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Reversible depressive effect of TNFα on a model of isolated perfused rat heart
Critical Care volume 17, Article number: P2 (2013)
After profound anesthesia with pentothal, the Wistar rats were killed by exsanguination. After sternotomy, the heart was taken and connected to the Langendorf column. The apex of the heart was hooked to a strength sensor. Biopac student laboratory software was used to record and analyse heart contractions. Contractions were recorded every 5 minutes during periods of 20 minutes. Control measurements were first recorded. We measured four parameters: heart rate, contraction force, speeds of contraction and relaxation for control, during TNFα (20 ng/ml) exposure and after removal of TNFα. We express the variations of parameters as percentage of the control ± SEM. A paired t test was used to compare heart rate, contraction amplitude, speeds of contraction and relaxation with TNFa and control measurements and after removal of TNFα.
Eight rat hearts Wistar (weight = 325 ± 23 g) were studied. See Table 1.
TNFα decreases significantly the heart rate, contractile force, speeds of contraction and relaxation on isolated perfused rat heart. TNFα probably plays a role in the pathophysiology of cardiomyopathy during septic shock. The partial reversibility of these effects could explain why left ventricular hypokinesia in patients with septic shock is reversible.
Vieillard-Baron A, et al.: Actual incidence of global left ventricular hypokinesia in adult septic shock. Crit Care Med 2008, 36: 1701-1706. 10.1097/CCM.0b013e318174db05
Hunter JD, et al.: Sepsis and the heart. Br J Anaesth 2010, 104: 3-11. 10.1093/bja/aep339
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Nguyen, B., Guillouet, M., Giroux-Metges, M. et al. Reversible depressive effect of TNFα on a model of isolated perfused rat heart. Crit Care 17, P2 (2013). https://doi.org/10.1186/cc11940
- Septic Shock
- Control Measurement
- Depressive Effect