- Poster presentation
- Open Access
Interferon-gamma reverses sepsis-induced immunoparalysis of monocytes in vitro
© BioMed Central Ltd. 2010
- Published: 1 March 2010
- Public Health
- Immune Response
- Mononuclear Cell
- Peripheral Blood Mononuclear Cell
- Emergency Medicine
It is currently being understood that most of the agents modulating host response in sepsis have failed because they act to refrain an over-exaggerated immune response whereas immunoparalysis takes place on the time of their administration. The effect of IFNγ on immunoparalysis of monocytes in sepsis was assessed.
Blood was isolated within the first 24 hours from the advent of signs of sepsis from 10 healthy donors and from 33 patients; 14 with sepsis and 19 with severe sepsis/shock. Peripheral blood mononuclear cells (PBMCs) were isolated and stimulated with 10 ng/ml LPS; 5 μg/ml Pam3Cys; and heat-killed isolates of Candida albicans, multidrug-resistant Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA). Stimulations were done in the absence and presence of 10 ng/ml IFNγ. Concentrations of cytokines were estimated in supernatants by an enzyme immunoassay.
IFNγ reverses in vitro sepsis-induced immunoparalysis of monocytes. The type of affected cytokine depends on the agonist and probably reflects the mechanism of action of IFNγ.