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  • Poster presentation
  • Open Access

Kinetic study of gut and systemic tissue perfusion following one challenge of bacterial translocation

  • 1,
  • 2,
  • 3,
  • 2,
  • 1,
  • 1,
  • 2 and
  • 1
Critical Care200711 (Suppl 3) :P25

  • Published:


  • Emergency Medicine
  • Organ Failure
  • Kinetic Study
  • Multiple Organ
  • Sham Group


The pathogenesis of sepsis and multiple organ failure has been associated with bacterial translocation (BT). In a previous study we observed intestinal and systemic tissue hypoperfusion 2 hours after a BT process. In this study we examined the perfusion kinetics a longer period after one unique challenge of BT.

Materials and methods

Adult female Wistar rats (± 200 g) were submitted to the induction of 2 hours of BT (E. coli R6 1010 CFU/ml, 5 ml/100 g weight by oroduodenal catheterization). Sham groups received saline. The tissue perfusion (jejunum, ileum, liver and right and left kidneys) was monitored before BT and 2, 6, 24 and 72 hours, 7 and 14 days after BT (n = 6/group).

Results and discussion

The tissue perfusions in BT groups were statistically decreased at 2 and 24 hours in all organs, returning to normal levels after 72 hours up to 14 days compared with sham groups, except the ileum that remained with a high perfusion index after 72 hours onward. Interestingly, in the 6 hours BT group a transitory increased perfusion occurred in all organs, being significant at gut tissues, denoting that at this time point transient inflammatory-response-dependent vasodilatation might have occurred (Figure 1). The BT-related hypoperfusion effect seems to be related to a BT-induced host inflammatory response.
Figure 1
Figure 1

Mean tissue perfusion units (Δ%) of sham and BT groups.


Single BT challenge provoked significant and enduring local and systemic tissue hypoperfusion. These findings can support the hypothesis of BT-related sepsis aggravation.

Authors’ Affiliations

Department of Surgery, Federal University of São Paulo, SP, Brazil
Department of Pediatrics, Federal University of São Paulo, SP, Brazil
Department of Infectology, Federal University of São Paulo, SP, Brazil


© BioMed Central Ltd 2007