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Anti-inflammatory and antioxidant effects of ranolazine on primary cultured astrocytes


Because of its ability to block late INa [1], ranolazine is used as an antianginal agent for the treatment of chronic angina pectoris when angina is not adequately controlled by other agents [2]. Besides its cardiovascular effects, ranolazine improves different neuronal functions, and thus its use has been proposed for the treatment of pain and epileptic disorders [3, 4]. Since astrocytes are involved in neuronal inflammatory processes, and autoimmune and neurodegenerative diseases [5], we have investigated the antiinflammatory and antioxidant effects of ranolazine in primary cultured astrocytes.


We incubated differentiated rat astrocytes in primary culture (10 days of culture) [5] for 24 hours with ranolazine (10-5, 10-6, 10-7 M). We measured the protein expression levels of PPARy and Cu/Zn-SOD by western blot technique. Protective effect of ranolazine on cell viability was assayed using MTT conversion assay. Finally, to evaluate the effect of ranolazine on the IL-1β cytokine and TNFα mediators, we used the enzyme-linked immunosorbent assay technique.


Compared with control cells, treatment with ranolazine induced an increment of anti-inflammatory PPARy and reduced the proinflammatory mediators IL-1 β and TNFα in primary cultured astrocytes. Ranolazine (10-6 M) also increased the expression of antioxidant protein Cu/Zn-SOD and caused a significant increase in cell viability.


Ranolazine decreases inflammatory mediators IL-1 β and TNFα, and increases anti-inflammatory PPARy as well as the antioxidant Cu/Zn-SOD in astrocytes in culture. These results suggest that ranolazine could be useful as a neuroprotective drug in pathologies inducing inflammatory damage and oxidant processes.


  1. Belardinelli L, et al.: J Pharmacol Exp Ther. 2013, 344: 23-32. 10.1124/jpet.112.198887

    Article  CAS  PubMed  Google Scholar 

  2. Siddiqui MA, et al.: Drugs. 2006, 66: 693-710. 10.2165/00003495-200666050-00010

    Article  CAS  PubMed  Google Scholar 

  3. Park YY, et al.: J Neurophysiol. 2013, 109: 1378-1390. 10.1152/jn.00435.2012

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Ka h, lig KM, et al.: Br J Pharmacol. 2010, 161: 1414-1426. 10.1111/j.1476-5381.2010.00976.x

    Article  Google Scholar 

  5. Vallés SL, et al.: Brain Pathol. 2004, 14: 365-371.

    Article  PubMed  Google Scholar 

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El Amrani, F., Guerra, S., Aguirre-Rueda, D. et al. Anti-inflammatory and antioxidant effects of ranolazine on primary cultured astrocytes. Crit Care 18 (Suppl 1), P447 (2014).

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