Volume 11 Supplement 3

Fourth International Symposium on Intensive Care and Emergency Medicine for Latin America

Open Access

Effects of hypertonic saline and lactated Ringer's solutions on bacterial translocation in a rat model of intestinal obstruction and ischemia

  • FL Zanoni1,
  • KV Greco1,
  • ACR Moreno2,
  • LF Poli de Figueiredo1,
  • MR Silva1 and
  • P Sannomiya1
Critical Care200711(Suppl 3):P4

https://doi.org/10.1186/cc5791

Published: 19 June 2007

Introduction

Clinical evidence suggests that bacterial translocation (BT) may not be the primary cause in the development of sepsis and multiple organ dysfunction. However, BT has an important role in the activation of the immune system. Therapies have been extensively investigated to improve tissue perfusion and reduce intestinal ischemia. The aim of this study is to evaluate the effects of hypertonic saline (HSS) 7.5% and lactated Ringer's (LR) solutions on intestinal BT in rats that underwent intestinal obstruction and ischaemia (IO).

Methods

Wistar rats (300 ± 50 g) underwent anesthesia with sodium pentobarbital (50 mg/kg, i.p.) and were submitted to IO: (i) cecum exposure, (ii) ileum ligation at 1.5 cm proximal to the ileocecal valve, and (iii) ligation of the mesenteric vessels that supply a 7–10 cm length of the ileal loop. Two hours after surgical procedures, 4 ml/kg of 7.5% HSS or LR were administered intravenously, during 5 minutes. Animals were killed 24 hours after IO, and microbiological assays were performed in mesenteric lymph nodes, liver, spleen, and blood.

Results

See Table 1.
Table 1

Microbiological assays

 

Mesenteric lymph nodes

Liver

Spleen

Blood

Group

+/n

CFU/g

+/n

CFU/g

+/n

CFU/g

+/n

Sham

1/7

57

0/7

NG

0/7

NG

0/7

IO

6/7

2,939 ± 1,751

6/7

953 ± 525

6/7

4,616 ± 1,973

4/7

LR

7/7

1,862 ± 1,178

5/7

3,080 ± 1,832

6/7

4,376 ± 2,836

6/7

HSS

6/7

2,371 ± 1,451

3/7

104 ± 67

4/7

174 ± 75

1/7

Sham group, false operated; +/n, number of animals with positive cultures for E. coli/total number of animals; CFU/g, colony formation units/g tissue (mean value ± SEM, n = 7 animals in each group); NG, no growth.

Conclusion

HSS reduced the number of CFU/g in the liver, spleen, and blood after IO, resulting in improvement of the 'gut barrier function'.

Authors’ Affiliations

(1)
Research Division, Heart Institute (InCor), LIM 11, University of São Paulo Medical School
(2)
Faculty of Pharmaceutical Sciences, Department of Clinical Analysis, University of São Paulo

Copyright

© BioMed Central Ltd 2007

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