The study population was 1,961 patients in whom 3,213 CVCs were utilised over 19,511 CVC days. There were 256 CRBSI episodes in 216 patients. Median (IQR) patient age was 62 (23), and 58% were male. The incidence of CRBSI decreased significantly (P < 0.001) during the study period from 16% of patients in the period 1997 to 2003 to 7% in 2004 to 2010. The corresponding rate of CRBSI infection (per 1,000 CVC days) decreased from 18 to 10. There was a significant decrease (P < 0.001) in numbers of CVCs inserted per patient (from 1.87 to 1.49). Each extra CVC PN day was associated with an increased risk of developing CRBSI of 3.4% (OR 1.034, P < 0.05). The number of PN CVCs was associated with developing CRBSI (OR 1.218, P < 0.10). Patient factors significantly associated with CRBSI included perioperative PN use (compared with medical patients) (OR 2.414, P < 0.01), and male sex (OR 1.952, P < 0.01).