Skip to main content

Bacterial translocation primes proinflammatory responses and is connected to early death in an experimental model of lethal injury

Introduction

Some cases of multiple trauma are rapidly deteriorating; the mechanism was investigated.

Methods

Forty-one rabbits were assigned into two groups; sham-operated and subject to crush of the right femur. Survival was recorded; peripheral blood was sampled for LPS measurement by the kinetic QCL-1000 LAL assay; quantitative tissue growth was assessed after death. Some rabbits were sacrificed at 48 hours; blood was sampled from the portal vein for LPS measurement; splenocytes were isolated and incubated for 24 hours in the presence of 10 ng/gl LPS of Escherichia coli O55:B5 and of 5 μg/ml phytohemagglutin (PHA); TNFα was measured in supernatants by a bioassay on L929 fibroblasts.

Results

Fifty percent of rabbits died early; that is, within the first 48 hours. Mean ± SE log10 bacteria in the liver and lung of animals that died early was 2.27 ± 0.62 and 3.16 ± 0.783cfu/g; respective values of rabbits that started dying late (that is, after 72 hours) were below the limit of detection. Mean circulating LPS at 24 hours was 2.09 EU/ ml and 1.99 EU/ml respectively (P = NS). Mean LPS of the portal vein of the sham and of the injury groups were 1.25 and 5.62 EU/ml (P = 0.047). Concentrations of TNFα in splenocyte supernatants are shown in Figure 1.

Figure 1
figure1

Stimulation of TNFα from isolated splenocytes.

Conclusion

Early death after injury is not related to peripheral endotoxemia and sepsis; bacterial translocation priming for enhanced proinflammatory responses is a likely explanation.

Author information

Affiliations

Authors

Corresponding author

Correspondence to N Baxevanos.

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Cite this article

Baxevanos, N., Tsaganos, T., Pistiki, A. et al. Bacterial translocation primes proinflammatory responses and is connected to early death in an experimental model of lethal injury. Crit Care 17, P16 (2013). https://doi.org/10.1186/cc11954

Download citation

Keywords

  • Escherichia Coli
  • Portal Vein
  • Emergency Medicine
  • Early Death
  • Tissue Growth