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  • Poster presentation
  • Open Access

Effect of pH levels on platelet aggregation and coagulation: a whole blood in vitro study

  • 1,
  • 1,
  • 1 and
  • 2
Critical Care201115 (Suppl 1) :P446

  • Published:


  • Public Health
  • Blood Sample
  • High Mortality
  • Emergency Medicine
  • Platelet Aggregation


The combination of acidosis, hypothermia and coagulo-pathy is associated with high mortality in polytrauma [1]. Acidosis impairs coagulation [2]. Whether acidosis leads to a reduced platelet function has not so far been evaluated.


In this in vitro study we evaluated the effects of pH levels (7.6, 7.4, 7.2, 7.0 and 6.8) on platelet aggregation and coagulation with human whole blood of healthy male volunteers. We used multiple electrode aggregometry (MEA) Multiplate® (tests: ADP, ASPI, TRAP) for platelet function testing. The global coagulation was evaluated at pH 6.8 and 7.4 with ROTEM®, which is a rotational thrombelastometry (tests: NATEM and APTEM). The pH levels of the blood samples were achieved by titration of HCl and NaOH.


In MEA the AUC was significantly reduced for pH 7.0 and pH 6.8 in all three tests (ADP, ASPI and TRAP), as well as aggregation and velocity. Platelet function was not influenced by alkalosis (pH 7.6). In ROTEM® the AUC, CT, CFT and MCF showed no significant alterations. The α-angle and lysis index for 60 minutes were significantly reduced at pH 6.8. NATEM values were significantly different from those measured with APTEM.


In our study we evaluated a significant decrease of platelet function at pH 7.0 and 6.8 with MEA. The results of the analysis with the ROTEM® system showed a significant reduction of thrombus formation at pH 6.8, as described in the literature. In the APTEM test, we could identify hyperfibrinolysis.

Authors’ Affiliations

Medical University Vienna, Austria
Evangelical Hospital, Vienna, Austria


  1. Cosgriff , et al.: J Trauma. 1997, 42: 857-861. discussion 861-862 10.1097/00005373-199705000-00016View ArticlePubMedGoogle Scholar
  2. Engström , et al.: J Trauma. 2006, 61: 624-628.View ArticlePubMedGoogle Scholar


© Scharbert et al. 2011

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.