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Effects of mercaptoethylguanidine during long-term hyperdynamic porcine endotoxemia

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Introduction

Excess NO production due to iNOS activation and cellular toxicity resulting from peroxynitrite (ONOO-) may contribute to organ dysfunction in septic shock. Therefore, we studied the effect of the combined ONOO-scavenger and selective iNOS-inhibitor mercaptoethylguanidine (MEG) [1] on hepato-splanchnic hemodynamics and energy metabolism during long-term hyperdynamic porcine endotoxemia [2].

Methods

12 h after starting continuous intravenous endotoxin, pigs received either no drug (CNT; n=9) or 3 mg/kg/h MEG (n=7). Hydroxyethyl starch was infused to maintain a sustained increase in cardiac output [2]. Before, as well as 12, 18, and 24 h after, the start of LPS we assessed expired NO formation (chemiluminescence), systemic (CO) and liver (Doppler ultrasound flow probes) blood flow, arterial-ileal mucosal PCO2-gap (fiberoptic sensor), portal (pv) and hepatic venous (hv) lactate/pyruvate (L/P) ratios and hepatic lactate clearance.

Results

See Table.

Conclusion

MEG allowed for hemodynamic stabilization due to blunting of the progressive endotoxin-induced fall in MAP while maintaining CO but did not influence the parameters of hepato-splanchnic energy metabolism. Ongoing oxidative stress resulting from inadequate dosage of the compound may account for this result [3].

Table 1

References

  1. 1.

    Szabó , et al.: . Crit Care Med 1999, 27: 1353.

    Article  Google Scholar 

  2. 2.

    Santak , et al.: . Br J Pharmacol 1998, 124: 1689-1697. 10.1038/sj.bjp.0701998

    PubMed  CAS  PubMed Central  Article  Google Scholar 

  3. 3.

    Whiteman , et al.: . Br J Pharmacol 1999, 126: 1646-. 10.1038/sj.bjp.0702465

    PubMed  CAS  PubMed Central  Article  Google Scholar 

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Acknowledgement

Supported by Provinz Bozen-Südtirol (Italy), ESICM and Deutsche Forschungsgemeinschaft.

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Ploner, F., Tugtekin, I., Matejovic, M. et al. Effects of mercaptoethylguanidine during long-term hyperdynamic porcine endotoxemia. Crit Care 4, P51 (2000). https://doi.org/10.1186/cc771

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Keywords

  • Peroxynitrite
  • Hydroxyethyl
  • Hemodynamic Stabilization
  • Hydroxyethyl Starch
  • Flow Probe