- Open Access
Lactate concentration gradient from right atrium to pulmonary artery
Critical Care volume 9, Article number: R425 (2005)
We compared simultaneous measurements of blood lactate concentration ([Lac]) in the right atrium (RA) and in the pulmonary artery (PA). Our aim was to determine if the mixing of right atrial with coronary venous blood, having substantially lower [Lac], results in detectable decreases in [Lac] from the RA to the PA.
A prospective, sequential, observational study was conducted in a medical-surgical intensive care unit. We enrolled 45 critically ill adult individuals of either sex requiring pulmonary artery catheters (PACs) to guide fluid therapy. Immediately following the insertion of the PAC, one paired set of blood samples per patient was drawn in random order from the PAC's proximal and distal ports for measurement of hemoglobin concentration, O2 saturation (SO2) and [Lac]. We defined Δ[Lac] as ([Lac]ra - [Lac]pa), ΔSO2 as (SraO2 - SpaO2) and the change in O2 consumption (ΔVO2) as the difference in systemic VO2 calculated using Fick's equation with either SraO2 or SpaO2 in place of mixed venous SO2. Data were compared by paired Student's t-test, Spearman's correlation analysis and by the method of Bland and Altman.
We found SraO2 > SpaO2 (74.2 ± 9.1 versus 69.0 ± 10.4%; p < 0.001) and [Lac]ra > [Lac]pa (3.9 ± 3.0 versus 3.7 ± 3.0 mmol.l-1; p < 0.001). Δ[Lac] correlated with ΔVO2 (r2 = 0.34; p < 0.001).
We found decreases in [Lac] from the RA to PA in this sample of critically ill individuals. We conclude that parallel decreases in SO2 and [Lac] from the RA to PA support the hypothesis that these gradients are produced by mixing RA with coronary venous blood of lower SO2 and [Lac]. The present study is a preliminary observation of this phenomenon and further work is needed to define the physiological and clinical significance of Δ[Lac].
Pulmonary artery (PA) blood comprises the mixed venous effluent from all organs, with the notable exception of the lungs. PA O2 saturation (SpaO2) has been promoted as an index of tissue oxygenation [1, 2] because it is thought to be related to the average end capillary blood PO2 .
In a prior study , we measured the O2 saturation (SO2) of right atrial blood (SraO2) and SpaO2 in samples drawn from the proximal and distal ports of PA catheters (PACs) placed in critically ill patients. We noted that SpaO2 was consistently lower than SraO2 by approximately 5%. Others have noted a similar step-down in O2 saturation from the right atrium (RA) to the PA [5, 6], and continuous measurements in critically ill patients have shown a similar difference between SpaO2 and central venous (CV) O2 saturation (ScvO2) of approximately 7% .
The RA to PA O2 saturation gradient (defined as ΔSO2 = SraO2 - SpaO2) is likely the result of mixing atrial blood with highly desaturated blood entering the right heart chambers from the coronary veins. This includes blood flowing from the coronary sinus, the great cardiac vein and other major epicardial veins.
As a result of myocardial lactate extraction from the coronary circulation, the CV lactate concentration ([Lac]cv) is the lowest of any venous blood [8, 9]. In the present study we compare blood lactate concentration ([Lac]) in paired samples drawn from the proximal and distal ports of PACs placed in critically ill patients ([Lac]ra and [Lac]pa) to establish whether we could also detect a decreasing lactate concentration gradient from right atrium to pulmonary artery (Δ[Lac] = [Lac]ra - [Lac]pa).
This was a prospective, sequential study performed in the George Washington University Hospital intensive care unit. The George Washington University Institutional Review Board approved the study and informed consent was obtained from the patient or from the next of kin.
The data presented were culled from a subset of patients enrolled in a previous study . We enrolled individuals older than 18 years of age of either sex in whom their physicians determined that a PAC was required to guide fluid therapy. Enrollment in the study occurred at the time the patient or the nearest relative consented to the introduction of the PAC. On the basis of their medical history, we excluded patients with uncorrected valvular incompetence, intra-cardiac shunting or those who required insertion of the pulmonary artery catheter through the femoral vein.
A 7.5 French, 5 lumen, 110 cm length, PAC with the right atrial lumen positioned 30 cm from the tip (Edwards Lifesciences, Irvine, CA, USA) was inserted through the internal jugular vein or the subclavian vein using a percutaneous sheath introducer (8.5 French; Edwards Lifesciences). The insertion technique is described elsewhere . Care was taken to place the distal port catheter in the PA and the proximal port in the RA.
Immediately after the insertion of the PA catheter, each patient had one set of paired blood samples drawn in rapid succession, and in random order, from the proximal and distal port. We took proximal port blood to be representative of RA blood, whereas distal port blood was considered to be PA blood. The first 2 ml of blood drawn for each sample were discarded to prevent contamination with flushing fluid. Blood samples were drawn with the catheter balloon deflated to avoid contamination of the distal port sample with pulmonary capillary blood. Arterial O2 saturation was determined from a previously in vivo calibrated pulse oximeter.
Blood samples were placed on ice and taken to a central laboratory for measurement of [Lac] (Ektachem 950 IRC Chemistry Analyzer with a Vitros Products lactate slide, Ortho-Clinical Diagnostic, Inc., Rochester, NY, USA), hemoglobin concentration ([Hb]) and O2 saturation (ABL700 Radiometer America Inc., Westlake, OH, USA). We measured cardiac output (CO) by the thermodilution method as the average of three sequential determinations.
Systemic O2 delivery (DO2), O2 consumption (VO2), O2 extraction ratio (ERO2), double product (DP; heart rate (HR) × mean arterial pressure (MAP)) and left ventricular stroke work index (LVSWI) were computed using standard formulae. We defined ΔVO2 as the difference in systemic VO2 calculated with Fick's equation with either SpaO2 or SraO2 in place of the mixed venous SO2 (SvO2); ΔVO2 = Qpa × 13.4 × [Hb] × (SraO2 - SpaO2) ml.min-1.
Paired Student's t-test was used to compare atrial to PA measurements. [Lac]ra and [Lac]pa were compared by Spearman's correlation analysis . The method of Bland and Altman  was used to investigate the effect of lactate concentration on the differences between paired observations. The relationships between Δ[Lac] and ΔSO2, ΔVO2 and other hemodynamic parameters were analyzed by Spearman's correlation analysis. Data are shown as mean ± SD with p < 0.05 denoting a significant difference.
We enrolled 45 patients in the study, including 18 women. The study group was composed of 31 post-operative patients (26 post-cardiac surgery), 11 patients in septic shock from various medical conditions, 2 patients with severe gastrointestinal bleeding and 1 patient in congestive heart failure. Demographic and hemodynamic parameters for the group are listed in Table 1.
The mean SO2 and lactate concentrations for RA and PA blood samples are shown in Table 2. SraO2 was greater than SpaO2 (p < 0.001), with ΔSO2 = 5.2 ± 4.8%. [Lac]ra was greater than [Lac]pa (p < 0.001), with Δ[Lac] = 0.2 ± 0.2 mmol.l-1.
Shown in Fig. 1 is a Bland-Altman plot comparing [Lac]ra and [Lac]pa. There was a bias towards greater [Lac]ra of 0.2 mmol.l-1 (p < 0.001) with a 95% confidence interval for the population of -0.15 to 0.56 mmol.l-1. There was no discernable relationship between [Lac]ra and Δ[Lac] (r2 = 0.03; p = 0.33), indicating that Δ[Lac] was not a concentration dependent phenomenon. Moreover, we found no significant relationships between [Lac]ra and SraO2 or between [Lac]pa and SpaO2.
There was a significant relationship between Δ[Lac] and ΔVO2 (Δ[Lac] mmol.l-1 = 0.0026 ΔVO2 ml.min-1 + 0.0975; r2 = 0.34; p < 0.0001) with a standard error of the estimate of 0.15 mmol.l-1 (Fig. 2). There were no significant correlations between Δ[Lac] and cardiac index, DP, LVSWI, DO2, VO2 or ERO2.
We detected a decreasing Δ[Lac] when comparing paired blood samples drawn from the proximal and distal ports of PACs. We also noted Δ[Lac] correlated with ΔVO2. To our knowledge, these novel findings have not been reported elsewhere.
Only one other study in the literature has compared central venous [Lac] to [Lac]pa. This study found no differences in [Lac], although it was biased by the use of multiple blood samples (n = 50) drawn from 12 critically ill patients . Our study used only one comparison per subject, which perhaps may explain the difference in results.
We used a standard clinical laboratory instrument to measure [Lac] having a 95% precision of ± 0.1 mmol.l-1. Even assuming a worst case scenario of a systematic instrument bias of -0.1 mmol.l-1, the difference in [Lac] between RA and PA would have remained statistically significant.
The declining [Lac] gradient from RA to PA is likely the result of mixing RA blood with blood of lower [Lac] emanating from the coronary venous system. Lactate oxidation accounts for 10% to 20% of total myocardial aerobic energy production , a proportion that increases substantially in sepsis . As a result of myocardial lactate extraction, coronary venous [Lac] is substantially lower than arterial [Lac] and is the lowest of all venous effluents . The dilution of RA blood by coronary venous blood of lower [Lac] is a plausible explanation for the small but detectable difference in [Lac] from RA to PA.
Since RA blood is the mixture of superior vena cava and inferior vena cava (IVC) blood, the possibility exists that these blood streams had not thoroughly mixed at the proximal PAC sampling port. In this case, one could expect further mixing to occur between IVC and RA blood while flowing into the pulmonary artery. Our results do not support this hypothesis. Direct measurements in humans show that IVC blood has the highest [Lac] of any major vein  and further mixing of RA with IVC blood would have produced higher, not lower, [Lac]pa. A factual resolution of this question can only be achieved by direct measurement of [Lac] from IVC to PA.
Only three individuals in our group had [Lac]ra < [Lac]pa. These patients had no distinguishing features to help us differentiate them from others in the group. It is possible that accidental mislabeling of the samples may have accounted for a negative Δ[Lac] but we think it unlikely, given the care taken with the labeling and measuring of the samples. Another possibility is that these individuals experienced myocardial ischemia, a condition associated with an upsurge in glucose metabolism and net lactate release by the heart [17–19]. Myocardial lactate release, as opposed to the normal state of myocardial uptake, would have resulted in [Lac]ra < [Lac]pa.
Others have noted a linear relationship between myocardial O2 consumption (MVO2) and myocardial lactate uptake, reflecting the O2 cost of lactate utilization by the heart . We did not measure MVO2 directly but calculated ΔVO2, a parameter denoting the difference in systemic VO2 prior to and immediately after entry of myocardial effluent blood into the venous circulation. As such, ΔVO2 bears a direct relationship to MVO2. We noted a linear relationship between ΔVO2 and Δ[Lac] (Fig. 2) similar to that described between MVO2 and myocardial lactate uptake. This finding suggests that Δ[Lac] also may be related, in a yet to be established fashion, to MVO2.
We found decreases in [Lac] from RA to PA in this sample of critically ill individuals. We conclude that parallel decreases in SO2 and [Lac] from RA to PA support the hypothesis that these gradients are produced by mixing RA with coronary venous blood of lower SO2 and [Lac]. The present study is a preliminary observation of this phenomenon and further work is needed to define the physiological and clinical significance of Δ[Lac].
Oxygen and lactate concentrations are lower in PA blood than in RA blood.
The oxygen and lactate concentration gradients from RA to PA are likely the result of mixing atrial with coronary venous blood.
The possibility exists that these concentration gradients may reflect changes in myocardial energy requirements.
central venous pressure
systemic O2 delivery
oxygen extraction ratio
inferior vena cava
lactate concentration gradient from right atrium to pulmonary artery
blood lactate concentration
left ventricular stroke work index
mean arterial pressure
mean pulmonary pressure
myocardial O2 consumption
pulmonary artery catheter
pulmonary artery occlusion pressure
O2 saturation gradient from right atrium to pulmonary artery
systemic vascular resistance index
Vincent JL: The relationship between oxygen demand, oxygen uptake, and oxygen supply. Intensive Care Med 1990,16(suppl 2):S145-S148. 10.1007/BF01785244
Gutierrez G, Wulf-Gutierrez M, Reines HD: Monitoring oxygen transport and tissue oxygenation. Curr Opin Anes 2004, 17: 107-117. 10.1097/00001503-200404000-00004
Schumacker PT, Long GR, Wood LD: Tissue oxygen extraction during hypovolemia: role of hemoglobin P 50 . J Appl Physiol 1987, 62: 1801-1807.
Chawla LS, Zia H, Gutierrez G, Katz NM, Seneff MG, Shah M: Lack of equivalence between central and mixed venous oxygen saturation. Chest 2004, 126: 1891-1896. 10.1378/chest.126.6.1891
Berridge JC: Influence of cardiac output on the correlation between mixed venous and central venous oxygen saturation. Br J Anaesth 1992, 69: 409-410.
Edwards JD, Mayall RM: Importance of the sampling site for measurement of mixed venous oxygen saturation in shock. Crit Care Med 1998, 26: 1356-1360. 10.1097/00003246-199808000-00020
Reinhart K, Kuhn HJ, Hartog C, Bredle DL: Continuous central venous and pulmonary artery oxygen saturation monitoring in the critically ill. Intensive Care Med 2004, 30: 1572-1578. 10.1007/s00134-004-2337-y
Wolfhard UF, Brinkmann M, Splittgerber FH, Knocks M, Sack S, Piotrowski JA, Schieffer M, Gunnicker M: Myocardial lactate extraction during repeated fibrillation/defibrillation episodes in defibrillator implantation testing. Pacing Clin Electrophysiol 1998, 21: 1795-1801.
Waldau T, Larsen VH, Bonde J, Fogh-Andersen N: Lactate, pH, and blood gas analysis in critically ill patients. Acta Anaesthesiol Scand Suppl 1995, 107: 267-271.
Zar JH: Simple Linear Correlation. In Biostatistical Analysis. 4th edition. Prentice-Hall, Inc; Upper Saddle River, NJ; 1999:377-383.
Bland JM, Altman D: Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986, 1: 307-310.
Weil MH, Michaels S, Rackow EC: Comparison of blood lactate concentrations in central venous, pulmonary artery, and arterial blood. Crit Care Med 1987, 15: 489-490.
Abel ED: Glucose transport in the heart. Front Biosci 2004, 9: 201-215.
Dhainaut JF, Huyghebaert MF, Monsallier JF, Lefevre G, Dall'Ava-Santucci J, Brunet F, Villemant D, Carli A, Raichvarg D: Coronary hemodynamics and myocardial metabolism of lactate, free fatty acids, glucose, and ketones in patients with septic shock. Circulation 1987, 75: 533-541.
Stanley WC, Chandler MP: Energy metabolism in the normal and failing heart: potential for therapeutic interventions. Heart Fail Rev 2002, 7: 115-130. 10.1023/A:1015320423577
Crittenden MD: Intraoperative metabolic monitoring of the heart: I. Clinical assessment of coronary sinus metabolites. Ann Thorac Surg 2001, 72: S2220-S2226. 10.1016/S0003-4975(01)03296-9
Stanley WC, Lopaschuk GD, Hall JL, McCormack JG: Regulation of myocardial carbohydrate metabolism under normal and ischaemic conditions. Potential for pharmacological interventions. Cardiovasc Res 1997, 33: 243-257. 10.1016/S0008-6363(96)00245-3
Peuhkurinen K, Ikaheimo M, Airaksinen J, Huikuri H, Linnaluoto M, Takkunen J: Changes in myocardial energy metabolism in elective coronary angioplasty. Cardiovasc Res 1991, 25: 158-163.
Foltz WD, Merchant N, Downar E, Stainsby JA, Wright GA: Coronary venous oximetry using MRI. Magn Reson Med 1999, 42: 837-848. 10.1002/(SICI)1522-2594(199911)42:5<837::AID-MRM3>3.0.CO;2-8
The George Washington University Medical Center Department of Anesthesiology Research Fund financed the study in its entirety. Preliminary results of the study were presented in abstract form at the 2003 American Thoracic Society International Conference, Seattle, WA, USA.
The authors declare that they have no competing interests.
GG conceived the study, participated in its design, performed statistical analysis and drafted the manuscript. LSC and HZ participated in the design of the study, collected data and helped to draft the manuscript. MGS and NMK conducted the study, collected data and helped to draft the manuscript. All authors read and approved the final manuscript.
About this article
Cite this article
Gutierrez, G., Chawla, L.S., Seneff, M.G. et al. Lactate concentration gradient from right atrium to pulmonary artery. Crit Care 9, R425 (2005). https://doi.org/10.1186/cc3741
- Pulmonary Artery
- Inferior Vena Cava
- Right Atrium
- Myocardial Lactate
- Leave Ventricular Stroke Work Index