Volume 19 Supplement 1

35th International Symposium on Intensive Care and Emergency Medicine

Open Access

Elevated basal levels of circulating activated platelets predict ICU-acquired sepsis and mortality: a prospective study

  • N Layios1
Critical Care201519(Suppl 1):P29

https://doi.org/10.1186/cc14109

Published: 16 March 2015

The Erratum to this article has been published in Critical Care 2015 19:301

Introduction

Platelets are now considered to be immune and inflammatory agents as well as key cells in coagulation, and as such have been implicated in the pathophysiology of sepsis [1]. Thrombocytopenia is associated with sepsis severity and poor prognosis, and hyperactivated platelets probably contribute to microvascular thrombosis and organ failure. In the present study, we evaluated platelet activation markers as potential predictive markers of sepsis and of mortality among four commonly encountered populations of patients admitted to ICUs.

Methods

Ninety-nine non-infected ICU patients were prospectively screened at day 1 (T1) and day 3 (T2) of admission after elective cardiac surgery, trauma, acute neurologic dysfunction or prolonged ventilation (>48 hours). A third sample was drawn when infection was diagnosed (Tx). We evaluated platelet activation by measuring the expression of P-selectin (CD62P) and fibrinogen binding on the cell surface before and after stimulation with major platelet agonists (ADP, collagen, and TRAP) through flow cytometry. Clinical scores were obtained at admission.

Results

Patients who developed sepsis (n = 18) presented with significantly higher platelet fibrinogen binding at T1 compared with patients who did not get infected (basal: P = 0.0014, upon stimulation: P < 0.0035). At T1, ROC AUC for association of basal fibrinogen binding with the occurrence of sepsis was 0.79 (95% CI: 0.68 to 0.89). Elevated basal CD62P expression level was associated with increased 90-day mortality (P = 0.042, ROC AUC = 0.78 (0.64 to 0.88)). Kaplan-Meier survival curves illustrated that mortality was significantly higher after stratification based on T1 basal CD62P level (cutoff MFI>31.56, HR = 13.6, P = 8.23 × 10-6). Multivariate logistic regression analysis using clinical scores (SOFA, APACHE II, SAPS II, SAPS III) indicated that addition of CD62P level or of bound fibrinogen level significantly improved prediction of mortality (odds ratio 1.078, P = 0.003) and sepsis (odds ratio 1.033, P = 0.0012), respectively.

Conclusion

Predisposition to severe infection in selected critically ill medico-surgical adults can be identified on day 1 of admission based on circulating basally activated platelets. Levels of activated platelets may add incremental prognostic information to clinical scoring.

Notes

Authors’ Affiliations

(1)
CHU Sart Tilman

References

  1. de Stoppelaar SF, van't Veer C, van der Poll T: The role of platelets in sepsis. Thromb Haemost. 2014, 11: 666-77.View ArticleGoogle Scholar

Copyright

© Layios; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Advertisement