- Poster presentation
In vivo leukocyte–endothelium interactions in rat mesenteric microvessels after ischemia/reperfusion and sepsis
Critical Care volume 9, Article number: P41 (2005)
A leukocyte–endothelium interaction is known to be a remarkable event at the beginning of systemic inflammatory response syndrome. The aim of this study was to evaluate leukocyte–endothelium interactions in superfused mesenteric postcapillary venules after hemorrhagic shock/reperfusion and cecal ligation and puncture in rats.
Thirty-six Wistar rats (200–250 g) were submitted to the following interventions: 0 hours, anesthesia with sodium pentobarbital (50 mg/kg i.p.), hemorrhagic shock (MAP ~40 mmHg lasting 1 hour) and reperfusion with lactated Ringer's solution (3 × shed blood) + 25% of the shed blood; 24 hours, anesthesia and cecal ligation and puncture; 48 hours, anesthesia, cecal resection and peritoneal lavage; and 72 hours, anesthesia and intravital microscopy of the mesentery (venule diameter, 15–25 μm).
Data of leukocyte–endothelium interactions in rat mesenteric microcirculation are presented as the mean ± standard deviation (Table 1).
The double-hit model (ischemia/reperfusion and sepsis) induced a severe inflammatory injury similar to sepsis alone. The inflammatory process was overcome by cecal resection and peritoneal lavage. Up to 72 hours of reperfusion with lactated Ringer's solution and 25% of the shed blood volume, inflammation is still evidenced by the increased number of migrated cells in the perivascular tissue.
Supported by PRONEX, FAPESP and UNICID.
Rights and permissions
About this article
Cite this article
Nakagawa, N., Nogueira, R., Sinosaki, S. et al. In vivo leukocyte–endothelium interactions in rat mesenteric microvessels after ischemia/reperfusion and sepsis. Crit Care 9 (Suppl 2), P41 (2005). https://doi.org/10.1186/cc3585
- Systemic Inflammatory Response Syndrome
- Sodium Pentobarbital
- Hemorrhagic Shock
- Remarkable Event