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Study of the effect of C1-esterase inhibitor administration to the sepsis pig model
Critical Care volume 17, Article number: P86 (2013)
New therapy is required that improves the prognosis of patients suffering from severe sepsis or septic shock. C1-esterase inhibitor (C1-Inh) was introduced in clinical medicine for patients with hereditary angioedema. Some studies show that C1-Inh administration may also have a beneficial effect in other clinical conditions such as sepsis [1, 2]. We examined the effect of C1-Inh administration to the sepsis pig model.
Materials and methods
The experiments were performed divided into two groups: the treatment group and the control group. We administered LPS (40 μg/kg) to pigs of about 10 kg over 30 minutes. At the same time, we administered C1-Inh in the control group (500 U, n = 3; 1,000 U, n = 3), and saline in the control group (n = 3). We examined the effect of C1-Inh for the outcome of the two groups, physiological indicators such as heart rates (HR) and mean arterial pressure (MAP), and autopsy results such as pleural effusion and ascites.
The outcome of the two groups was that 5/6 in the treatment group and 2/3 in the control group survived at 240 minutes from the end of LPS administration. HR (/minute) at 180 minutes from the end of LPS administration was 157.5 ± 12.3 in the treatment group and 205.3 ± 42.6 in the control group, and MAP (mmHg) at the same time was 60.0 ± 8.2 in the treatment group and 58.3 ± 5.6 in the control group. As for the autopsy results, pleural effusion (ml) was 13.28 ± 3.13 in the treatment group and 9.87 ± 4.33 in the control group, and ascites (ml) was 165.8 ± 32.99 in the treatment group and 210.0 ± 60.8 in the control group. Seeing each individual, the individual showing a large effect of C1-inh was observed.
C1-Inh tended to stabilize the hemodynamics of the sepsis pig model, but was not able to reduce significantly the amount of pleural effusion and ascites.
Caliezi C, Wuillemin WA, Zeerleder S, Redondo M, Eisele B, Hack CE: C1-esterase inhibitor: an anti-inflammatory agent and its potential use in the treatment of diseases other than hereditary angioedema. Pharmacol Rev 2000, 52: 91-112.
Igonin AA, Protsenko DN, Galstyan GM, Vlasenko AV, Khachatryan NN, Nekhaev IV, Shlyapnikov SA, Lazareva NB, Herscu P: C1-esterase inhibitor infusion increases survival rates for patients with sepsis. Crit Care Med 2012, 40: 770-777. 10.1097/CCM.0b013e318236edb8
This is a collaborative study of Emergency and Critical Care Center, Saga University Hospital and the Department of Veterinary Medicine, Rakuno Gakuen University.
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Imahase, H., Sakamoto, Y., Miyasho, T. et al. Study of the effect of C1-esterase inhibitor administration to the sepsis pig model. Crit Care 17, P86 (2013). https://doi.org/10.1186/cc12985
- Public Health
- Heart Rate
- Treatment Group
- Beneficial Effect
- Septic Shock