- Poster presentation
- Open Access
Study of the ex vivo immune response of polytrauma older patients in the ICU on admission: preliminary results
Critical Care volume 18, Article number: P236 (2014)
Immunological status is differentiated with age, influencing treatment and outcome . The aim is to determine the immune response of severely traumatized older patients compared with a group with arterial disease, expressed by proinflammatory cytokine release after ex vivo whole-blood LPS stimulation .
The study comprised 16 polytrauma patients admitted to the ICU, aged 78 ± 8 (Group I) and 16 with arterial disease, aged 74 ± 5 (Group II). Ten milliliters of peripheral blood were collected from each patient, divided into two tubes with/without anticoagulant. Diluted 1:10 whole-blood samples were stimulated with 500 pg/ml LPS, at 37°C, for 4 hours. Serum and cell culture supernatants (CCSP) were removed and stored at -70°C. TNFα and IL-6 were measured in serum and CCSP by ELISA.
Serum proinflammatory cytokines were significantly elevated after severe trauma against control group (TNFα, P < 0.001 and IL-6, P < 0.001). Ex vivo cytokine release showed the opposite direction. There was a significantly lower TNFα and IL-6 release for Group I (TNFα, P < 0.05 and IL-6, P < 0.01) compared with Group II. TNFα ex vivo release from the samples of Group II was >300 pg/ml. See Figures 1 and 2.
Older patients showed adequate immunological response, considering the limit of 300 pg/ml. The incidence of severe trauma was involved in the downregulation of immune activity and should be considered. Group I patients do not have the opportunity to precondition their immune status. Group II patients can better compensate operative therapies.
Bruno L: Proc Nutr Soc. 1999, 58: 85-98. 10.1079/PNS19990013
Myrianthefs P, et al.: Cytokine. 2003, 24: 286-292. 10.1016/j.cyto.2003.08.005
About this article
Cite this article
Filippou, L., Venetsanou, K., Voulalas, G. et al. Study of the ex vivo immune response of polytrauma older patients in the ICU on admission: preliminary results. Crit Care 18, P236 (2014). https://doi.org/10.1186/cc13426
- Proinflammatory Cytokine
- Culture Supernatant
- Immune Activity
- Arterial Disease
- Immune Status