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- Open Access
In vitro evaluation of HMGB1 removal with various membranes for continuous hemofiltration
© Yumoto et al. 2011
- Published: 1 March 2011
- Clearance Rate
- High Mobility Group
- Loop Circulation
The high mobility group box 1 protein (HMGB1) is an alarmin that plays an important role in sepsis. HMGB1 is hardly removable by normal hemofiltration because of its large molecular weight of 30 kDa. Here we show the possibility of removing HMGB1 from the blood.
The test solution contained 100 μg HMGB1 and 35 g albumin in 1,000 ml of a substitution fluid. Experimental hemofiltration (solution flow of 100 ml/minute and ultrafiltrate flow of 1,000 ml/hour) was conducted for 360 minutes in a closed loop circulation system, and the sieving coefficient (SC) and ultrafiltrate and blood clearance rates of HMGB1 were calculated. High cut-off (HCO), AN69ST, polysulfone (PS), and polymethylmethacrylate (PMMA) membranes were tested (n = 4).
Continuous hemofiltration using HCO or AN69ST membrane will be a promising approach for HMGB1-related sepsis.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.