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Assessment of intravascular retention of hydroxyethyl starch in mild hemorrhagic shock


To evaluate the retention of hydroxyethyl starch (HES) in the vessel at the microcirculation using intravital microscopy [1, 2].


Experiments were performed using a mild hemorrhagic (10% of total blood volume) shock model in rats. The blood vessels (A2 and V2) of the cremaster muscle were observed. One hour after hemorrhage was induced, FITC-labeled L-HES (MW 150,000–200,000) or M-HES (MW 175,000–225,000) or H-HES (MW 550,000–850,000) was administered within 15 min. The retention of HES was evaluated by the contrasting density of the brightness of fluorescence on the image stored on the PC.


A retention ratio of HES inside the blood vessel was different among groups. M-HES and H-HES retained in the vessels longer than L-HES, with no difference between M-HES and H-HES (Fig. 1).

Figure 1
figure 1

(abstract P172)


M-HES (MW 175,000–225,000) seems to have large molecules, which is enough to maintain the blood volume after hemorrhage.


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Hitosugi, T., Shimizu, T., Suzuki, S. et al. Assessment of intravascular retention of hydroxyethyl starch in mild hemorrhagic shock. Crit Care 10 (Suppl 1), P172 (2006).

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