- Research Letter
- Open Access
Early sepsis identification following cytoreductive surgery for peritoneal malignancy
Critical Care volume 24, Article number: 112 (2020)
Cytoreductive surgery for peritoneal malignancy often involves extensive tissue resection, a prolonged operating time, and a significant post-operative systemic inflammatory response (SIRS) . The development of a subsequent post-surgical infection constitutes a common cause of morbidity and mortality, with intra-abdominal infections, in particular, resulting in high mortality rates due to the progression towards tertiary peritonitis and multiple organ dysfunction . The relatively high incidence of infection development, coupled with the immense systemic inflammatory response generated following surgery, makes an early identification of infection problematic and may result in the suboptimal administration of antibiotics.
Current laboratory tools to aid infection diagnosis following surgery include the use of white cell counts (WCC), C-reactive protein (CRP), and procalcitonin (PCT). However, both WCC and CRP levels can be significantly increased in the absence of infection, whilst post-operative PCT concentrations may be heavily dependent on the type and complexity of the surgery performed . Thus, complementary tools to aid diagnosis are required. Previous studies using mid-regional proadrenomedullin (MR-proADM) have highlighted its potential use as an early marker of sepsis development following severe burn injury  due to its involvement in the early stages of capillary leakage, endothelial dysfunction, and multiple organ failure [5, 6]. Accordingly, this biomarker may also be of interest following major surgery.
To test this hypothesis, all patients due to undergo cytoreductive surgery for peritoneal malignancy at the Basingstoke and North Hampshire Hospital were consecutively enrolled between January and December 2017. Kinetic profiles of MR-proADM and CT-proET-1 were compared with those of PCT, WCC, and CRP pre-, intra-, and post-operatively, and for 7 days following surgery.
A total of 50 patients were enrolled with an average operation duration of 7.1 (1.6) h (Table 1). All patients were treated with a combination of Metronidazole and Gentamicin during surgery, whilst 39 (78.0%) were administered vasopressors, 46 (92.0%) treated with intraperitoneal hyperthermic chemoperfusion (HIPEC), and 15 (30.0%) underwent a splenectomy. Furthermore, 15 (30.0%) patients required a blood transfusion during surgery, of which 10 (66.7%) required a follow-up transfusion 3.1 (2.3) days later. A clinical diagnosis of sepsis, defined by the presence of a clinical or radiological infectious focus, a positive pathogen identification and a SOFA score increase of ≥ 2 points, could be confirmed in 4 (8.0%) patients, with 6 (12.0%) additional patients satisfying the same criteria, albeit without a positive pathogen identification. The average time to infection diagnosis was 4.0 (2.1) days following surgery, with additional antibiotics initiated immediately upon diagnosis.
Patients were classified into subgroups based on post-surgical infection development (infected vs. non-infected: N = 10 vs. N = 40; Table 1), with no differences in any biomarker concentration at pre-surgical or intra-surgical time points, or for CT-proET-1 in the subsequent days following surgery (Fig. 1). Significant differences, however, were found between both groups for MR-proADM, PCT, CRP, and WCC at varying time points. MR-proADM concentrations were significantly elevated 1 day (T + 1) after surgery and at all subsequent time points thereafter (infected vs. non-infected: 2.2 [1.5 – 2.5] vs. 1.2 [1.0 – 1.4] nmol/L; p < 0.001), whereas PCT concentrations were significantly elevated 2 days (T + 2) after surgery (infected vs. non-infected: 3.1 [1.4 – 4.5] vs. 0.7 [0.3 – 1.8] ng/mL; p < 0.01). Both CRP and WCC were only significantly elevated at time point T + 3 (p < 0.01). Corresponding AUROC analysis for MR-proADM at T + 1 was 0.90 [0.81 – 1.0] (cut-off: 1.96 nmol/L; sensitivity: 0.90 [0.60 – 0.98]; specificity: 0.85 [0.71 – 0.93]), whereas PCT at T + 2 was 0.77 [0.63 – 0.92] (cut-off: 0.68 ng/mL; sensitivity: 1.0 [0.72 – 1.0]; specificity: 0.48 [0.33 – 0.63]).
Results indicate that MR-proADM kinetics are increased earlier and are more accurate than PCT in identifying patients at risk of developing an infection after cytoreductive surgery for peritoneal malignancy. Additional studies with a larger sample size are required to confirm these hypothesis-generating findings.
Availability of data and materials
All relevant datasets are available from the corresponding author upon reasonable request.
Analysis of variance
Area under the receiver operating characteristic curve
Systemic Inflammatory Response Syndrome
White cell count
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We would like to thank Mr. Tom Cecil and his team in the Peritoneal Malignancy Unit, as well as the Pathology Governance Team and Biochemistry Departments at Hampshire Hospitals NHS Foundation Trust for their continuous support.
The initial study was funded by a restricted grant from Thermo Fisher (Germany). However, the funding organization had no role in the collection, management, analysis, or interpretation of the data, and preparation, review, or approval of the manuscript, or decision to submit the manuscript for publication.
Ethics approval and consent to participate
The study protocol was approved by the ethics board of the Hampshire Hospitals NHS Foundation Trust, and written informed consent obtained from all patients or their legal representatives where appropriate.
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No individual participant data is reported that would require consent to publish from the participant (or legal parent or guardian for children).
All authors have provided information on potential conflicts of interests directly or indirectly related to the work submitted in the journal’s disclosure forms. All authors reported no conflicts of interest.
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Wilson, D.C., Yershov, D., Kandiah, C. et al. Early sepsis identification following cytoreductive surgery for peritoneal malignancy. Crit Care 24, 112 (2020). https://doi.org/10.1186/s13054-020-2831-9