Volume 11 Supplement 3

Fourth International Symposium on Intensive Care and Emergency Medicine for Latin America

Open Access

Kinetic study of gut and systemic tissue perfusion following one challenge of bacterial translocation

  • L Vilela-Oliveira1,
  • JL Menchaca-Diaz2,
  • R Salomão3,
  • AMA Liberatore2,
  • MY Taki1,
  • J FranciscoJr1,
  • U Fagundes-Neto2 and
  • IHJ Koh1
Critical Care200711(Suppl 3):P25


Published: 19 June 2007


The pathogenesis of sepsis and multiple organ failure has been associated with bacterial translocation (BT). In a previous study we observed intestinal and systemic tissue hypoperfusion 2 hours after a BT process. In this study we examined the perfusion kinetics a longer period after one unique challenge of BT.

Materials and methods

Adult female Wistar rats (± 200 g) were submitted to the induction of 2 hours of BT (E. coli R6 1010 CFU/ml, 5 ml/100 g weight by oroduodenal catheterization). Sham groups received saline. The tissue perfusion (jejunum, ileum, liver and right and left kidneys) was monitored before BT and 2, 6, 24 and 72 hours, 7 and 14 days after BT (n = 6/group).

Results and discussion

The tissue perfusions in BT groups were statistically decreased at 2 and 24 hours in all organs, returning to normal levels after 72 hours up to 14 days compared with sham groups, except the ileum that remained with a high perfusion index after 72 hours onward. Interestingly, in the 6 hours BT group a transitory increased perfusion occurred in all organs, being significant at gut tissues, denoting that at this time point transient inflammatory-response-dependent vasodilatation might have occurred (Figure 1). The BT-related hypoperfusion effect seems to be related to a BT-induced host inflammatory response.
Figure 1

Mean tissue perfusion units (Δ%) of sham and BT groups.


Single BT challenge provoked significant and enduring local and systemic tissue hypoperfusion. These findings can support the hypothesis of BT-related sepsis aggravation.

Authors’ Affiliations

Department of Surgery, Federal University of São Paulo
Department of Pediatrics, Federal University of São Paulo
Department of Infectology, Federal University of São Paulo


© BioMed Central Ltd 2007