The brain is a major source of S100 increase in porcine endotoxemic shock
© Lipcsey et al; licensee BioMed Central Ltd. 2009
Published: 13 March 2009
Cerebral dysfunction frequently complicates septic shock. A marker of cerebral dysfunction could be of significant value in managing sedated septic patients. Plasma S100 (S100B) proteins increase in sepsis. S100B is present in the brain, but also in other tissues. To date, the source of this protein has not been investigated in sepsis. The aim of this study is to determine whether the brain is the source of S100B in an experimental sepsis model.
Twenty-seven pigs were anesthetized and randomized to either infusion of endotoxin at the rate of 1 μg/kg/hour (n = 19) or saline (n = 8). Catheters were inserted into a cervical artery and the superior sagittal sinus. Blood samples were collected from both sites and physiological data were registered before the start of the endotoxin infusion and hourly. After 6 hours, the animals were terminated and brain tissue samples were taken from the left hemisphere. S100B in plasma was measured by ELISA. Brain tissue samples were stained with biotinylated S100B antibodies.
Although other sources exist, the brain is a major source of S100B in endotoxemia, making it a potential marker of cerebral dysfunction in septic shock.
This article is published under license to BioMed Central Ltd.