Volume 11 Supplement 3

Fourth International Symposium on Intensive Care and Emergency Medicine for Latin America

Open Access

Effects of hypertonic saline solution and pentoxifylline on rat mesenteric microcirculation after hemorrhagic shock/reperfusion followed by cecal ligation/puncture: an intravital microscopic study

  • NK Nakagawa1, 2,
  • RA Nogueira1,
  • CA Obuti2,
  • CJ Correia1,
  • JWMC Cruz3,
  • LF Poli de Figueiredo1,
  • M Rocha e Silva1 and
  • P Sannomiya1
Critical Care200711(Suppl 3):P7

https://doi.org/10.1186/cc5794

Published: 19 June 2007

Objective

Hemorrhagic shock/reperfusion followed by sepsis triggers systemic microcirculatory disturbances that may induce multiple organ failure. The present in-vivo study evaluated the effects of hypertonic saline solution (HSS) (7.5%, 4 ml/kg) and pentoxifylline (PTX) (4 mg/kg) on mesenteric microcirculation in double-injured rats.

Methods

Thirty-three anesthetized Wistar rats (~250 g) were randomly assigned to: (1) SHAM: no injury group; (2) HSS: hemorrhagic shock/reperfusion with HSS; (3) LR: hemorrhagic shock/reperfusion with lactated Ringer's solution (LR), three times shed blood volume; (4) HSS + PTX: hemorrhagic shock/reperfusion with HSS associated with PTX; and (5) LR + PTX: hemorrhagic shock/reperfusion with LR associated with PTX. The animals were submitted to cecal ligation/puncture (second injury).

Results

Leukocyte – endothelium interactions (Table 1) were assessed by intravital microscopy at mesenteric postcapillaric venules (~21.07 μm diameter).
Table 1

Leukocyte – endothelial interactions in mesenteric postcapillaric venules

  

Rollers

Adherent cells

Migrated cells

Total white blood

Group

n

(10 min)

(100 μm venule length)

(5,000 μm2)

(cells/mm3)

SHAM

6

96 ± 12

3 ± 1

2 ± 1

12,367 ± 2,641

LR

7

207 ± 19a

17 ± 1a

17 ± 1a

16,771 ± 5,703a

HSS

8

108 ± 16

9 ± 3c

12 ± 3c

12,388 ± 6,629

HSS + PTX

7

102 ± 26

6 ± 1b,c

6 ± 1b,c

10,757 ± 2,483

LR + PTX

5

115 ± 20

7 ± 1c

7 ± 1b,c

11,650 ± 1,570

a P < 0.001 vs other groups, c P < 0.001 vs SHAM, c P < 0.001 vs HSS.

Conclusion

The in-vivo observation of the rat mesenteric microcirculation showed that, in a double-injury model, reperfusion of the animals with HSS and PTX did attenuate the inflammation response compared with LR-reperfused animals.

Declarations

Acknowledgements

Funded by FAPESP and PRONEX.

Authors’ Affiliations

(1)
Research Division, Heart Institute (InCor), LIM-11 University of São Paulo Medical School
(2)
UNICID
(3)
Institute of Biomedical Sciences, University of São Paulo

Copyright

© BioMed Central Ltd 2007

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