Skip to content

Advertisement

  • Meeting abstract
  • Open Access

Perfusion pressure changes after ANP administration in preeclampsia: in vitrostudy

  • 1,
  • 2,
  • 1 and
  • 3
Critical Care19982 (Suppl 1) :P065

https://doi.org/10.1186/cc195

  • Published:

Keywords

  • Preeclampsia
  • Perfusion Pressure
  • Atrial Natriuretic Peptide
  • Placental Tissue
  • Sympathetic Innervation

Introduction

Regulation of the blood flow in human placental tissue by changes of the vascular resistance is very particular, because of lack of sympathetic innervation in placental vessels [1]. The number of guanylate-coupled receptors in placental (issues, corresponding to ANP-receptors, has been found to be greater in preeclampsia, than in normal pregnancy [2]. The aim of the study was to investigate the influence of various doses of atrial natriuretic peptide (ANP) on perfusion pressure in preeclampsia.

Methods

Heparinized placentas (n = 14) obtained after preeclamp tic (n = 7; 1st group) and normal (n = 7; 2nd group, control) pregnancies delivered at term by cesarean sections were perfused in vitro using modified Cedard manner. Perfusion pressure, measured continuously, was the main parameter of the vascular status changes. The placental vessels were submaximally preconstricted by continuous infusion of the nitric-oxide synthase inhibitor N-?)-nitro-L-arginine (NOLA; 100 µmol/l, 10 min). Over 120 min depending on the period of experiment (30, 45, 60, 75 min) human synthetic aANP were administered (25, 50, 100, 200 nmol/l, 2 min increments).

Results

Mean value of perfusion pressure increase after NOLA infusion was 5.63 kPa (± SD 0,36), the differences between groups I and II did not reach statistical significance.

Conclusion

The responsiveness of the fetoplacental vasculature to the ANP given in higher doses (100, 200 nmol/l, 2 min increments) is increased in preeclampsia (P < 0.05).
Table

Perfusion pressure decrease (kPa) after a ANP administration (mean values ± SD)

Group

30-32 min

45-47 min

60-62 min

75-77 min

I Preeclampsia

1.01 ± 0.17

1.49 ± 0.22

1.99 ± 0.23

2.33 ± 0.39

II Normal pregnancy

0.94 ± 0.2

1.22 ± 0.19

1.43 ± 0.26

1.79 ± 0.24

Authors’ Affiliations

(1)
Dept. of General & Experimental Pathology, 2nd Faculty of Medicine, University School of Medicine, Warsaw, Poland
(2)
Dept. of Anaesthesia & Intensive Therapy, 2nd Faculty of Medicine, University School of Medicine, Warsaw, Poland
(3)
Dept. of Obstetrics & Gynaecology, 2nd Faculty of Medicine, University School of Medicine, Warsaw, Poland

References

  1. . Am J Obstet Gynecol. 1993, 168: 1616-Google Scholar
  2. . Am J Obstet Gynecol. 1994, 170: 142-147.Google Scholar

Copyright

© Current Science Ltd 1998

Advertisement