rate of endotracheal intubation and improve patient-centered outcomes [12, 14].
Over the past decade, the management of patients with hypoxemic respiratory failure has changed significantly. High-flow nasal oxygen has emerged as a means of avoiding endotracheal intubation and minimizing the adverse effects of sedation and invasive mechanical ventilation [35, 36]. However, intubation after noninvasive support is still required in many cases, and failure of noninvasive support results in increased mortality [37,38,39]. A better understanding of the physiology of spontaneous breathing has given rise to a new clinical challenge: the careful balance between using noninvasive devices to avoid intubation vs. the risk of exposure to delayed intubation combined with harmful spontaneous breathing leading to patient self-inflicted lung injury (SILI). SILI arises from increased ΔPES and lung inhomogeneity, which cause uneven lung inflation and local overstretch, especially in the dependent lung . This partially differs from ventilator-induced lung injury caused by controlled mechanical ventilation, which mostly occurs in the non-dependent lung exposed to mechanical stress and strain imposed in the absence of spontaneous breathing .
The major SILI determinant is the intensity of ΔPES : accordingly, ΔPES and its changes as a response to treatment represent the most relevant determinants of the subsequent need for intubation during noninvasive support . This indicates that any noninvasive intervention should be interpreted addressing its effect both on oxygenation and ΔPES, with ΔPES reflecting the risk of SILI .
Several studies showed that awake prone position improves oxygenation, but few studies elucidated the effects of awake prone positioning on ΔPES. In an animal lung injury model, prone position was shown to reduce ΔPES and minimize SILI . However, subsequent studies in non-intubated humans with acute hypoxemic respiratory failure did not confirm these findings, and showed that prone position mainly reduces respiratory rate, with unchanged, or even increased, ΔPES [16, 17]. This discrepancy between animals and humans may be explained by the specific features of experimental lung injury and the animals studied, whose posture is naturally prone.
Our study confirms that prone position can improve oxygenation without serious adverse events related to the procedure. This is attributable to the observed increase in EELI, caused by the positional change and, likely, to enhanced positive expiratory pressure produced by increased airway resistance to expiratory flow and higher transpulmonary end-expiratory pressure . Prone-induced increases in functional residual capacity have been documented in non-intubated humans since 1960s  and are consistent with the mechanism of action of prone position in intubated patients with acute respiratory distress syndrome . In patients with acute hypoxemic respiratory failure, improvement in oxygenation may help avoid endotracheal intubation, since hypoxemia is a relevant cause of treatment failure during noninvasive support . However, in our study, oxygenation significantly worsened (even compared to study start) when the patient was re-placed in supine position. Other investigators have reported this phenomenon [13, 14], but the exact mechanism behind this is unknown.
In our cohort, prone positioning reduced respiratory rate but increased ΔPES. The reduction in respiratory rate is a well-documented effect of prone position in spontaneously breathing patients [15,16,17]. Higher ΔPES in the prone position has been previously observed . Our results indicate that the increase in ΔPES in prone position is induced by increased airway resistance to flow, with unchanged VT, lung compliance and quasi-static transpulmonary driving pressure. ΔPES includes the resistive and elastic workload per breath: in our study, prone position increased the resistive workload, with unchanged elastic workload. Increased expiratory resistance (i.e., limited expiratory flow) in prone vs. sitting position has been shown in healthy individuals [49, 50] and may occur due to small airway closure and gravitational changes in lower airways . Moreover, prone position necessitates breathing with the head in a laterally rotated position, which causes dealignment of the cranio-pharyngeal axis: this can increase resistance due to geometrical changes in the shape of upper airways and a heightened risk of turbulent flow [52, 53]. All these mechanisms explain the prone-induced increase in ΔPES (higher respiratory resistance) and reduction in respiratory rate (longer expiratory time constant due to higher expiratory resistance).
In our study, we neglected the amount of ΔPES needed to overcome chest wall recoil pressure, as we were unable to perform occlusions. This is particularly relevant during the prone position due to an increase in chest wall elastance . As a result, we may have underestimated the ΔPES during this phase more than in supine position, and the actual increase in ΔPES during prone position is likely higher than we were able to demonstrate.
In our study, prone position reduced global and regional dynamic strain, which represents the most relevant determinant of ventilator-induced lung injury during controlled ventilation in patients with acute respiratory distress syndrome. This may contribute to the beneficial effects of prone position observed in spontaneously breathing subjects in clinical and observational studies [12, 45, 55].
In our study, prone position did not affect the pendelluft phenomenon, which is one of the mechanisms of SILI [40, 42]. The primary determinant of the pendelluft phenomenon is ΔPES [19, 40, 42], that was not reduced, and even increased, with prone position.
Our results have relevant clinical implications:
Prone-induced improvement in oxygenation may help avoid endotracheal intubation, since hypoxemia is a relevant cause of treatment failure during noninvasive support . However, oxygenation significantly worsened (even compared to study start) when the patient is placed again in supine position. This may yield intubation delays, with possible detrimental effects on clinical outcome. This may also explain the dose–response relationship between the intervention and clinical outcome observed in a clinical trial , where a higher duration of prone sessions led to reduced risk of subsequent endotracheal intubation.
Prone position homogenizes lung inflation, reduces global and regional dynamic strain and respiratory rate, does not affect the amplitude of the pendelluft phenomenon, VT, lung compliance and quasi-static driving pressure, and increases ΔPES. Although dynamic strain is the main determinant of ventilator-induced lung injury, ΔPES and pendelluft are major determinants of SILI [12, 56]. Prone position appears to mitigate the risk of lung injury due to dynamic strain, but not the injury due to ΔPES and pendelluft. Also, high ΔPES may yield diaphragm injury and muscle exhaustion, which may cause treatment failure and are associated with worse long-term clinical outcome [57, 58]. Interestingly, in a previous study, the entity of ΔPES-increase due to prone position was associated with the subsequent need for intubation . Although the absolute increase in ΔPES was limited in our cohort, our results may indicate that awake prone positioning may not be indicated for all patients with acute hypoxemic respiratory failure, but only for those exhibiting low-to-normal ΔPES. Those with high ΔPES are rather likely to benefit from an approach specifically aimed at reducing the inspiratory effort: this may explain why noninvasive ventilation (that best reduces ΔPES) may result in reduced rate of intubation vs. high-flow nasal oxygen combined with prone position among patients with high ΔPES [19, 59,60,61]. Discrepancy between the huge clinical benefit by prone positioning in intubated patients vs. the milder observed in non-intubated patients may be related to the inability of the intervention to modulate ΔPES.
Our study has limitations. First, we assessed the effects of prone positioning after 2 h, while it has been shown that the most clinical benefit by prone positioning is observed in patients who remain prone for longer periods ; however, our design is consistent with that of studies addressing the physiological effects of prone positions in intubated patients . Second, because of the impossibility to perform occlusions, we neglected the respiratory workload related to chest-wall recoil pressure: since prone position increases chest wall elastance, this should not alter, and could even strengthen, our results on ΔPES. Third, we assumed that inspiratory and expiratory resistance were equal, while they can be different: assessing resistance in non-intubated patients is complex, and we deem this may be an acceptable approximation. Fourth, prone-induced increases in airway resistance may have affected end-expiratory pressure, which was assumed to be constant in our study: although this might have slightly changes our dynamic strain calculations, we believe this does not affect the overall meaning of the investigation. Fifth, our physiological measurements were obtained with electrical impedance tomography, which provides data in arbitrary units rather than in volume/flow; however, the electrical impedance tomography signal is strictly linked with changes in lung aeration , and the crossover design of our study allows to detect the changes induced by the intervention, independently from the absolute values of flow and volume in the study phases. Sixth, absolute values of EELI rely on the physiological relationship between stress and strain; therefore, absolute EELI and dynamic strain values should be interpreted cautiously. Finally, we did not measure gastric pressure, making impossible to establish whether expiratory muscles recruitment induced by body position may have contributed to the observed results.
In patients undergoing high-flow nasal oxygen and exhibiting moderate-to-severe hypoxemia due to acute respiratory failure, prone position improves oxygenation by enhancing recruitment of dorsal lung regions, homogenizes ventilation distribution and reduces respiratory rate. Prone position does not affect VT, the amount of pendelluft, transpulmonary driving pressure and lung compliance, but increases ΔPES because of higher airway resistance with longer expiratory time constant. Because ΔPES and pendelluft are the main mechanistic determinants of SILI, caution may be needed in the use of prone position in patients exhibiting high ΔPES.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Acute respiratory distress syndrome
Positive end-expiratory pressure
- ΔP L :
Transpulmonary driving pressure
- ΔP ES :
- sPTPES :
Simplified esophageal pressure pressure–time product
- V T :
End-expiratory lung impedance
Region of interest
Gattinoni L, Carlesso E, Taccone P, Polli F, Guérin C, Mancebo J. Prone positioning improves survival in severe ARDS: a pathophysiologic review and individual patient meta-analysis. Miner Anestesiol. 2010;76:448–54.
Guerin C, Baboi L, Richard JC. Mechanisms of the effects of prone positioning in acute respiratory distress syndrome. Intensive Care Med. 2014;40:1634–42.
Guérin C, Reignier J, Richard J-C, Beuret P, Gacouin A, Boulain T, et al. Prone positioning in severe acute respiratory distress syndrome. N Engl J Med. 2013;368:2159–68.
Fan E, Del Sorbo L, Goligher EC, Hodgson CL, Munshi L, Walkey AJ, et al. An Official American Thoracic Society/European Society of Intensive Care Medicine/Society of Critical Care Medicine Clinical Practice Guideline: mechanical ventilation in adult patients with acute respiratory distress syndrome. Am J Respir Crit Care Med. 2017;195:1253–63.
Coppo A, Bellani G, Winterton D, Di Pierro M, Soria A, Faverio P, et al. Feasibility and physiological effects of prone positioning in non-intubated patients with acute respiratory failure due to COVID-19 (PRON-COVID): a prospective cohort study. Lancet Respir Med. 2020;8:765–74.
Thompson AE, Ranard BL, Wei Y, Jelic S. Prone positioning in awake, nonintubated patients with COVID-19 hypoxemic respiratory failure. JAMA Intern Med. 2020;180:1537–9.
Elharrar X, Trigui Y, Dols A-M, Touchon F, Martinez S, Prud’homme E, et al. Use of prone positioning in nonintubated patients with COVID-19 and hypoxemic acute respiratory failure. JAMA. 2020;323:2336–8.
Menga LS, Berardi C, Ruggiero E, Grieco DL, Antonelli M. Noninvasive respiratory support for acute respiratory failure due to COVID-19. Curr Opin Crit Care. 2022;28:25–50.
Stilma W, Åkerman E, Artigas A, Bentley A, Bos LD, Bosman TJC, et al. Awake proning as an adjunctive therapy for refractory hypoxemia in non-intubated patients with COVID-19 acute respiratory failure: guidance from an international group of healthcare workers. Am J Trop Med Hyg. 2021;104:1676–86.
Scaravilli V, Grasselli G, Castagna L, Zanella A, Isgrò S, Lucchini A, et al. Prone positioning improves oxygenation in spontaneously breathing nonintubated patients with hypoxemic acute respiratory failure: A retrospective study. J Crit Care. 2015;30:1390–4. https://doi.org/10.1016/j.jcrc.2015.07.008.
Perez-Nieto OR, Escarraman-Martinez D, Guerrero-Gutierrez MA, Zamarron-Lopez EI, Mancilla-Galindo J, Kammar-García A, et al. Awake prone positioning and oxygen therapy in patients with COVID-19: the APRONOX study. Eur Respir J. 2022. https://doi.org/10.1183/13993003.00265-2021.
Ehrmann S, Li J, Ibarra-Estrada M, Perez Y, Pavlov I, McNicholas B, et al. Awake prone positioning for COVID-19 acute hypoxaemic respiratory failure: a randomised, controlled, multinational, open-label meta-trial. Lancet Respir Med. 2021;9:1387–95.
Ferrando C, Suarez-Sipmann F, Mellado-Artigas R, Hernández M, Gea A, Arruti E, et al. Clinical features, ventilatory management, and outcome of ARDS caused by COVID-19 are similar to other causes of ARDS. Intensive Care Med. 2020.
Alhazzani W, Parhar KKS, Weatherald J, Al Duhailib Z, Alshahrani M, Al-Fares A, et al. Effect of awake prone positioning on endotracheal intubation in patients with COVID-19 and acute respiratory failure: a randomized clinical trial. JAMA. 2022;327:2104–13.
Lehingue S, Allardet-Servent J, Ferdani A, Hraeich S, Forel J-M, Arnal J-M, et al. Physiologic effects of the awake prone position combined with high-flow nasal oxygen on gas exchange and work of breathing in patients with severe COVID-19 pneumonia: a randomized crossover trial. Crit care Explor. 2022;4:e0805.
Chiumello D, Chiodaroli E, Coppola S, Borlino SC, Granata C, Pitimada M, et al. Awake prone position reduces work of breathing in patients with COVID-19 ARDS supported by CPAP. Ann Intensive Care. 2021. https://doi.org/10.1186/s13613-021-00967-6.
Cammarota G, Rossi E, Vitali L, Simonte R, Sannipoli T, Anniciello F, et al. Effect of awake prone position on diaphragmatic thickening fraction in patients assisted by noninvasive ventilation for hypoxemic acute respiratory failure related to novel coronavirus disease. Crit Care BioMed Central. 2021;25:1–10. https://doi.org/10.1186/s13054-021-03735-x.
Mojoli F, Iotti GA, Torriglia F, Pozzi M, Volta CA, Bianzina S, et al. In vivo calibration of esophageal pressure in the mechanically ventilated patient makes measurements reliable. Crit Care. 2016;20:98.
Menga LS, Delle Cese L, Rosà T, Cesarano M, Scarascia R, Michi T, et al. Respective effects of helmet pressure support, continuous positive airway pressure, and nasal high-flow in hypoxemic respiratory failure: a randomized crossover clinical trial. Am J Respir Crit Care Med. 2023;207:1310–23.
Dres M, Similowski T, Goligher EC, Pham T, Sergenyuk L, Telias I, et al. Dyspnoea and respiratory muscle ultrasound to predict extubation failure. Eur Respir J. 2021;58:2100002.
Menga LS, Grieco DL, Rosà T, Cesarano M, Cese LD, Berardi C, et al. Dyspnoea and clinical outcome in critically ill patients receiving noninvasive support for covid-19 respiratory failure: Post hoc analysis of a randomised clinical trial. ERJ Open Res. 2021;7:3–6.
Bongiovanni F, Grieco DL, Anzellotti GM, Menga LS, Michi T, Cesarano M, et al. Gas conditioning during helmet noninvasive ventilation: effect on comfort, gas exchange, inspiratory effort, transpulmonary pressure and patient-ventilator interaction. Ann Intensive Care. 2021;11:184. https://doi.org/10.1186/s13613-021-00972-9.
Grieco DL, Menga LS, Raggi V, Bongiovanni F, Anzellotti GM, Tanzarella ES, et al. Physiological comparison of high-flow nasal cannula and helmet noninvasive ventilation in acute hypoxemic respiratory failure. Am J Respir Crit Care Med. 2020;201:303–12.
Mauri T, Turrini C, Eronia N, Grasselli G, Volta CA, Bellani G, et al. Physiologic effects of high-flow nasal cannula in acute hypoxemic respiratory failure. Am J Respir Crit Care Med. 2017;195:1207–15.
Yoshida T, Amato MBP, Grieco DL, Chen L, Lima CAS, Roldan R, et al. Esophageal manometry and regional transpulmonary pressure in lung injury. Am J Respir Crit Care Med. 2018;197:1018–26.
Yoshida T, Torsani V, Gomes S, De Santis RR, Beraldo MA, EL Costa V, et al. Spontaneous effort causes occult pendelluft during mechanical ventilation. Am J Respir Crit Care Med. 2013;188:1420–7.
Chi Y, Zhao Z, Frerichs I, Long Y, He H. Prevalence and prognosis of respiratory pendelluft phenomenon in mechanically ventilated ICU patients with acute respiratory failure: a retrospective cohort study. Ann Intensive Care. 2022. https://doi.org/10.1186/s13613-022-00995-w.
Otis AB, McKerrow CB, Bartlett RA, Mead J, McIlroy MB, Selver-Stone NJ, et al. Mechanical factors in distribution of pulmonary ventilation. J Appl Physiol. 1956;8:427–43.
Chiumello D, Carlesso E, Cadringher P, Caironi P, Valenza F, Polli F, et al. Lung stress and strain during mechanical ventilation for acute respiratory distress syndrome. Am J Respir Crit Care Med. 2008;178:346–55.
Protti A, Cressoni M, Santini A, Langer T, Mietto C, Febres D, et al. Lung stress and strain during mechanical ventilation: any safe threshold? Am J Respir Crit Care Med. 2011;183:1354–62.
Protti A, Andreis DT, Monti M, Santini A, Sparacino CC, Langer T, et al. Lung stress and strain during mechanical ventilation: any difference between statics and dynamics? Crit Care Med. 2013;41:1046–55.
Mauri T, Alban L, Turrini C, Cambiaghi B, Carlesso E, Taccone P, et al. Optimum support by high-flow nasal cannula in acute hypoxemic respiratory failure: effects of increasing flow rates. Intensive Care Med. 2017;43:1453–63.
L’Her E, Deye N, Lellouche F, Taille S, Demoule A, Fraticelli A, et al. Physiologic effects of noninvasive ventilation during acute lung injury. Am J Respir Crit Care Med. 2005;172:1112–8.
Natalini D, Grieco DL, Santantonio MT, Mincione L, Toni F, Anzellotti GM, et al. Physiological effects of high-flow oxygen in tracheostomized patients. Ann Intensive Care. 2019;9:114. https://doi.org/10.1186/s13613-019-0591-y.
Maggiore SM, Grieco DL, Lemiale V. The use of high-flow nasal oxygen. Intensive Care Med. 2023;33:65–72. https://doi.org/10.1007/s00134-023-07067-y.
Rochwerg B, Einav S, Chaudhuri D, Mancebo J, Mauri T, Helviz Y, et al. The role for high flow nasal cannula as a respiratory support strategy in adults: a clinical practice guideline. Intensive Care Med. 2020;46:2226–37.
Bellani G, Laffey JG, Pham T, Madotto F, Fan E, Brochard L, et al. Noninvasive ventilation of patients with acute respiratory distress syndrome. Insights from the LUNG SAFE study. Am J Respir Crit Care Med. 2017;195:67–77. https://doi.org/10.1164/rccm.201606-1306OC.
Demoule A, Girou E, Richard J-C, Taille S, Brochard L. Benefits and risks of success or failure of noninvasive ventilation. Intensive Care Med. 2006;32:1756–65.
Kang BJ, Koh Y, Lim C-M, Huh JW, Baek S, Han M, et al. Failure of high-flow nasal cannula therapy may delay intubation and increase mortality. Intensive Care Med. 2015;41:623–32. https://doi.org/10.1007/s00134-015-3693-5.
Yoshida T, Fujino Y, Amato MBP, Kavanagh BP. Fifty years of research in ARDS. Spontaneous breathing during mechanical ventilation. risks, mechanisms, and management. Am J Respir Crit Care Med. 2017;195:985–92.
Slutsky AS, Ranieri VM. Ventilator-induced lung injury. N Engl J Med. 2013;369:2126–36.
Yoshida T, Nakahashi S, Nakamura MAM, Koyama Y, Roldan R, Torsani V, et al. Volume-controlled ventilation does not prevent injurious inflation during spontaneous effort. Am J Respir Crit Care Med. 2017;196:590–601.
Tonelli R, Fantini R, Tabbì L, Castaniere I, Pisani L, Pellegrino MR, et al. Early inspiratory effort assessment by esophageal manometry predicts noninvasive ventilation outcome in de novo respiratory failure. A pilot study. Am J Respir Crit Care Med. 2020;202:558–67.
Morais CCA, Koyama Y, Yoshida T, Plens GM, Gomes S, Lima CAS, et al. High positive end-expiratory pressure renders spontaneous effort noninjurious. Am J Respir Crit Care Med. 2018;197:1285–96.
Yoshida T, Engelberts D, Chen H, Li X, Katira BH, Otulakowski G, et al. Prone position minimizes the exacerbation of effort-dependent lung injury: exploring the mechanism in pigs and evaluating injury in rabbits. Anesthesiology. 2022;136:779–91.
Pellegrini M, Gudmundsson M, Bencze R, Segelsjö M, Freden F, Rylander C, et al. Expiratory resistances prevent expiratory diaphragm contraction, flow limitation, and lung collapse. Am J Respir Crit Care Med. 2020;201:1218–29.
Moreno F, Lyons HA. Effect of body posture on lung volumes. J Appl Physiol. 1961;16:27–9.
Grieco DL, Maggiore SM, Roca O, Spinelli E, Patel BK, Thille AW, et al. Non-invasive ventilatory support and high-flow nasal oxygen as first-line treatment of acute hypoxemic respiratory failure and ARDS. Intensive Care Med. 2021;47:851–66.
Vilke GM, Chan TC, Neuman T, Clausen JL. Spirometry in normal subjects in sitting, prone, and supine positions. Respir Care. 2000;45:407–10.
Antunes BO, de Souza HCD, Gianinis HH, Passarelli-Amaro RCV, Tambascio J, Gastaldi AC. Peak expiratory flow in healthy, young, non-active subjects in seated, supine, and prone postures. Physiother Theory Pract. 2016;32:489–93.
Katz S, Arish N, Rokach A, Zaltzman Y, Marcus EL. The effect of body position on pulmonary function: a systematic review. BMC Pulm Med BMC Pulmonary Medicine. 2018;18:1–16.
Friedman M, Tanyeri H, La Rosa M, Landsberg R, Vaidyanathan K, Pieri S, et al. Clinical predictors of obstructive sleep apnea. Laryngoscope. 1999;109:1901–7.
Mei LW, Sandham A, Poh KA, Dai CW, Wan CT, Huggare J. Craniofacial morphology, head posture, and nasal respiratory resistance in obstructive sleep apnoea: an inter-ethnic comparison. Eur J Orthod. 2005;27:91–7.
Pelosi P, Tubiolo D, Mascheroni D, Vicardi P, Crotti S, Valenza F, et al. Effects of the prone position on respiratory mechanics and gas exchange during acute lung injury. Am J Respir Crit Care Med. 1998;157:387–93.
Katira BH, Osada K, Engelberts D, Bastia L, Damiani LF, Li X, et al. Positive end-expiratory pressure, pleural pressure, and regional compliance during pronation: an experimental study. Am J Respir Crit Care Med. 2021;126990:1–48.
Goligher EC, Dres M, Patel BK, Sahetya SK, Beitler JR, Telias I, et al. Lung- and diaphragm-protective ventilation. Am J Respir Crit Care Med. 2020;202:950–61.
Mercurio G, Arrigo S, Moroni R, Grieco DL, Menga LS, Romano A, et al. Diaphragm thickening fraction predicts noninvasive ventilation outcome: a preliminary physiological study. Crit Care. 2021;25:219. https://doi.org/10.1186/s13054-021-03638-x.
Goligher EC, Brochard LJ, Reid WD, Fan E, Saarela O, Slutsky AS, et al. Diaphragmatic myotrauma: a mediator of prolonged ventilation and poor patient outcomes in acute respiratory failure. Lancet Respir Med. 2018;2600:1–9.
Grieco DL, Menga LS, Cesarano M, Spadaro S, Bitondo MM, Berardi C, et al. Phenotypes of patients with COVID-19 who have a positive clinical response to helmet noninvasive ventilation. Am J Respir Crit Care Med. 2022;205:360–4.
Grieco DL, Munshi L, Piquilloud L. Personalized noninvasive respiratory support for acute hypoxemic respiratory failure. Intensive Care Med. 2023;49:840–3. https://doi.org/10.1007/s00134-023-07048-1.
Cutuli SL, Grieco DL, Michi T, Cesarano M, Rosà T, Pintaudi G, et al. Personalized respiratory support in ARDS: a physiology-to-bedside review. J Clin Med. 2023;12:4176.
Spinelli E, Mauri T, Fogagnolo A, Scaramuzzo G, Rundo A, Grieco DL, et al. Electrical impedance tomography in perioperative medicine: careful respiratory monitoring for tailored interventions. BMC Anesthesiol. 2019;19:140.
This study was supported by a grant by ESICM (2017 Draeger award) and by Ministero della Saluto (Ricerca corrente 2023).
Ethics approval and consent to participate
The study was approved by local Ethics Committee, and informed consent was obtained by enrolled patients according to committee recommendation.
Consent for publication
DLG has received speaking fees by Gilead, Intersurgical, MSD and GE, and reports having received travel accommodation by Fisher and Paykel. MA has received personal fees by Maquet, and a research grant by Toray. DLG and MA disclose a research grant by GE.
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. E – Figure 1: VAS dyspnea scale.E – Figure 2: VAS discomfort scale. E – Figure 3: Graphic representation of the 4 ROIs and lung contour. In the picture there is a representation of the 32 x 32 matrix provided by the FluxMed® device, after filtering for hearth artifacts and isolating the lungs. In order, from ventral to dorsal: ventral ROI, mid-ventral ROI, mid-dorsal ROI, dorsal ROI. In the analysis, as per manufacturer instructions, the EIT value of each pixel was adjusted to its position in the lung image, with the most peripherical and ventral pixels having the lowest value. E – Figure 4: Graphic representation of the exponential fitted curve in a representative single breath cycle of supine position. The thin gray line represents the raw data with artifacts on which a moving-average smoothing is applied (blue line). The red curve is the negative exponential fit applied (eq. 1). The starting point was chosen as the 75% of the maximum value of the TIV and the end point as the minimum value of the TIV(t).E – Figure 5: Relationship between the change in inspiratory effort and the change in resistances across study phases. In the top two panels, a significant linear relationship is depicted between the increase in respiratory system resistances and inspiratory effort when comparing the supine phases (before and after re-supination) to the prone phase. In the bottom two panels, the same relationship is shown between the change in respiratory system resistances and ΔPL2 across the study phases; however, in this case, the relationship is not significant.
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Grieco, D.L., Delle Cese, L., Menga, L.S. et al. Physiological effects of awake prone position in acute hypoxemic respiratory failure. Crit Care 27, 315 (2023). https://doi.org/10.1186/s13054-023-04600-9
- Acute respiratory failure
- Awake prone position
- Inspiratory effort
- High-flow nasal oxygen
- Patient self-inflicted lung injury