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BXL 628 ameliorates toxicity of lactated Ringer in HK-2 human renal proximal tubule cells in a hypovolemia mimicking model
Critical Care volume 18, Article number: P156 (2014)
Lactated Ringer (L/R) for resuscitation of hemorrhagic shock is suggested by the ATLS program. However, prior studies showed that resuscitation with L/R was associated with more kidney damage in rats with severe hemorrhagic shock. The direct effects of L/R on human renal tubule cells have not been reported.
Human proximal renal tubular cell line HK-2 was used. Viability was examined by MTT assay. An additional equal volume of phosphate- buffered saline (PBS) served as control. Addition of 200 nM dipyridyl and 1 mM H2O2 served as conditions of hypoxia and oxidative stress, respectively. Patterns of cell death were observed by flow cytometry.
To imitate early resuscitation in hypovolemic shock, medium was replaced with 40% v/v of L/R with dipyridyl plus H2O2 for 4 hours, followed by replacing with complete medium for 44 hours. In such conditions, L/R augmented cytotoxicity, and more annexin V (+) cells were observed. Co-treatment or post-treatment with BXL 628, a novel VDR agonist, reversed L/R-induced cytotoxicity. See Figures 1 and 2.
BXL 628 rescued L/R-induced apoptosis in human renal proximal tubule cells in a hypovolemia mimicking model.
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Huang, Y., Cheng, C., Lin, T. et al. BXL 628 ameliorates toxicity of lactated Ringer in HK-2 human renal proximal tubule cells in a hypovolemia mimicking model. Crit Care 18, P156 (2014). https://doi.org/10.1186/cc13346
- Annexin Versus
- Hemorrhagic Shock
- Renal Tubule
- Tubule Cell
- Lactate Ringer