- Poster presentation
- Open Access
Phenotypical analysis of peripheral human T lymphocytes in early sepsis
© BioMed Central Ltd. 2010
- Published: 1 March 2010
- Peripheral Blood Mononuclear Cell
- Treg Cell
- Septic Patient
- Single Cell Level
- Intracellular Cytokine
T lymphocytes are crucial immune cells. We analysed T-cell subsets phenotypes and tested, on a single cell level, their ability to produce key cytokines in early human sepsis.
Whole blood was collected from septic patients on ICU admission. Peripheral blood mononuclear cells (PBMC) were isolated and T-cell subsets analysed. To study cytokine production, PMBC were cultured in the presence of PMA/ionomycin (50/750 ng/ml) in supplemented RPMI 1640 for 5 hours. Intracellular cytokines IL-4, IL-10, IL-17, IFNγ were stained in CD3+/CD4+, CD3+/CD8+ cells using flow cytometry for both. The number of cytokine producing cells was compared with age/sex-matched healthy human volunteers. Data are expressed as mean ± SEM.
Our results confirm a relative increase of Treg  and a skew towards Th2 lineage in the CD4+ cells. The highly activated CD8+ cells appear to be more susceptible to activation-induced cell death.