- Meeting abstract
- Open Access
Impact of carrier solution on biological insulin availability
© Current Science Ltd 2000
- Published: 21 March 2000
- Calculated Concentration
- Time Effect
- Perfusor Rate
- Carrier Solution
- Baseline Sample
When added into all-in-one (AIO) bags, total needs of exogenous insulin decrease compared to amounts given by perfusor. We studied the influence of carrier solution on insulin availability.
Materials and methods
A 20 ml polyvinylchloride (PVC) syringe was filled either with saline or AIO solution aspirated from original 3 l bags (Nutrimix, Brown, Melsungen, Germany). Then 8 IU of insulin (Actrapid HM, NovoNordisk, DK) was added into syringes (calculated concentration 400 IU × l -1) and a PVC perfusion line (5 ml volume) was filled. Baseline samples (0.5 ml) for immunoreactive insulin assay (IRI) were taken. Perfusor rate was set at 2 ml×hr-1 and samples taken directly from the hose at 5, 10, 30, 60, 90 and 210 min, placed on ice and stored at 4°C before analysis. Fifteen sets of measurements were done both for saline and AIO solution. Values are presented as means ± SD. MANOVA, ANOVA for repeated measures and paired T-test with Bonferroni correction were used when appropriate; P<0.05 was considered significant.
Figure 1 shows IRI concentrations in both saline and AIO solutions during the experiment.
Insulin bioavailability in AIO solution is better than in saline with respect to the yield and stability.