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Kinetic study of gut and systemic tissue perfusion following one challenge of bacterial translocation

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Introduction

The pathogenesis of sepsis and multiple organ failure has been associated with bacterial translocation (BT). In a previous study we observed intestinal and systemic tissue hypoperfusion 2 hours after a BT process. In this study we examined the perfusion kinetics a longer period after one unique challenge of BT.

Materials and methods

Adult female Wistar rats (± 200 g) were submitted to the induction of 2 hours of BT (E. coli R6 1010 CFU/ml, 5 ml/100 g weight by oroduodenal catheterization). Sham groups received saline. The tissue perfusion (jejunum, ileum, liver and right and left kidneys) was monitored before BT and 2, 6, 24 and 72 hours, 7 and 14 days after BT (n = 6/group).

Results and discussion

The tissue perfusions in BT groups were statistically decreased at 2 and 24 hours in all organs, returning to normal levels after 72 hours up to 14 days compared with sham groups, except the ileum that remained with a high perfusion index after 72 hours onward. Interestingly, in the 6 hours BT group a transitory increased perfusion occurred in all organs, being significant at gut tissues, denoting that at this time point transient inflammatory-response-dependent vasodilatation might have occurred (Figure 1). The BT-related hypoperfusion effect seems to be related to a BT-induced host inflammatory response.

Figure 1
figure1

Mean tissue perfusion units (Δ%) of sham and BT groups.

Conclusion

Single BT challenge provoked significant and enduring local and systemic tissue hypoperfusion. These findings can support the hypothesis of BT-related sepsis aggravation.

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Vilela-Oliveira, L., Menchaca-Diaz, J., Salomão, R. et al. Kinetic study of gut and systemic tissue perfusion following one challenge of bacterial translocation. Crit Care 11, P25 (2007) doi:10.1186/cc5812

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Keywords

  • Emergency Medicine
  • Organ Failure
  • Kinetic Study
  • Multiple Organ
  • Sham Group