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Critical Care

Open Access

Study of the ex vivo immune response of polytrauma older patients in the ICU on admission: preliminary results

  • L Filippou1,
  • K Venetsanou1,
  • G Voulalas1,
  • D Markopoulou1,
  • D Chroni1,
  • C Maltezos1 and
  • I Alamanos1
Critical Care201418(Suppl 1):P236

Published: 17 March 2014


Immunological status is differentiated with age, influencing treatment and outcome [1]. The aim is to determine the immune response of severely traumatized older patients compared with a group with arterial disease, expressed by proinflammatory cytokine release after ex vivo whole-blood LPS stimulation [2].


The study comprised 16 polytrauma patients admitted to the ICU, aged 78 ± 8 (Group I) and 16 with arterial disease, aged 74 ± 5 (Group II). Ten milliliters of peripheral blood were collected from each patient, divided into two tubes with/without anticoagulant. Diluted 1:10 whole-blood samples were stimulated with 500 pg/ml LPS, at 37°C, for 4 hours. Serum and cell culture supernatants (CCSP) were removed and stored at -70°C. TNFα and IL-6 were measured in serum and CCSP by ELISA.


Serum proinflammatory cytokines were significantly elevated after severe trauma against control group (TNFα, P < 0.001 and IL-6, P < 0.001). Ex vivo cytokine release showed the opposite direction. There was a significantly lower TNFα and IL-6 release for Group I (TNFα, P < 0.05 and IL-6, P < 0.01) compared with Group II. TNFα ex vivo release from the samples of Group II was >300 pg/ml. See Figures 1 and 2.
Figure 1

Serum proinflammatory cytokines in older patients.

Figure 2

Ex vivo proinflammatory cytokine release after whole-blood LPS stimulation in older patients.


Older patients showed adequate immunological response, considering the limit of 300 pg/ml. The incidence of severe trauma was involved in the downregulation of immune activity and should be considered. Group I patients do not have the opportunity to precondition their immune status. Group II patients can better compensate operative therapies.

Authors’ Affiliations

KAT Hospital Athens, Athens, Greece


  1. Bruno L: Proc Nutr Soc. 1999, 58: 85-98. 10.1079/PNS19990013View ArticleGoogle Scholar
  2. Myrianthefs P, et al.: Cytokine. 2003, 24: 286-292. 10.1016/j.cyto.2003.08.005View ArticlePubMedGoogle Scholar


© Filippou et al.; licensee BioMed Central Ltd. 2014

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