- Meeting abstract
Peripheral blood lymphocytes of critically ill patients show signs of late stage apoptosis
Critical Care volume 7, Article number: P034 (2003)
Among the immunological events, apoptosis plays an important role in sepsis. Inhibition of apoptosis in mouse models of sepsis improved survival . Phosphatidyl serin externalisation has been detected in peripheral blood leukocytes of patients suffering from sepsis as an early stage of apoptosis [2, 3]. It has not been shown if they undergo complete apoptosis. Therefore, the aim of the current study was to examine if circulating lymphocytes of septic patients display DNA degradation as a sign of late apoptosis.
Isolated mononuclear cells of 11 critically ill patients (three with severe sepsis) were compared with eight healthy controls. Phosphatydil serin externalisation was evaluated by annexin V binding. Necrotic cells were excluded by propidium iodine stain. DNA fragmentation was detected by TUNEL staining using flow cytometry.
In critically ill patients annexin binding was increased to 12.1 ± 7.5% compared with a basal population of 3.8 ± 0.9% in healthy controls (P < 0.05). The TUNEL-positive population increased from 0.5 ± 0.1% to 2.3 ± 1.5% in critically ill patients (P < 0.05).
The study demonstrates that mononuclear cells of critically ill patients show signs of early apoptosis and to a lesser degree also signs of DNA fragmentation. This may be explained by rapid clearing of phosphatydil serin expressing cells from the circulation by phagocytosis.
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This study was supported by a grant from BONFOR.
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Weber, S., Schewe, JC., Schroeder, S. et al. Peripheral blood lymphocytes of critically ill patients show signs of late stage apoptosis. Crit Care 7, P034 (2003). https://doi.org/10.1186/cc1923
- Severe Sepsis
- Annexin Versus
- Peripheral Blood Lymphocyte
- Septic Patient
- Peripheral Blood Leukocyte