Study of the ex vivo immune response of polytrauma older patients in the ICU on admission: preliminary results
© Filippou et al.; licensee BioMed Central Ltd. 2014
Published: 17 March 2014
Immunological status is differentiated with age, influencing treatment and outcome . The aim is to determine the immune response of severely traumatized older patients compared with a group with arterial disease, expressed by proinflammatory cytokine release after ex vivo whole-blood LPS stimulation .
The study comprised 16 polytrauma patients admitted to the ICU, aged 78 ± 8 (Group I) and 16 with arterial disease, aged 74 ± 5 (Group II). Ten milliliters of peripheral blood were collected from each patient, divided into two tubes with/without anticoagulant. Diluted 1:10 whole-blood samples were stimulated with 500 pg/ml LPS, at 37°C, for 4 hours. Serum and cell culture supernatants (CCSP) were removed and stored at -70°C. TNFα and IL-6 were measured in serum and CCSP by ELISA.
Older patients showed adequate immunological response, considering the limit of 300 pg/ml. The incidence of severe trauma was involved in the downregulation of immune activity and should be considered. Group I patients do not have the opportunity to precondition their immune status. Group II patients can better compensate operative therapies.
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