Volume 18 Supplement 1
Differential effect of alcohol on TNFα receptor II production in the presence of LPS challenge ex vivo
© Gavala et al.; licensee BioMed Central Ltd. 2014
Published: 17 March 2014
Acute alcohol exposure suppresses proinflammatory response, which may be related to increased susceptibility to infections . The purpose of the study was to investigate the effect of acute exposure to alcohol on TNFα production capacity and TNFα receptors (TNFRs) in an ex vivo model of whole-blood stimulation with lipopolysaccharide (LPS).
Whole blood was taken from healthy volunteers and was placed in tubes containing EDTA and immediately transferred to the laboratory. Heparinized blood samples were diluted 1:10 in RPMI 1640 culture medium (100 μl whole blood added in 900 μl RPMI 1640). Samples were preincubated with 0, 5, 12.5, 25, 50, 100 and 200 mM alcohol (EtOH) for 10 minutes at room temperature. After incubation, 500 pg LPS was added to each sample for 4 hours at 37°C. At the end of the process, samples were centrifuged (1,800 rpm, 5 minutes, r.t.). Culture supernatants were collected and stored at -70°C until measurements. TNFα and TNFR levels were determined in culture supernatant using the ELISA method .
Our observations indicate a suppression of proinflam- matory response, but also a differential effect of alcohol on TNFR II production of whole blood in the presence of LPS challenge depending on the degree of alcohol intoxication.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.