Volume 13 Supplement 3

Fifth International Symposium on Intensive Care and Emergency Medicine for Latin America

Open Access

Feasibility of stored red blood cell transfusion in pigs

  • LCP Azevedo1,
  • S Biagini1,
  • PA Costa1,
  • AL Rosário1,
  • SP Schettino1,
  • S Wendel1 and
  • LC Azevedo1
Critical Care200913(Suppl 3):P2

https://doi.org/10.1186/cc7804

Published: 23 June 2009

Introduction

The mechanisms associated with immunomodulation after red blood cell transfusion are not completely understood, possibly due to methodological biases in the clinical studies and presence of comorbidities such as sepsis. Therefore, a controlled animal model of blood cell transfusion may be a more appropriate approach to minimize these issues. We designed this pilot study in order to validate in vitro and in vivo the survival of swine erythrocytes stored for 13 days.

Methods

Blood was collected from one Agroceres® swine and stored in 2 units of red blood cells (RBC). The following measurements were performed at baseline and after 13 days of storage: volume, hemoglobin and hematocrit, hemolysis index, potassium, sodium, glucose and pH. In vivo validation and hemolysis evaluation were performed by labeling the cells with Na251CrO4 and recovering viable erythrocytes up to 24 hours after transfusion in one autologous material and four homologous animals. A splenectomy was performed after death to evaluate splenic sequestration of RBC.

Results

In vitro validation of the samples is demonstrated in Table 1. The mean RBC recovery value after 24 hours of injection of labeled RBC was 97.5 ± 19%, demonstrating a good viability of the samples. The evaluation of splenic hemolysis was negative.
Table 1

abstract P2

Unit

Period

Volume (ml)

HT (%)

Hb (g/dl)

Hemolysis index (%)

K+ (mEq/l)

Na+ (mEq/l)

pH

Glucose (mg/dl)

Unit 1

Baseline

196.3

68.8

22.5

0.07

3.5

144

7.09

333

 

13-day storage

187.1

67.9

22.3

0.09

32.8

118

6.90

362

Unit 2

Baseline

163

67.9

22.3

0.02

3.2

145

6.96

362

 

13-day storage

144.7

67.2

22

0.1

31.3

120

6.90

370

Conclusion

Erythrocytes from pigs stored under human standardized conditions for up to 13 days may be used for experimental transfusion studies. This controlled animal model may be useful to study pathogenetic mechanisms related to adverse effects of RBC transfusion.

Declarations

Acknowledgements

Supported by Research and Education Institute, Hospital Sírio-Libanês.

Authors’ Affiliations

(1)
Research and Education Institute, Hospital Sírio-Libanês

Copyright

© BioMed Central Ltd 2009

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