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In vivo leukocyte–endothelium interactions in rat mesenteric microvessels after ischemia/reperfusion and sepsis
Critical Care volume 9, Article number: P41 (2005)
A leukocyte–endothelium interaction is known to be a remarkable event at the beginning of systemic inflammatory response syndrome. The aim of this study was to evaluate leukocyte–endothelium interactions in superfused mesenteric postcapillary venules after hemorrhagic shock/reperfusion and cecal ligation and puncture in rats.
Thirty-six Wistar rats (200–250 g) were submitted to the following interventions: 0 hours, anesthesia with sodium pentobarbital (50 mg/kg i.p.), hemorrhagic shock (MAP ~40 mmHg lasting 1 hour) and reperfusion with lactated Ringer's solution (3 × shed blood) + 25% of the shed blood; 24 hours, anesthesia and cecal ligation and puncture; 48 hours, anesthesia, cecal resection and peritoneal lavage; and 72 hours, anesthesia and intravital microscopy of the mesentery (venule diameter, 15–25 μm).
Data of leukocyte–endothelium interactions in rat mesenteric microcirculation are presented as the mean ± standard deviation (Table 1).
The double-hit model (ischemia/reperfusion and sepsis) induced a severe inflammatory injury similar to sepsis alone. The inflammatory process was overcome by cecal resection and peritoneal lavage. Up to 72 hours of reperfusion with lactated Ringer's solution and 25% of the shed blood volume, inflammation is still evidenced by the increased number of migrated cells in the perivascular tissue.
Supported by PRONEX, FAPESP and UNICID.
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Nakagawa, N., Nogueira, R., Sinosaki, S. et al. In vivo leukocyte–endothelium interactions in rat mesenteric microvessels after ischemia/reperfusion and sepsis. Crit Care 9 (Suppl 2), P41 (2005). https://doi.org/10.1186/cc3585
- Systemic Inflammatory Response Syndrome
- Sodium Pentobarbital
- Hemorrhagic Shock
- Remarkable Event