Contribution of activated platelets to plasma IL-27 levels
Critical Care volume 17, Article number: 411 (2013)
In a recent issue of Critical Care, Wong and colleagues  demonstrated that the serum concentration of IL-27 in critically ill children was a predictor of infection. Our study aims at determining whether platelet activation contributes to the elevated plasma IL-27 concentration. Here we demonstrate that activation of platelets with thrombin receptor activating peptide (TRAP) significantly increased IL-27 levels in supernatants (Figure 1a). Moreover, B cells incubated in vitro with supernatants from activated platelets upregulated membrane expression of CD86, which was restored to baseline when B cells were pre-incubated with a gp130 blocking antibody (Figure 1b). Our data strongly suggest that platelet activation contributes, along with classical sources , to elevated plasma levels of IL-27. Recent advances place platelets as an important link between innate and adaptive immunity . Indeed, platelets modulate their inflammatory response after sensing the presence of an infectious agent . Therefore, platelet activation could contribute to increased plasma concentrations of IL-27 along with cytokines such as soluble CD40L , and thus may contribute towards immune dysregulation in patients with sepsis.
The authors thank MA Eyraud and CA Arthaud for technical assistance. This work was supported by the EFS (grant APR2010-No10) the ANR (grant ANR-12- JSV1), the ART, and the 'Les Amis de Rémi' Association; France. In accordance with French regulations, platelets were obtained from regular blood donors who signed a form indicating that they do not preclude the use of their sample for medical research.
thrombin receptor activating peptide.
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The authors declare that they have no competing interests.
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Hamzeh-Cognasse, H., Damien, P., Nguyen, K.A. et al. Contribution of activated platelets to plasma IL-27 levels. Crit Care 17, 411 (2013). https://doi.org/10.1186/cc11925
- Platelet Activation
- Elevated Plasma
- Membrane Expression
- Immune Dysregulation
- Increase Plasma Concentration