Volume 19 Supplement 1
Concordance between a new molecular real-time approach and traditional culture in suspected VAP patients
© Clavel et al.; licensee BioMed Central Ltd. 2015
Published: 16 March 2015
Early microbiological documentation may reduce attributable mortality and excessive use of broad-spectrum antibiotics in ventilator-associated pneumonia (VAP). Using bronchoalveolar lavage (BAL) and endotracheal aspirates (ETA), we studied a new molecular biology-based approach to detect and quantify bacteria in less than 3 hours. This prospective multicenter trial aimed at comparing the microbiological results obtained using this molecular protocol (easyMAG® system) and semiquantitative culture in suspected VAP.
ETA and BAL samples were consecutively collected during 10 months in adult patients in four ICUs of France. The molecular method includes a preprocessing liquefaction for ETA before DNA extraction. DNAs were extracted using the easyMAG® system. Real-time PCR (qPCR) was run using the ABI7500FastDx PCR instrument. The results presented here concern: Staphylococcus aureus, Pseudomonas aeruginosa and Enterobacteriaceae. Quantification was performed using qPCR standard curves, by converting the cycle threshold to CFU/ ml.
Concordance between qPCR and culture on BAL/ETA in VAP patients.
Sensitivity and specificity of the new molecular approach for these main bacteria found in VAP could enable targeted first-line antibiotic therapy. In the future, the development of this approach will aim at obtaining a bedside diagnostic in only a few hours.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.