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- Open Access
Lipopolysaccharide induces mitochondrial dysfunction in rat cardiac microvascular endothelial cells
© Vuda et al. 2011
- Published: 1 March 2011
- Cellular Respiration
- Mitochondrial Dysfunction
- Mitochondrial Respiration
- Electron Microscopy Examination
- Endothelial Injury
Endothelial injury and dysfunction are key patho-physiological processes in sepsis. The aim of the study was to evaluatethe effects of bacterial lipopolysaccharide (LPS) on cellular respiration of rat primary cardiac microvascular endothelial cells (CMEC).
CMEC were isolated from adult (250 to 300 g) male Wistar rats and cultured. Cells were exposed to LPS (1 μg/ml) for 4, 8, 16 hours and cellular respiration was measured by high-resolution respirometry (Oxygraph-2k; Oroboros Instruments, Innsbruck, Austria). Activation of caspase-3 protein as an early apoptotic event was examined by western blot analysis. Electron microscopy was performed to reveal any alterations in mitochondrial morphology.
The data suggest that prolonged exposure to LPS impairs CMEC complex I-dependent and IV-dependent respiration slightly but significantly, without apparent signs of apoptosis or mitochondrial ultrastructural damage.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.