- Poster presentation
Ethyl pyruvate prevents acute lung injury in an experimental multitrauma model
Critical Care volume 10, Article number: P179 (2006)
Ethyl pyruvate (EP) is a pyruvate derivative that has been reported to improve survival, to decrease proinflammatory cytokine expression (including high mobility group box-1) and to ameliorate organ dysfunction in animals who have lethal sepsis or were subjected to hemorrhagic shock. We examined the potential protective effects of EP administered after multi-trauma on lung oxidative damage and apoptosis in a rat model with delayed resuscitation.
Materials and methods
Thirty-two male Wistar rats were equally divided into sham-control, multi-trauma, EP and multi-trauma + EP treatment groups. Anesthesia was performed with ketamine hydrochloride (60 mg/kg, intramuscularly) in all groups. Multi-trauma was applied as a moderate head trauma, left femur and tibia fractures under anesthesia. Head trauma was created using impaction model; a 450 g weight was dropped on to a metal plate fixed to the head of the subjects from a 1 m height through a Plexiglas guide tube . The fractures of the tibia and femur were created by dropping a blunt guillotine with a weight of 500 g . The first and third groups were resuscitated with Ringer lactate solution. EP (as a Ringer ethyl pyruvate solution; Sigma) was administered 40 mg/kg intraperitoneally 6 hours after the multi-trauma and animals were sacrificed at 24 hours. Post-trauma treatment with EP after the multi-trauma prevented the increase in lung tissue TBARS levels and serum MPO levels (Figs 1 and 2; P < 0.05). Lung tissue histopathology demonstrated a dramatic reduction in neutrophil infiltration and caspase-stained cells in the multi-trauma + EP group.
Discussion and conclusion
These results suggest that a single dose of EP inhibits leukocyte infiltration and oxidative lung damage, even when given 6 hours after the multi-trauma. EP warrants further evaluation as a therapeutic agent to ameliorate multi-traumainduced acute lung injury.
Foda MA, Marmarou A: A new model of diffuse brain injury in rats. Part II: morphological characterization. J Neurosurg 1994, 80: 301-313. 10.3171/jns.1994.80.2.0301
Bonnarens F, Einhorn TA: Production of a standard closed fracture in laboratory animal bone. J Orthop Res 1984, 2: 97-101. 10.1002/jor.1100020115
About this article
Cite this article
Cinel, I., Oztuna, V., Karabacak, T. et al. Ethyl pyruvate prevents acute lung injury in an experimental multitrauma model. Crit Care 10, P179 (2006). https://doi.org/10.1186/cc4526
- Acute Lung Injury
- Ethyl Pyruvate
- Proinflammatory Cytokine Expression
- Ringer Lactate Solution
- Lethal Sepsis