Volume 2 Supplement 1

18th International Symposium on Intensive Care and Emergency Medicine

Open Access

Granulocyte colony-stimulating factor (G-CSF) enhances superoxide production in acute liver failure (ALF): an in vivo effect

  • M Clapperton1,
  • J Wendon1 and
  • N Rolando1
Critical Care19982(Suppl 1):P015

https://doi.org/10.1186/cc145

Published: 1 March 1998

Methods

The in vitro ability of G-CSF to improve neutrophil superoxide production has been previously demonstrated in ALF patients [1]. However, the in vivo effect on neutrophil function is unknown. Therefore G-CSF was given to three groups of ALF patients (n = 6) as a daily infusion at four different dosages (25, 50, 100 and 150 µg/m2). Superoxide production was measured after fMLP stimulation, before and at 24 and 96 h.

Results

See table

G-CSF significantly enhanced superoxide production at 96 h (P < 0.05). Furthermore, this effect was observed at doses below the standard therapeutic dose of 150 µg/m2. Further studies are needed to determine the therapeutic value of G-CSF in the prevention and treatment of infection in ALF patients.

Table

G-CSF

 

Hours after G-CSF infusion

 

µg/m2

0 h

24 h

96 h

25

221.4 (58.1)

267.9(62.7)

382.7 (40.6)

50

308.1 (17.2)

339.3 (56.3)

592.0 (98.7)

100

231.5 (65.2)

225.9 (52.6)

317.5(82.2)

150

217.4 (30.7)

357.0 (62.2)

358.4 (45.4)

Authors’ Affiliations

(1)
Institute of Liver Studies, King's College School of Medicine and Dentistry

References

  1. Rolando N, Clapperton M, et al: Effect of granulocyte colony stimulating factor (G-CSF) on neutrophil function in patients with acute liver failure. J Hepatol. 1996, 25: 100-Google Scholar

Copyright

© Current Science Ltd 1998

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