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Role of nonpneumoniae mycoplasma in the pathogenesis of ventilator-associated pneumonia: an in vitro assessment
© Nolan et al.; licensee BioMed Central Ltd. 2014
Published: 3 December 2014
Whole human blood was obtained from healthy donor volunteers and cell types were isolated using diffusion gradients and magnetic labeling as appropriate. Monocytes and macrophages were incubated with M. salivarium for 24 hours before a subsequent LPS stimulus. Macrophage phagocytosis assays were conducted after exposure times of 60 minutes and 24 hours to M. salivarium. Cytokines were measured using ELISA and human cytokine bead array kits.
Although further research is needed, it is interesting that the presence of M. salivarium caused an anti-inflammatory effect as well as impaired antigen presentation secondary to impaired phagocytosis. This could be consistent with the better outcome in mechanically ventilated patients that did not have M. salivarium bacteria detected in their bronchoalveolar lavage washings. Extracellular traps contribute to microbial containment by forming a physical barrier composed of chromatin and cytoplasmic proteins to enhance antimicrobial synergy while minimizing damage to host tissues . It is interesting that M. salivarium induced extracellular traps.
Thanks to supervisors ACM and Prof AR for their support and expertise. Thanks also to all the staff in the Centre for Inflammation Research for their help throughout the year and for being so willing to help when help was needed.
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