Role of nonpneumoniae mycoplasma in the pathogenesis of ventilator-associated pneumonia: an in vitro assessment
© Nolan et al.; licensee BioMed Central Ltd. 2014
Published: 3 December 2014
Whole human blood was obtained from healthy donor volunteers and cell types were isolated using diffusion gradients and magnetic labeling as appropriate. Monocytes and macrophages were incubated with M. salivarium for 24 hours before a subsequent LPS stimulus. Macrophage phagocytosis assays were conducted after exposure times of 60 minutes and 24 hours to M. salivarium. Cytokines were measured using ELISA and human cytokine bead array kits.
Although further research is needed, it is interesting that the presence of M. salivarium caused an anti-inflammatory effect as well as impaired antigen presentation secondary to impaired phagocytosis. This could be consistent with the better outcome in mechanically ventilated patients that did not have M. salivarium bacteria detected in their bronchoalveolar lavage washings. Extracellular traps contribute to microbial containment by forming a physical barrier composed of chromatin and cytoplasmic proteins to enhance antimicrobial synergy while minimizing damage to host tissues . It is interesting that M. salivarium induced extracellular traps.
Thanks to supervisors ACM and Prof AR for their support and expertise. Thanks also to all the staff in the Centre for Inflammation Research for their help throughout the year and for being so willing to help when help was needed.
- Razin S, Yogev D, Naot Y: Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev 1998, 62: 1094-1156.PubMedPubMed CentralGoogle Scholar
- Wilkinson TS, et al.: Ventilator-associated pneumonia is characterized by excessive release of neutrophil proteases in the lung. Chest 2012, 142: 1425-1432. 10.1378/chest.11-3273View ArticlePubMedGoogle Scholar
- Papayannopoulos V, Zychlinsky A: NETs: a new strategy for using old weapons. Trends Immunol 2009, 30: 513-521. 10.1016/j.it.2009.07.011View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.