Sepsis provokes host's microbiota overgrowth of commensal Gram-negative bacteria and subsequent induction of bacterial translocation in rats
© BioMed Central Ltd 2007
Published: 19 June 2007
The literature has shown the participation of intestinal microbiota in the genesis of primary infections as well as of sepsis. In this study we examine the role of sepsis on the microbiota by examining the most frequently recovered Gram-negative bacteria (G-).
Materials and methods
Adult Wistar rats (± 200 g) were submitted to the induction of semi-lethal sepsis (S-G) (E. coli R6 1 ml of 108 CFU/ml/100 g body weight, i.v.). Firstly, fecal G – kinetic following sepsis induction was examined (6, 12, 24, 48, 72, 120 and 216 hours) (n = 6). After sepsis induction, in other groups (n = 18), samples were harvested from the small bowel (duodenum, jejunum, ileum) and large bowel (cecum and feces before and after sepsis) at 6, 12 and 24 hours, and the BT index was examined at the mesenteric lymph nodes (MLN), liver and spleen by culture in MacConkey medium. Control groups were the sham group (Sham-G, saline injection) (n = 18) and the naïve group (N-G, without any procedure) (n = 6).
Sepsis provoked G – overgrowth and this was able to induce the BT process. Other factors, such as splanchnic hypoperfusion, decreased peristalsis and gut immunity by sepsis, might have also contributed to this event.