Volume 19 Supplement 1

35th International Symposium on Intensive Care and Emergency Medicine

Open Access

Pyruvate dehydrogenase levels are low in sepsis

  • E Nuzzo1,
  • X Liu1,
  • K Berg1,
  • L Andersen1 and
  • M Doninno1
Critical Care201519(Suppl 1):P33

https://doi.org/10.1186/cc14113

Published: 16 March 2015

Introduction

Pyruvate dehydrogenase (PDH) is a key component of aerobic metabolism. Multiple rodent studies have shown that PDH levels are low in sepsis. This leads to a shift to anaerobic metabolism, resulting in increased lactic acid. Alteration in PDH levels during sepsis, however, has never been studied in humans. The aim of this study was to identify whether PDH levels (activity and quantity) were altered in humans in sepsis.

Methods

We conducted a case-control study at a single urban tertiary care center. We compared PDH levels between sepsis and healthy control subjects by measuring PDH levels in peripheral blood mononuclear cells via a novel assay. We measured PDH levels in control subjects at baseline and in sepsis subjects at 0, 24, 48 and 72 hours.

Results

There were 39 sepsis (age 67 ± 14 years, M ± SD) and 19 control (age 50 ± 12 years) subjects of similar gender (56% and 63% female, respectively) and race (79% and 68% Caucasian, respectively). PDH levels in the sepsis group were significantly lower than the control group at all time points (Figures 1 and 2). After controlling for age, gender, race, and assay plate via multivariable linear regression, the effect of treatment group remained significant. We were unable to control for comorbid illness, which was exclusively concentrated in the sepsis group.

Figure 1

Figure 2

Conclusion

PDH levels are significantly lowered in humans during sepsis when compared with healthy controls, even when controlling for age, race and gender. Further research is needed to determine whether this finding persists after adjustment for comorbid disease, and whether lower PDH levels are associated with clinical outcomes.

Authors’ Affiliations

(1)
Beth Israel Deaconess Medical Center

Copyright

© Nuzzo et al.; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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