- Poster presentation
- Open Access
Abdominal organs' microcirculation dysfunction sequence in severe sepsis by SDF microscopy and histology
© Almeida-Filho et al.; licensee BioMed Central Ltd. 2012
- Published: 14 November 2012
- Severe Sepsis
- Solid Organ
- Abdominal Organ
- Sepsis Group
- Focal Event
The microcirculation dysfunction sequence in sepsis has not been well acknowledged to support therapy and prognosis. Herein, the abdominal organs' microcirculation and their perivascular tissue derangements captured by SDF microscopy were correlated with the whole organ histological findings in severe sepsis.
Adult Wistar-EPM rats (n = 54) were distributed into: sepsis group (n = 36), animals submitted to 109 CFU/ml Escherichia coli inoculation through the jugular vein; and sham group (n = 18), animals with physiologic saline 0.9%. At 0, 2, 6 and 24 hours, liver, kidney and ileum microvascular and perivascular images were monitored by SDF in vivo and also by histology.
Organ dysfunction in sepsis is better detectable in solid organs by SDF imaging as compared with gut muscular compartment. These results demonstrated the importance of the solid organ SDF monitoring in experimental sepsis treatment studies.
Supported by FAPESP.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.