Skip to main content

Thrombopoietin may enhance ventilator-induced lung injury

Introduction

Ventilator-induced lung injury is characterized by release of inflammatory mediators and increased vascular permeability resulting in alveolar edema formation. Thrombopoietin (TPO), whose most known function is the stimulation of the proliferation of megakaryocytes, has also shown several proinflammatory effects. Moreover, TPO receptor, c-Mpl, is constitutively expressed on endothelial cells and may modulate the permeability of the endothelium. We investigated the role of TPO in the impairment of the alveolar-capillary membrane resulting in alveolar edema formation during mechanical ventilation.

Methods

An ex vivo model of isolated, ventilated and perfused mouse lung was set up: ventilation was performed for 2 hours with both low-stress pressure (peak inspiratory pressure = 7 cmH2O, PEEP = 2 cmH2O, RR = 90 beats/minute) and high-stress pressure (peak inspiratory pressure = 20 cmH2O, PEEP = 0, RR = 90 beats/minute), in the presence or absence of TPO (1 ng/ml) in the perfusate (2% bovine serum albumin RPMI medium at 1 ml/minute flow rate). At the end of the experiment, lung compliance, assessed through tidal volume, and protein concentration in the bronchoalveolar lavage (BAL) fluid were measured.

Results

During high-stress ventilation, lung compliance was significantly reduced by the presence of TPO in the perfusate. TPO did not affect compliance during low-stress pressure. BAL fluid protein concentration was increased by the presence of TPO in both pressure setup, but the increase was statistically significant only after high-stress ventilation. See Table 1.

Table 1 abstract

Conclusions

TPO may enhance the permeability of the alveolar-capillary membrane contributing to the mechanisms of ventilator-induced lung injury.

Author information

Affiliations

Authors

Corresponding author

Correspondence to LD Del Sorbo.

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Cite this article

Del Sorbo, L., Fanelli, V., Muraca, G. et al. Thrombopoietin may enhance ventilator-induced lung injury. Crit Care 15, P207 (2011). https://doi.org/10.1186/cc9627

Download citation

Keywords

  • Protein Concentration
  • Mechanical Ventilation
  • Inflammatory Mediator
  • Tidal Volume
  • RPMI Medium